Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Tulip breaking potyvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, 1986.

Nomenclature

Synonyms

lily mosaic virus, lily mottle virus, lily streak and tulip mosaic virus.

Acronym

TBV

Strains

severe tulip breaking virus; mild tulip breaking virus.

ICTV decimal code

57.0.1.0.070

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Tulipa spp. and Darwin hybrids - colour-breaking of flowers and leaf chlorosis.
• Lilium spp. and Mid-Century hybrids - leaf chlorosis and degeneration.

Transmission

Transmitted by a vector; an insect; Myzus persicae, Aphis gossypii, A. fabae, Macrosiphum euphorbiae, Dysaphis tulipae, Aulocorthum circumflexum; Aphididae. Transmitted in a non-persistent manner. Virus does not require a helper virus for vector transmission; transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Probably distributed worldwide.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Lilium formosanum - mottle, malformed leaves and flowers.
• Tulipa hybrids - flower colour- breaking.

Diagnostically insusceptible host species

Not known to infect any herbaceous indicator species. Narcissus pseudonarcissus, Hyacinthus orientalis and Hippeastrum hybridum.

Maintenance and propagation hosts

Tulipa varieties Croisette, Grand Pride and Paljas. Lilium varieties Concorde and Sterling Star.

Assay hosts (Local lesions or Whole plants)

none known, but Lilium formosanum (W) could be used.

Susceptible host species

• Calochortus
• Fritillaria pudica
• Lilium (cvs Concorde, Sterling Star)
• Lilium formosanum
• Lilium longiflorum
• Ornithogalum thyrsoides
• Tulipa
• Tulipa hybrids
• Zigadenus fremontii

Insusceptible host species

• Allium cepa
• Amaranthus retroflexus
• Atriplex hortensis
• Avena sativa
• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Crotalaria spectabilis
• Cucumis sativus
• Datura stramonium
• Glycine max
• Gomphrena globosa
• Hippeastrum hybridum
• Hyacinthus orientalis
• Narcissus pseudonarcissus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Saccharum officinarum
• Secale cereale
• Tetragonia tetragonioides
• Torenia fournieri
• Vicia faba
• Vigna unguiculata
• Zea mays
• Zinnia elegans

Families containing susceptible hosts

• Calochortaceae (1/1)
• Hyacinthaceae (1/2)
• Liliaceae (6/6)
• Melanthiaceae (1/1)

Families containing insusceptible hosts

• Alliaceae (1/1)
• Amaranthaceae (2/2)
• Amaryllidaceae (2/2)
• Chenopodiaceae (4/4)
• Compositae (1/1)
• Cucurbitaceae (1/1)
• Gramineae (4/4)
• Hyacinthaceae (1/2)
• Leguminosae-Papilionoideae (5/5)
• Scrophulariaceae (1 /1)
• Solanaceae (5/5)
• Tetragoniaceae (1/1)

Comments on host-range

Brierley and Smith (1944) inoculated 79 dicotyledonous species from 66 genera and 22 families with tulip breaking virus, and back tested by inoculation to Lilium formosanum. All tests were negative, but the species were not named, and in a further survey, 39 monocotyledous species also proved to be insusceptible.

Sources of host-range data

Brierley and Smith (1944).

Physical and biochemical properties

Properties of particles in sap

TIP: 65-70 °C. LIV: 4-6 days (in lily sap). DEP: log₁₀ minus 5. Leaf sap contains few virions.

Purification method

Derks et al. (1982).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 750-775 nm; 14 nm wide. Axial canal obscure. Basic helix obscure.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 153 S. A₂₆₀/A₂₈₀ ratio 1.03-1.21.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size c. 10 kb. Nucleotide sequence references: Langeveld et al., 1991; 1586 nucleotides of the 3´terminal end of the genome of a lily isolate.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• S44147 Em(40)_vi:S44147 Gb(84)_vi:S44147 polyprotein precursor tulip breaking virus, lily, Genomic RNA, 1556 nt. 1/94 1,556bp.
• S60804 Em(40)_vi:S60804 Gb(84)_vi:S60804 coat protein tulip- breaking virus TBV, Genomic RNA, 277 nt. 1/94 277bp.
• S60808 Em(40)_vi:S60808 Gb(84)_vi:S60808 coat protein Rembrandt tulip-breaking virus ReTBV, Genomic RNA, 277 nt. 1/94 277bp.
• X63630 Gb(84)_vi:TMVCPRNA Tulip mosaic virus gene for coat protein. 7/94 1,479bp.
• S60806 Em(40)_vi:S60806 Gb(84)_vi:S60806 coat protein tulip top-breaking virus TTBV, Genomic RNA, 277 nt. 1/94 277bp.
• S60810 Em(40)_vi:S60810 Gb(84)_vi:S60810 coat protein lily mottle virus LiMV, Genomic RNA, 277 nt. 1/94 277bp. 6 sequences.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm (scattered, or in bundles, but not in chloroplasts or mitochondria). Inclusions present in infected cells; are crystals in the cytoplasm, or viroplasms (N.B. these differ from the X-bodies produced by lily symptomless virus ; McWhorter, 1940), or pinwheels.

Taxonomy and relationships

Potyvirus: Potyviridae

Virus(es) with serologically related virions

Tobacco etch (SDI: 5) and henbane mosaic viruses (SDI: 7).

Virus(es) with serologically unrelated virions

Alstroemeria mosaic, bean yellow mosaic, clover yellow vein, Columbian datura, hippeastrum mosaic, iris mild mosaic, lettuce mosaic, narcissus yellow stripe, potato A, potato Y or turnip mosaic viruses.

Comments and References

References

• Brierley, P. and Smith, F.F. (1944). Phytopathology 34: 718.
• Cayley, D.M. (1928). Ann. appl. Biol. 15: 529.
• Cayley, D.M. (1932). Ann. appl. Biol. 19: 153.
• Derks, A.F.L.M., Vink-van den Abeele, J.L. and van Schadewijk, A.R. (1982). Neth. J. Pl. Path. 88: 87.
• Langeveld, S.A., Dore, J.-M., Memelink, J., Derks, A.F.L.M., van der Vlugt, C.I.M., Asjes, C.J. and Bol, J.F. (1991). J. gen. Virol. 72: 1531.
• McWhorter, F.P. (1940). Phytopathology 30: 788.
• Van Slogteren, D.H.M. (1971). CMI/AAB Descr. Pl. Viruses No. 71, 4 pp.
• Van Slogteren, D.H.M. and de Bruyn Ouboter, M.P. (1941). Meded. Landb. Wageningen 45: 54.
• Van Slogteren, D.H.M. and de Vos, N.P. (1966). In: Viruses of Plants, pp. 320; eds. A.B.R. Beemster and J. Dijkstra. North Holland Publishing Co., Amsterdam.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.