Plant Viruses
Online 
Descriptions and Lists from
the VIDE Database
Tobacco streak
ilarvirus
Index
Data collated by R.W. Fulton, 1984.
Nomenclature
Synonyms
asparagus stunt virus (Brunt and Paludan, 1970;
Paludan, 1964), datura quercina virus, strawberry necrotic shock virus (Frazier
et al., 1962; Stace-Smith and Frazier, 1971).
Acronym
Strains
bean red node strain (Scott et
al., 1961; Thomas and Zaumeyer, 1950; Virgin, 1943), black raspberry latent
strain (Brunt and Stace-Smith, 1976; Converse and Lister, 1969; Jones and Mayo,
1975).
ICTV decimal code
Host range and symptoms
First reported
in Nicotiana tabacum; from Wisconsin, U.S.A; by Johnson (1936).
Natural host range and symptoms
Symptoms persist (most
hosts do not recover).
- Dahlia spp. - leaf mottling or symptomless.
- Gossypium
herbaceum, Melilotus albus, Trifolium pratense - leaf mottling.
- Phaseolus vulgaris - reddening of nodes.
- Glycine max
- systemic necrosis, bud blight.
- Rosa setigera - leaf vein
yellowing.
- Nicotiana tabacum - systemic necrosis, with recovery.
- Asparagus officinalis - stunting.
Transmission
Transmitted by a vector; an insect;
Frankliniella occidentalis and Thrips tabaci; Thysanoptera
(possibly by allowing virus from the surface of infected pollen to enter through
feeding wounds; Sdoodee and Teakle, 1987). Virus transmitted by mechanical
inoculation; transmitted by grafting; not transmitted by contact between plants;
transmitted by seed (in Chenopodium quinoa, Phaseolus vulgaris, Datura
stramonium to different extents); transmitted by pollen to the pollinated
plant.
Geographical distribution
Probably distributed
worldwide. Spreads in the North American region and the Pacific region;
Australia, Canada, Peru, and the USA (CABI/EPPO data for black raspberry latent
strain).
Experimental host range
Many (>9) families
susceptible.
Diagnostically susceptible host species and
symptoms
- Beta patellaris - necrotic local lesions.
- Cyamopsis tetragonoloba - small dark local lesions.
- Phaseolus vulgaris cv. Manteiga - necrotic local lesions.
- Vigna unguiculata - chlorotic or necrotic local lesions.
- Nicotiana tabacum cv. Turkish - upper leaves become toothed.
Maintenance and propagation hosts
Catharanthus roseus,
Cucumis sativus, Datura stramonium, Nicotiana tabacum.
Assay hosts
(Local lesions or Whole plants)
Beta patellaris (L), Cyamopsis tetragonoloba (L),
Macrotyloma uniflorum (L), Phaseolus vulgaris cv. Manteiga (L),
Vigna unguiculata ssp. cylindrica, (L) for most strains.
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Families containing
insusceptible hosts
Sources of host-range data
Berkeley
and Phillips (1943); Fulton (1948); Kaiser et al. (1982).
Physical and
biochemical properties
Properties of particles in sap
TIP: 64
°C (in sap diluted in buffer containing anti-oxidant). LIV: 1.5 days (most
infectivity lost within 5 min. in crude sap). DEP: log10 minus 1-4 (but
varies over a wide range depending on host species, antioxidants used,
etc.). Electron microscopy: fixation with 1% glutaraldehyde essential.
Purification method
Particle morphology
Virions isometric; not enveloped; 27,
30, and 35 nm in diameter; rounded in profile; without a conspicuous capsomere
arrangement.
Physical properties
Three sedimenting components in
purified preparations; sedimentation coefficient of the fastest 113 S
(B); of the other(s) 90 S (T), or 98 S (M). Density 1.35 g
cm-3 in CsCl. Isoelectric point pH 4.6. A260/A280 ratio 1.56.
Biochemical properties
Virions contain 14 % nucleic acid;
86 % protein; 0 % lipid.
Genome consists of RNA; single-stranded. Total genome size 7.915 kb.
Genome of three parts; largest (or only) genome part the largest 2.94 kb; the
2nd largest 2.77 kb; the 3rd largest 2.205 kb. Genomic nucleic acid isolated by
Van Vloten-Doting (1975). Base composition 25 % G; 25 % A; 20 % C; 30 % U. 5´
terminus of RNA has a methylated nucleotide cap (Van Vloten-Doting, 1981).
Infectivity lost when deproteinised with proteases; lost when deproteinised with
phenol or detergent. Poly A region present (Van Vloten-Doting, 1981). Additional
factor required for infectivity. Genome has no tRNA-like activity.
