Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Voandzeia necrotic mosaic tymovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt and A. Monsarrat, 1988.

Nomenclature

Acronym

VNMV

ICTV decimal code

77.0.1.0.017

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Vigna subterranea - mosaic, necrosis and malformation of leaves, occasionally lethal.

Transmission

Possibly transmitted by a vector. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by seed.

Ecology and control

Studies reported by Monsarrat et al. (1984).

Geographical distribution

Spreads in Cote d'Ivoire.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Vigna subterranea - systemic chlorosis, veinal necrosis and leaf malformation.
• Lablab purpureus, Vigna unguiculata ssp. unguiculata - chlorotic local lesions, then systemic leaf chlorosis.
• Chenopodium amaranticolor - necrotic local lesions; not systemic.

Diagnostically insusceptible host species

Arachis hypogaea, Phaseolus vulgaris, Nicotiana benthamiana, Nicotiana glutinosa, Abelmoschus esculentus.

Maintenance and propagation hosts

Vigna subterranea, Lablab purpureus, Vigna unguiculata ssp. unguiculata.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L).

Susceptible host species

• Chenopodium amaranticolor
• Lablab purpureus
• Vigna subterranea
• Vigna unguiculata
• Vigna unguiculata ssp. cylindrica
• Vigna unguiculata ssp. sesquipedalis
• Vigna unguiculata ssp. unguiculata

Insusceptible host species

• Abelmoschus esculentus
• Amaranthaceae
• Arachis hypogaea
• Chenopodiaceae
• Cruciferae
• Leguminosae
• Malvaceae
• Nicotiana benthamiana
• Nicotiana glutinosa
• Passifloraceae
• Phaseolus vulgaris
• Solanaceae

Families containing susceptible hosts

• Chenopodiaceae (1/1)
• Leguminosae-Papilionoideae (6/8)

Families containing insusceptible hosts

• Amaranthaceae
• Chenopodiaceae
• Cruciferae
• Leguminosae
• Leguminosae-Papilionoideae (2/8)
• Malvaceae (1/1)
• Passifloraceae
• Solanaceae (2/2)

Comments on host-range

An unspecified 82 species in Amaranthaceae, Chenopodiaceae, Cruciferae, Leguminosae, Malvaceae, Passifloraceae and Solanaceae are not infected.

Sources of host-range data

Monsarrat et al. (1984).

Physical and biochemical properties

Properties of particles in sap

TIP: in Vigna subterranea sap 70 °C. LIV: 2 days at 10ºC and 10 days (at 4ºC or -20ºC). DEP: log₁₀ minus 2. Leaf sap contains few virions.

Purification method

Monsarrat et al. (1984).

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 113 S (B); of the other(s) 51 S (T). Density 1.45 g cm^-3 in CsCl (B), or 1.29 g cm^-3 in CsCl (T). Isoelectric point pH 4.65. A₂₆₀/A₂₈₀ ratio 1.75 (B), or 0.64 (T).

Biochemical properties

Virions contain 35 % nucleic acid (B); 65 % protein (B).

Genome consists of RNA; single-stranded.

Features of proteins

Virion protein(s) one; M_r 20000; coat protein.

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm; they contain virions. Other cellular changes: the occurence of peripheral vesicles in chloroplasts bounded by a double membrane.

Taxonomy and relationships

Tymovirus

Virus(es) with serologically related virions

Kennedya yellow mosaic, desmodium yellow mottle, okra mosaic and turnip yellow mosaic viruses.

Virus(es) with serologically unrelated virions

Cacao yellow mosaic and clitoria yellow vein viruses.

Comments and References

References

• Fauquet, C., Monsarrat, A. and Thouvenel, J.-C. (1981). Abstr. 5th. Congr. Virol., Strasbourg, 1981, p. 273.
• Fauquet, C., Monsarrat, A. and Thouvenel, J.-C. (1984). CMI/AAB Descr. Pl. Viruses No. 279, 4 pp.
• Monsarrat, A., Fauquet, C. and Thouvenel, J.-C. (1984). C.r. hebd. Seanc. Acad. Sci. Paris, Series III299: 53.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.