Plant Viruses
Online 
Descriptions and Lists from
the VIDE Database
Bromoviruses:
Bromoviridae
Index
Nomenclature
ICTV reference
Definitive species
Host range and
symptoms
Natural host range and symptoms
Symptoms
persist (5), or none (1).
Transmission
Transmitted by a vector (2/2); an insect
(2/2); Coleoptera (2/2). Virus transmitted by mechanical inoculation;
transmitted by grafting (2/2); transmitted by contact between plants (1/1); not
transmitted by seed (5/5); not transmitted by pollen (1/1).
Experimental host range
Many (>9) families susceptible
(1), or several (3-9) families susceptible (5).
Susceptible
host species
Insusceptible host species
Families containing susceptible hosts
Families containing
insusceptible hosts
Physical and
biochemical properties
Properties of particles in sap
TIP:
67-82.87-95 °C. LIV: 1-23.87-44 days. DEP: log10 minus 3-4 (2/4), or 5 (3/4).
Infectivity of sap increased by treatment with di-ethyl ether (1/2), or not
changed by treatment with di-ethyl ether (1/2). Leaf sap contains many
virions (5/5).
Particle morphology
Virions isometric; not enveloped
(5/5); 25-26.16-28 nm in diameter; rounded in profile (5/5); with a conspicuous
capsomere arrangement (2/5), or without a conspicuous capsomere arrangement
(3/5).
Physical properties
One sedimenting component in purified
preparations (5/5), or three sedimenting components in purified preparations
(1/5); sedimentation coefficient 85-87.06-90 S; of the other(s) 78.7
S. Density 1.35-1.356-1.36 g cm-3 in CsCl. Isoelectric point pH
3.6-4.925-6.8. A260/A280 ratio 1.7-3.55-5.4.
Biochemical properties
Virions contain 20-21.92-23.7 %
nucleic acid; 76.3-77.43-79 % protein; 0 % lipid.
Genome consists of RNA; single-stranded; linear (4/4). Total genome size
8.216-8.622-9.5 kb. Genome of three parts (5/5); largest (or only) genome part
3.158-3.372-3.9 kb; the 2nd largest 2.799-3.032-3.4 kb; the 3rd largest
2.117-2.296-2.6 kb. Base composition 24.4-25.95-28 % G; 24.9-26.05-27 % A;
19-20.62-22.2 % C; 24-27.42-29 % U. 5´ terminus of RNA has a methylated
nucleotide cap (3/3). Infectivity retained when deproteinised with proteases
(1/1), or decreased when deproteinised with proteases (1/1); retained when
deproteinised with phenol or detergent (3/3). Poly A region absent (1/1).
Additional factor not required for infectivity (2/2). Genome has
tRNA-like activity (3/3).
Features of the genome
Non-genomic nucleic acid
found in the virions (5/5); is subgenomic mRNA (4/4). Sub-genomic
mRNA found in infected cells (3/3). 5 virus specified dsRNA species found
in infected cells. Size of largest virus specified dsRNA 2.2 kbp; 2nd largest 2
kbp; 3rd largest 1.4 kbp; 4th largest 1.2 kbp; 5th largest 0.5 kbp.
Features of proteins
Replication
Genome replicates in cytoplasm (1/1).
Replication does not depend on a helper virus (5/5).
Cytopathology
Virions found in cytoplasm (5/5), or in
nuclei (1/5), or in the perinuclear space (1/5), or in chloroplasts (1/5), or in
cell vacuoles (1/5). Inclusions present in infected cells (3/4), or absent from
infected cells (1/4); are crystals in the cytoplasm (1/3), or amorphous
X-bodies (1/3), or unusual in shape (1/3); they contain virions (2/2).
Taxonomy and
relationships
Viral family : Bromoviridae.
Illustrations
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
(1996 onwards).
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
http://biology.anu.edu.au/Groups/MES/vide/
Dallwitz (1980)
and
Dallwitz, Paine and Zurcher (1993)
should also be cited.
Please send comments, corrections and suggestions to:
vide-manager@biology.anu.edu.au
