Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Tomato Australian leafcurl bigeminivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by B.D. Conde and J.E. Thomas, 1987.

Nomenclature

Synonyms

tomato transmissible leafroll virus.

Acronym

ToLCV-Au.

ICTV decimal code

29.0.3.0.039

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Lycopersicon esculentum - chlorosis and upward curling of leaflet margins, dark stunting.

Transmission

Transmitted by a vector; possible an insect; Bemisia tabaci; Aleyrodidae. Virus not transmitted by mechanical inoculation; transmitted by grafting.

Geographical distribution

Spreads in Australia.

Experimental host range

Few (<3) families susceptible (and limited to graft transmission tests until vector transmission is obtained).

Diagnostically susceptible host species and symptoms

• Lycopersicon esculentum - chlorosis and upward curling of leaflet margins, stunting.
• Datura stramonium - downward curling of leaf margins, interveinal chlorosis, and chlorosis of minor veins.
• Nicotiana benthamiana - leaf malformation, vein chlorosis, prominent veins on abaxial leaf and sepal surfaces.
• Nicotiana tabacum - stunting, chlorosis and upward cupping of leaves.

Diagnostically insusceptible host species

Capsicum annuum, Physalis minima, Physalis peruviana.

Maintenance and propagation hosts

Lycopersicon esculentum, Datura stramonium, Nicotiana benthamiana.

Assay hosts (Local lesions or Whole plants)

Datura stramonium (W); Nicotiana benthamiana (W).

Susceptible host species

• Datura stramonium
• Lycopersicon esculentum
• Nicandra physalodes
• Nicotiana benthamiana
• Nicotiana glutinosa
• Nicotiana tabacum
• Physalis floridana
• Solanum melongena
• Solanum tuberosum

Insusceptible host species

• Capsicum annuum
• Nicotiana rustica
• Physalis minima
• Physalis peruviana

Families containing susceptible hosts

• Solanaceae (9/13)

Families containing insusceptible hosts

• Solanaceae (4/13)

Sources of host-range data

B.D. Conde and J.E. Thomas (unpublished data).

Physical and biochemical properties

Particle morphology

Virions geminate; not enveloped.

Biochemical properties

Genome probably consists of DNA.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• L11746 Em(40)_vi:GETLCAL1R Gb(84)_vi:TLCAL1REP Tomato leaf curl virus AL1 replication protein gene, complete cds. 3/93 294bp.
• L12738 Em(40)_vi:GETLCVSEQ Gb(84)_vi:TLCVSEQ Tomato leaf curl virus coat protein (AR1) gene, partial cds. 4/93 219bp.
• L12739 Em(40)_vi:GETLCVSE1 Gb(84)_vi:TLCVSEQA Tomato leaf curl virus origin of replication. 5/93 165bp.
• S53251 Em(40)_vi:S53251 Gb(84)_vi:S53251 orf V1...orf C4 tomato leaf curl virus TLCV, Australian isolate, Genomic Complete, 6 genes,
• S55329 Em(40)_vi:S55329 Gb(84)_vi:S55329 V1 orf (5´ region) tomato leaf curl virus TLCV, Genomic, 71 nt. 12/93 71bp.
• S55330 Em(40)_vi:S55330 Gb(84)_vi:S55330 V2 orf, C2 orf (3´ regions) tomato leaf curl virus TLCV, Genomic, 51 nt. 12/93 51bp.
• S55334 Em(40)_vi:S55334 Gb(84)_vi:S55334 C2 orf (5´ region) tomato leaf curl virus TLCV, Genomic, 72 nt. 12/93 72bp.
• S55445 Em(40)_vi:S55445 Gb(84)_vi:S55445 C1 orf (5´ region) tomato leaf curl virus TLCV, Genomic, 240 nt. 12/93 240bp
• U15015 Gb(84)n:TLU15015 Tomato leaf curl virus coat protein AV1, AV2, AV3, replication-associated protein AC1, AC2, AC3, AC4, AC5, BV1 and BC1 genes, complete cds.
• U15016 Gb(84)n:TLU15016 Tomato leaf curl virus coat protein AV1, AV2, AV3, replication-associated protein AC1, AC2, AC3, AC4 and AC5 genes, complete cds.
• Z48182 Em(44)n:Tlcvvcgns Gb(90)n:Tlcvvcgns Tomato leaf curl virus V1, V2, C1, C2, C3 and C4 genes. 8/95 2,749bp.

Cytopathology

Virions found in phloem; in nuclei.

Taxonomy and relationships

bigeminivirus: Geminiviridae (based on virion morphology and c-DNA reaction with a probe to African cassava mosaic virus)

Virus(es) with serologically related virions

African cassava mosaic virus.

Additional comments on relationships

Genomic DNA reacts with a c-DNA probe to African cassava mosaic virus (D.J. Robinson, unpublished data).

Comments and References

References

• Aldrick, S. (1970-71). Plant Path. Ann. Rep., Northern Territory Administration (unpublished).
• Thomas, J.E., Massalski, P.R. and Harrison, B.D. (1986). J. gen. Virol. 67: 2739.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.