Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Rice tungro bacilliform badnavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by H. Hibino, 1987. Updated by A.A. Brunt, 1991.

Nomenclature

Synonyms

rice yellow orange leaf virus.

Acronym

RTBV

ICTV decimal code

07.0.1.0.009

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Oryza sativa - leaves yellow-orange, stunting.

Transmission

Transmitted by a vector; an insect; especially Nephotettix virescens, but also N. nigropictus, N. cincticeps, N. malayanus, N. parvus, Recilia dorsalis; Cicadellidae. Transmitted in a semi-persistent manner. Virus lost by the vector when it moults; does not multiply in the vector; not transmitted congenitally to the progeny of the vector; requires, for vector transmission, a helper virus (rice tungro spherical waikavirus); not transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Ecology and control

Studies reported by Cabunagan et al. (1987); Hibino et al. (1987); Hibino et al. (1988); Tiongco et al. (1988); Khan et al. (1991).

Geographical distribution

Spreads in the Eastern Asian region; China.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Oryza sativa - leaves yellow-orange, stunting.

Maintenance and propagation hosts

Oryza sativa.

Assay hosts (Local lesions or Whole plants)

Oryza sativa (W), Oryza australiensis (W), Oryza longistaminata (W), Oryza barthii (W), Oryza latifolia (W), Oryza nivara (W), Oryza glaberrima (W), Oryza perennis (W), Eleusine indica (W), Cyperus rotundus (W), Fimbristylis miliacea (W), Leersia hexandra (W), Monochoria vaginalis (W).

Susceptible host species

• Cyperus rotundus
• Eleusine indica
• Fimbristylis miliacea
• Leersia hexandra
• Monochoria vaginalis
• Oryza australiensis
• Oryza barthii
• Oryza glaberrima
• Oryza latifolia
• Oryza longistaminata
• Oryza nivara
• Oryza perennis
• Oryza sativa

Families containing susceptible hosts

• Cyperaceae (2/2)
• Gramineae (10/10)
• Pontederiaceae (1/1)

Sources of host-range data

Anjaneyulu et al. (1988).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions. Electron microscopy: use PTA or UA, fixation not required.

Purification method

Omura et al. (1983); Cabauatan and Hibino (1988).

Particle morphology

Virions bacilliform; not enveloped; 110-400 nm in length; 30-35 nm wide.

Physical properties

One sedimenting component in purified preparations. Density 1.312 g cm^-3 in CsCl.

Biochemical properties

Genome consists of DNA; double-stranded; circular; unipartite; largest (or only) genome part 8.3 kb. Genomic nucleic acid isolated by Hibino et al. (1991).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D10774 Em(40)_vi:RTBWG Gb(84)_vi:RTBWG Rice tungro bacilliform virus whole genome. 9/92 8,002bp.
• M65026 Em(40)_vi:LERTUORFS Gb(84)_vi:RTUORFS Rice tungro bacilliform open reading frames 1, 2, 3, and 4 genomic RNA. 12/92 8,000bp.
• M95497 Em(40)_vi:LERTUPOLY Gb(84)_vi:RTUPOLYP Rice tungro virus polyprotein gene, complete cds. 1/93 12,484bp.
• X57924 Em(40)_vi:RTBVPHIL Gb(84)_vi:RTBVPHIL Rice tungro bacilliform virus (infectious clone) ORF's P24, P12, P194 & P46. 6/91 8,002bp. 4 sequences.

Features of proteins

Virion protein(s) one; M_r 32000; coat protein.

Cytopathology

Virions found in leaves, roots, vascular parenchyma and phloem; in cytoplasm. Inclusions absent from infected cells. Other cellular changes: starch accumulation.

Taxonomy and relationships

Badnavirus

Best tests for diagnosis

ELISA or latex serology, transmission test using Nephotettix virescens, symptomatology.

Comments and References

General comments

Rice "tungro" disease is caused by a complex of rice tungro badnavirus and rice tungro spherical waikavirus; the badnavirus depends on the waikavirus for its transmission by leafhoppers, and causes the tungro symptoms, whereas the waikavirus alone causes mild stunting and enhances the tungro symptoms.

References

• Anjaneyulu, A., Daquioag, R.D., Mesina, M. E., Hibino, H., Lubigan, R.T. and Moody, K. (1988). Int. Rice Res. Newsl. 13: 30.
• Bajet, N.B., Daquioag, R.D. and Hibino, H. (1985). J. Pl. Prot. Tropics 2: 125.
• Bao, Y. and Hull, R. (1992). J. gen. Virol. 73: 1297.
• Cabauatan, P.Q. and Hibino, H. (1985). Philipp. Phytopathol. 21: 103.
• Cabauatan, L.Q. and Hibino, H. (1988). Plant Dis. 72: 525.
• Cabunagan, R.C., Flores, Z.M., Hibino, H., Elazegui, F. and Mew, T.W. (1987). Int. Rice Res. Newsl. 12: 25.
• Favali, M.A., Pellegrini, S. and Bassi, M. (1975). Virology 66: 793.
• Hay, M., Jones, M.C., Blakebrough, M.L., Dasgupta, I., Davies, J.W. and Hull, R. (1991). Nucl. Acids Res. 19: 2615.
• Hibino, H., Roechan, M. and Sudarisman, S. (1978). Phytopathology 68: 1412.
• Hibino, H., Saleh, N. and Roechan, M. (1979). Phytopathology 69: 1266.
• Hibino, H. (1983). Plant Dis. 17: 774.
• Hibino, H. (1983). Ann. Phytopath. Soc. Japan 49: 545.
• Hibino, H. and Cabauatan, P.Q. (1986). Phytopathology 77: 473.
• Hibino, H. and Cabunagan, R.C. (1986). Tropical Agric. Res. Series 19: 173.
• Hibino, H., Ishikawa, K., Omura, T., Cabauatan, P.Q. and Koganezawa, H. (1991). Phytopathology 81: 1130.
• Hibino, H., Tiongco, E.R., Cabunagan, R.C. and Flores, Z.M. (1987). Phytopathology 77: 871.
• Khan, M.A., Hibino, H., Aguiero, V.M., Daquioag, R.D. and Opina, O.S. (1919). Plant Dis. 75: 926.
• Ling, K.C. (1966). Phytopathology 60: 795.
• Omura, T., Saito, Y., Usugi, T. and Hibino, H. (1982). Ann. Phytopath. Soc. Japan 49: 73.
• Omura, T., Hibino, H. and Usugi, T. (1984). Plant Dis. 68: 374.
• Qu, R., Bhattacharya, M., Laco, G.S., Subba Rao, B.L., Kaniewska, M.B., Elmer, J.S., Rochester, D.E., Smith, C.E. and Beachy, R.N. (1991). Virology 185: 354.
• Rivera, C.T. and Ou, S.H. (1965). Pl. Dis. Reptr 49: 127.
• Saito, Y. (1977). Tropical Agric. Res. Series 10: 129.
• Saito, Y., Roechan, M., Tantera, D.M. and Iwaki, M. (1975). Phytopathology 65: 793.
• Tiongco, E.R., Cabunagan, R.C., Flores, Z.M. and Hibino, H. (1988). Int. Rice Res. Newsl. 13: 10.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.