Plant Viruses
Online 
Descriptions and Lists from
the VIDE Database
Rubus Chinese
seed-borne (?) nepovirus
Index
Data collated by D.J. Barbara, 1986.
Nomenclature
Acronym
ICTV decimal code
Host range and symptoms
First reported
in Rubus spp.; from the U.K. (in seed imported from China); by Barbara
et al. (1985).
Natural host range and symptoms
- Rubus spp.
- infected seedlings show no symptoms.
Transmission
As the virus is probably a nepovirus, it is
likely to have a nematode vector. Virus transmitted by mechanical inoculation;
transmitted by seed.
Geographical distribution
Spreads in
China. Found, but with no evidence of spread, in the U.K. from seed imported
from China.
Experimental host range
Several (3-9) families
susceptible.
Diagnostically susceptible host species and
symptoms
- Chenopodium quinoa - chlorotic local lesions,
systemic chlorosis, deformation, necrotic flecking.
- Cucumis sativus
- chlorotic local lesions, systemic chlorotic flecking or chlorosis.
Maintenance and propagation hosts
Nicotiana clevelandii,
Chenopodium quinoa.
Assay hosts (Local
lesions or Whole plants)
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Families containing
insusceptible hosts
Sources of host-range data
Physical and
biochemical properties
Properties of particles in sap
LIV: 1-2
days. DEP: log10 minus 4-5. Leaf sap contains few virions. Electron
microscopy: stable in 2% Na-PTA, pH 6.5, partially disrupted in 2% UA.
Purification method
Particle morphology
Virions isometric; not enveloped; 30
nm in diameter (some virions are penetrated by negative stain and these are
8-10% smaller in some preparations); angular in profile; without a conspicuous
capsomere arrangement.
Physical properties
Two sedimenting components in
purified preparations (in sucrose density gradients), or three sedimenting
components in purified preparations (in CsCl). Density 1.461 g cm-3 in
CsCl, or 1.452 g cm-3 in CsCl (not fixed with aldehydes). A260/A280
ratio 1.71 (B), or 0.87 (T).
Biochemical properties
Virions contain 37 % nucleic acid
(B), or 36 % nucleic acid (M), or 0 % nucleic acid (T); 63 % protein (B), or 64
% protein (T), or 100 % protein (T).
Genome consists of RNA; single-stranded; possibly linear; of two parts.
Genomic nucleic acid isolated by Barbara et al. (1985).
Features of the genome
Non-genomic nucleic acid
not found in the virions.
Features of proteins
Virion protein(s) two;
Mr of the larger 47000; probably structural protein. Mr of
2nd largest 25200; structural protein. Method of preparation: Barbara et
al. (1985).
Replication
Replication does not depend on a helper
virus.
Cytopathology
Virions found in mesophyll and epidermis
(so far only these have been tested); in cytoplasm. Inclusions present in
infected cells; are membranous bodies and unusual in shape; and tubular
structures within the outer membrane; they contain virions.
Taxonomy and
relationships
Virus(es) with serologically related virions
Strawberry latent ringspot virus.
Virus(es) with
serologically unrelated virions
Arabis mosaic,
cherry leaf roll, elm mosaic, myrobalan latent ringspot, satsuma dwarf, tobacco
ringspot, tomato black ring, and tomato ringspot viruses.
Best tests for diagnosis
Comments and
References
References
- Barbara, D.J., Ashby,
S.C. and McNamara, D.G. (1985). Ann. appl. Biol. 107: 45.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
(1996 onwards).
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
http://biology.anu.edu.au/Groups/MES/vide/
Dallwitz (1980)
and
Dallwitz, Paine and Zurcher (1993)
should also be cited.
Please send comments, corrections and suggestions to:
vide-manager@biology.anu.edu.au