Plant Viruses
Online 
Descriptions and Lists from
the VIDE Database
Tobacco etch
potyvirus
Index
Data collated by D.E. Purcifull, 1981.
Nomenclature
Synonyms
datura Z virus, tobacco severe etch virus, tomato
etch virus.
Acronym
ICTV decimal code
Host range and symptoms
First reported
in Datura spp.; from the U.S.A; by Blakeslee (1921).
Natural host range and symptoms
Symptoms persist (may be
strain-dependant).
- Cassia obtusifolia - leaf mottling, malformation and
necrosis, stunting and reduced seed yield.
- Capsicum annuum -
mottling, mosaic and leaf malformation, fruit malformation, stunting.
- Datura stramonium - leaf mottling, vein banding and malformation,
suppression of fruit spines.
- Lycopersicon esculentum - leaf
mottling and distortion, severe yield reductions.
- Nicotiana tabacum
- mottling and necrotic leaf etching.
- Physalis spp. - leaf
chlorosis.
Transmission
Transmitted by a vector; an insect; more
than 10 spp., especially Myzus persicae; Aphididae. Transmitted in a
non-persistent manner. Virus does not require a helper virus for vector
transmission (Simons, 1976; Pirone, 1979); transmitted by mechanical
inoculation; not transmitted by seed.
Geographical
distribution
Spreads in the Eastern Asian region and the South and
Central American region; Canada, China, Mexico, Puerto Rico, Venezuela, and the
USA.
Experimental host range
Many (>9) families
susceptible.
Diagnostically susceptible host species and
symptoms
- Cassia obtusifolia - necrotic local lesions;
systemic mosaic and leaf malformation.
- Capsicum annuum - systemic
mosaic, rugosity and leaf malformation.
- C. frutescens - root
necrosis, wilting and death.
- Chenopodium album, C. amaranticolor and
C. quinoa - local chlorotic or necrotic lesions; not systemic.
- Datura stramonium - systemic mosaic, ringspots, rugosity and leaf
malformation.
- Nicotiana tabacum - chlorotic local lesions;
systemic mosaic, ringspots, leaf malformation and necrotic etching.
Maintenance and propagation hosts
Capsicum annuum,
Datura stramonium, Nicotiana tabacum.
Assay hosts (Local lesions or Whole plants)
Chenopodium amaranticolor (L), C. quinoa (L).
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Families containing
insusceptible hosts
Sources of host-range data
Holmes
(1946); Schmelzer (1967); Edwardson (1974).
Physical and
biochemical properties
Properties of particles in sap
TIP: 55
°C. LIV: 5-10 days. DEP: log10 minus 4. Leaf sap contains few virions.
Purification method
Dougherty and
Hiebert (1980).
Particle morphology
Virions filamentous; not enveloped;
usually flexuous; with a clear modal length; of 730-750 nm; 12-13 nm wide. Basic
helix obvious (in UF).
Physical properties
One sedimenting component in purified
preparations; sedimentation coefficient 154 S. Density 1.33 g cm-3
in CsCl.
Biochemical properties
Virions contain 5 % nucleic acid;
95 % protein; 0 % lipid.
Genome consists of RNA; single-stranded. Total genome size 9.494 kb.
Genome unipartite; largest (or only) genome part 9.494 kb. Base composition 23 %
G; 30 % A; 20 % C; 27 % U. 5´ terminus of RNA has a VPg. Infectivity retained
when deproteinised with proteases, or decreased when deproteinised with
proteases. Poly A region present; at the 3´ terminus.
Sequence database accession code(s)
- M11216
Em(40)_vi:TEVCAPG Gb(84)_vi:TEVCAPG Tobacco etch virus capsid protein gene. 4/90
2,324bp.
- M11458 Em(40)_vi:TECGHAT Gb(84)_vi:TEVCGHAT Tobacco etch virus
(highly aphid transmissible (HAT)) complete genome. 4/90 9,494bp.
- M15239
Em(40)_vi:TEVGEN Gb(84)_vi:TEVGEN Tobacco etch virus RNA, complete genome. 7/89
9,497bp.
- L38714 Em(43)_vi:Tevnwp Gb(89)_vi:Tevnwp Tobacco etch virus P1,
Helper Component and Proteinase, P3, Cylindric Inclusion, Nuclear Inclusion.
Features of the genome
Sub-genomic mRNA not
found in infected cells.
Features of proteins
Virion protein(s) one;
Mr 30000-32000; coat protein. Method of preparation: Hiebert and
McDonald (1973); Hiebert et al. (1979).
Replication
Replication does not depend on a helper
virus.
Cytopathology
Virions found in mesophyll and epidermis;
in cytoplasm; plasmodesmata. Inclusions present in infected cells; are crystals
in the nucleus and pinwheels; they do not contain virions.
Taxonomy and
relationships
Virus(es) with serologically related virions
Potato Y and 13 other viruses (Shepard et al., 1974).
Comments and
References
References
- Blakeslee, A.F. (1921).
J. Genet. 11: 17.
- Christie, R.G. and Edwardson, J.R. (1977).
Fla Agric. Exp. Stn Monog. No. 9, p. 55.
- Damirdagh, I.S. and
Shepherd, R.J. (1970). Virology 40: 84.
- Dougherty, W.G. and
Hiebert, E. (1980). Virology 101: 466.
- Dougherty, W.G. and
Hiebert, E. (1980a). Virology 104: 183.
- Dougherty, W.G.
and Hiebert, E. (1980b). Virology 104: 174.
- Edwardson,
J.R. (1968). Virology 34: 250.
- Edwardson, J.R. (1974). Fla
Agric. Exp. Stn Monog. No. 4, p. 398.
- Hari, V. (1981). Virology
112: 391.
- Hiebert, E. and McDonald, J.G. (1973). Virology
56: 349.
- Hiebert, E., Tremaine, J.H. and Ronald, W. (1979).
Phytopathology 69: 1031.
- Hill, J.H. and Benner, H.I. (1976).
Virology 75: 419.
- Holmes, F.O. (1946). Phytopathology
36: 643.
- Jones, A.T. and Barker, H. (1976). Ann. appl. Biol.
83: 231.
- Knuhtsen, H., Hiebert, E. and Purcifull, D.E. (1974).
Virology 61: 200.
- Pirone, T.P. (1979). Phytopathology
69: 531.
- Purcifull, D.E. (1973). Virology 55: 275.
- Purcifull, D.E. and Batchelor, D.L. (1977). Bull. Univ. Fla Agric. Exp.
Stn 788: 39 pp.
- Purcifull, D.E. and Hiebert, E. (1982).
CMI/AAB Descr. Pl. Viruses No. 258, 7 pp.
- Shepard, J.E., Secor, G.A.
and Purcifull, D.E. (1974). Virology 58: 464.
- Shepherd, R.J.
and Purcifull, D.E. (1972). CMI/AAB Descr. Pl. Viruses No. 55, 4 pp.
- Schmelzer, K. (1967). Phytopath. Z. 60: 301.
- Simons, J.N.
(1976). Phytopathology 66: 652.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
(1996 onwards).
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
http://biology.anu.edu.au/Groups/MES/vide/
Dallwitz (1980)
and
Dallwitz, Paine and Zurcher (1993)
should also be cited.
Please send comments, corrections and suggestions to:
vide-manager@biology.anu.edu.au