Sequence database accession code(s)
- J02416
Gb(84)_vi:TSVRNA2 tobacco streak virus rna 2: 3´ end. 9/83 180bp
- J02417
Gb(84)_vi:TSVRNA34 tobacco streak virus rna 3/4 : 3´ end. 9/83 140bp
- V00600 Em(40)_vi:ILTSV1 Gb(84)_vi:ILTSV1 Tobacco streak virus RNA 2 (3´
end). 7/91 180bp.
- V00601 Em(40)_vi:ILTSV2 Gb(84)_vi:ILTSV2 Tobacco streak
virus RNA 3, 3´ end (identical to the 3´ end of RNA 4). 7/91 140bp.
- X00435 Em(40)_vi:TOTSV3 Gb(84)_vi:TOTSV3 Complete sequence of tobacco streak
virus RNA 3 (TSV RNA 3). 9/93 2,205bp. 5 sequences.
Features of the genome
Non-genomic nucleic acid
found in the virions; is subgenomic mRNA; of 0.881 kb. Sub-genomic
mRNA not found in infected cells.
Features of proteins
Virion protein(s) one;
Mr 28500-30000; coat protein. Method of preparation: Van Vloten-Doting
(1975). Amino acid composition: Ghabrial and Lister (1974).
Replication
Replication does not depend on a helper
virus.
Cytopathology
Virions found in all parts of the host
plant; in cytoplasm and in nuclei. Inclusions present in infected cells
(epidermal or guard cells); are unusual in shape; irregularly shaped; they do
not contain virions. Other cellular changes: fibrils in the cytoplasm and
nucleus that are associated with virion aggregates and masses of fibrils in the
cytoplasm that are not associated with virions (Edwardson and Christie, 1986).
Taxonomy and
relationships
Virus(es) with serologically unrelated virions
Apple mosaic, American plum line pattern, asparagus C, citrus leaf
rugose, citrus variegation, elm mottle, lilac ring mottle, prune dwarf, prunus
necrotic ringspot, spinach latent, Tulare apple mosaic viruses.
Comments and
References
References
- Berkeley, G.H. and
Phillips, J.H.H. (1943). Can. J. Res. 21: 181.
- Brunt, A.A. and
Paludan, N. (1970). Phytopath. Z. 69: 277.
- Brunt, A.A. and
Stace-Smith, R. (1976). Acta Hort. 66: 71.
- Converse, R.H. and
Lister, R.M. (1969). Phytopathology 59: 325.
- Edwardson, J.R.
and Christie, R.G. (1986). Fla Agric. Exp. Stn Monog. No. 14, p. 221.
- Frazier, N.W., Jorgensen, P.S., Thomas, H.E. and Johnson, H.A. (1962).
Pl. Dis. Reptr 46: 547.
- Fulton, R.W. (1948).
Phytopathology 38: 421.
- Fulton, R.W. (1967). Virology
32: 153.
- Fulton, R.W. (1970). Virology 41: 288.
- Fulton, R.W. (1971). CMI/AAB Descr. Pl. Viruses No. 44, 4 pp.
- Fulton, R.W. (1985). CMI/AAB Descr. Pl. Viruses No. 307, 5 pp.
- Ghabrial, S.A. and Lister, R.M. (1974). Virology 57: 1.
- Jones, A.T. and Mayo, M.A. (1975). Ann. appl. Biol. 79: 297.
- Johnson, J. (1936). Phytopathology 26: 285.
- Kaiser, W.J.,
Wyatt, S.D. and Pesho, G.R. (1982). Phytopathology 72: 1508.
- Lister, R.M. and Saksena, K.N. (1976). Virology 70: 440.
- Lister, R.M., Ghabrial, S.A. and Saksena, K.N. (1972). Virology
49: 290.
- Paludan, N. (1964). Maanedsovers. PlSygd. 407:
11.
- Scott, H.A., Vincint, M. and Zaumeyer, W.J. (1961).
Phytopathology 51: 755.
- Sdoodee, R. and Teakle, D.S. (1987).
Plant Path. 36: 377.
- Stace-Smith, R. and Frazier, N.W. (1971).
Phytopathology 61: 757.
- Thomas, H.R. and Zaumeyer, W.J.
(1950). Phytopathology 40: 832.
- Van Vloten-Doting, L. (1975).
Virology 65: 215.
- Van Vloten-Doting, L. (1981).
Intervirology 15: 198.
- Virgin, W.J. (1943).
Phytopathology 33: 743.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
(1996 onwards).
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
http://biology.anu.edu.au/Groups/MES/vide/
Dallwitz (1980)
and
Dallwitz, Paine and Zurcher (1993)
should also be cited.
Please send comments, corrections and suggestions to:
vide-manager@biology.anu.edu.au