Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Turnip crinkle carmovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt, 1991.

Nomenclature

Acronym

TCV

ICTV decimal code

74.0.2.0.012

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Brassica campestris ssp. rapa - mottling and leaf distortion; stunting of plants.
• Brassica napus var. napobrassica - faint leaf chlorosis or symptomless infection.
• Brassica sinapis - severe leaf distortion.

Transmission

Transmitted by a vector; an insect; Phyllotreta (nine species) and Psylloides (two species). Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in the UK and the former Yugoslavia.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Brassica campestris ssp. pekinensis - local chlorotic lesions; systemic leaf mottling and crinkling.
• Chenopodium amaranticolor, C. quinoa, Datura stramonium - local chlorotic lesions; no systemic infection.
• Chenopodium murale - local chlorotic lesions; systemic chlorotic flecking.
• Tetragonia tetragonioides - local chlorotic lesions; symptomless systemic infection.

Maintenance and propagation hosts

Brassica juncea, Brassica campestris ssp. pekinensis.

Assay hosts (Local lesions or Whole plants)

Brassica juncea (W), Brassica campestris ssp. pekinensis (W), Chenopodium amaranticolor (L), C. murale (L), C. quinoa (L), Tetragonia tetragonioides (L).

Susceptible host species

• Brassica campestris ssp. napus
• Brassica campestris ssp. pekinensis
• Brassica campestris ssp. rapa
• Brassica juncea
• Brassica napus var. napobrassica
• Brassica oleracea var. botrytis
• Brassica oleracea var. capitata
• Brassica sinapis
• Capsella bursa-pastoris
• Catharanthus roseus
• Cheiranthus cheiri
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Datura stramonium
• Gomphrena globosa
• Lactuca sativa
• Lunaria annua
• Lycopersicon esculentum
• Nicotiana bigelovii
• Raphanus sativus
• Sinapis alba
• Tetragonia tetragonioides

Insusceptible host species

• Amaranthus caudatus
• Beta vulgaris
• Nicotiana clevelandii
• Nicotiana tabacum
• Phaseolus vulgaris
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/2)
• Apocynaceae (1/1)
• Chenopodiaceae (4/5)
• Compositae (1/1)
• Cruciferae (13/13)
• Cucurbitaceae (1/1)
• Solanaceae (3/5)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Amaranthaceae (1/2)
• Chenopodiaceae (1/5)
• Leguminosae-Papilionoideae (2/2)
• Solanaceae (2/5)

Sources of host-range data

Broadbent and Heathcote (1958).

Physical and biochemical properties

Properties of particles in sap

TIP: 95 °C. LIV: more than 42 days (at 20ºC). DEP: log₁₀ minus 6.

Purification method

Hollings and Stone (1969).

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 129 S. Density 1.34 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.48.

Biochemical properties

Virions contain 17 % nucleic acid; 83 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 4.2 kb. Genome unipartite; largest (or only) genome part 4.2 kb. Poly A region absent. Nucleotide sequence references: Carrington et al. (1987).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• M22445 Em(40)_vi:TCVPORF Gb(84)_vi:TCVPORF Turnip crinkle virus P28, P88, P8 and coat protein genes RNA, complete cds. 11/89 4,050bp
• M29290 Em(40)_vi:TCVDIGAA Gb(84)_vi:TCVDIGAA Turnip crinkle virus defective interfering RNA. 5/90 347bp.
• X05193 Em(40)_vi:TOTCVCP Gb(84)_vi:TOTCVCP Turnip crinkle virus RNA for coat protein. 9/93 1,450bp.
• X12749 Em(40)_vi:TOTCVRF Gb(84)_vi:TOTCVRF Turnip crinkle virus (TCV) avirulent satellite RNA F. 12/89 230bp.
• X12750 Em(40)_vi:TOTCVRC Gb(84)_vi:TOTCVRC Turnip crinkle virus (TCV) virulent satellite RNA C. 4/89 355bp. 5 sequences.

Features of proteins

Virion protein(s) one; M_r 38000; coat protein. Amino acid sequence: Carrington (1986); Carrington et al. (1987). Amino acid composition: Butler (1970).

Cytopathology

Virions found in all parts of the host plant. Inclusions absent from infected cells.

Taxonomy and relationships

Carmovirus: Tombusviridae

Virus(es) with serologically related virions

Galinsoga mosaic virus. Suggested relationship to carnation mottle carmovirus not yet confirmed.

Virus(es) with serologically unrelated virions

Carnation mottle virus.

Additional comments on relationships

The virus showed no RNA sequence homology with galinsoga mosaic, cucumber soil-borne, saguaro cactus or pelargonium flower break viruses.

Comments and References

References

• Altenbach, S.B. and Howell, S.H. (1982). Virology 118: 128.
• Broadbent, L. and Blencowe, J.W. (1955). Rep. Rothamsted Exp. Stn. 1954, p. 87.
• Broadbent, L. and Heathcote, G.D. (1958). Ann. appl. Biol. 46: 585.
• Butler, P.J.G. (1970). J. mol. Biol. 52: 589.
• Carrington, J.C. (1986). Ph.D. Thesis. University of California, Berkeley, U.S.A.
• Carrington, J.C., Heaton, L.A., Zuidema, D., Hillman, B.I. and Morris, T.J. (1989). Virology 170: 219.
• Carrington, J.C., Morris, T.J., Stockley, P.G. and Harrison, S.C. (1987). J. mol. Biol. 194: 265.
• Dougherty, W.G. and Kaesberg, P. (1981). Virology 115: 45.
• Finch, J.T., Klug, A. and Leberman, R. (1970). J. mol. Biol. 50: 215.
• Hacker, D.L., Petty, I.R.D., Wei, N. and Morris, T.J. (1992). Virology 186: 1.
• Hollings, M. and Stone, O.M. (1969). Zbl. Bakt. ParasitKde. Abt. 2, 123: 237.
• Leberman, R. and Finch, J.T. (1970). J. mol. Biol. 50: 209.
• McLeod, R. and Markham, R. (1963). Virology 19: 190.
• Martin, C. (1958). Proc. 3rd Conf. Potato Virus Dis., Lisse-Wageningen, 1957, p. 106.
• Symons, R.H., Rees, M.W., Short, M.N. and Markham, R. (1963). J. mol. Biol. 6: 1.
• Tremaine, J. (1970). Virology 42: 611.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.