Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Rice stripe tenuivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Toriyama, 1984. Revised 1989.

Nomenclature

Synonyms

ine shima-ha gare uirusu.

Acronym

RSV

ICTV decimal code

69.0.1.0.005

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Avena sativa, Digitaria adscendens, D. violascens, Eragrostis multiflorum, Hordeum vulgare, Oryza sativa, Setaria italica, S. viridis, Triticum aestivum, Zea mays - systemic necrotic or chlorotic spots and streaking.

Transmission

Transmitted by a vector; an insect; Laodelphax striatellus, Terthron albovittatum, Unkanodes sapporona, U. albifascia; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; transmitted congenitally to the progeny of the vector; transmitted by mechanical inoculation (but only with great difficulty); not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in the Eastern Asian region; China, Japan, Taiwan, and the former USSR.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Oryza sativa (Japonica type), Triticum aestivum, Zea mays - chlorotic stripe and pale streaking.

Diagnostically insusceptible host species

Anthoxanthum odoratum, Elymus tsukushiensis var. transiens, Festuca arundinacea, Phalaris arundinacea, Poa pratensis.

Maintenance and propagation hosts

Oryza sativa Japonica type varieties, Triticum aestivum, Zea mays.

Assay hosts (Local lesions or Whole plants)

Oryza sativa japonica type cvs Norin 8, Kinmaze, Triticum aestivum, Zea mays cv. Golden Cross Bantam, all (W).

Susceptible host species

• Avena sativa
• Cynodon dactylon
• Dactylis glomerata
• Digitaria adscendens
• Digitaria violascens
• Eragrostis multiflorum
• Hordeum vulgare
• Lolium perenne
• Oryza sativa
• Oryza sativa var. japonica
• Phleum pratense
• Secale cereale
• Setaria italica
• Setaria viridis
• Triticum aestivum
• Zea mays

Insusceptible host species

• Anthoxanthum odoratum
• Echinochloa crus-galli
• Elymus tsukushiensis var. transiens
• Festuca arundinacea
• Phalaris arundinacea
• Poa pratensis

Families containing susceptible hosts

• Gramineae (16/22)

Families containing insusceptible hosts

• Gramineae (6/22)

Sources of host-range data

Iida (1969); Toriyama (1983a).

Physical and biochemical properties

Properties of particles in sap

TIP: 50-55 °C. LIV: 4 days in extracts of viruliferous insects kept at 4ºC, 8-12 months in viruliferous insects and diseased rice plants at -20ºC and 1-2 months in purified preparations at -20ºC. DEP: log₁₀ minus 3-4 (from plants, 10^-4 to 10^-5 from viruliferous insects). Leaf sap contains many virions.

Purification method

Toriyama (1982a).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; 8 nm wide. Axial canal obvious (canal- like structures are seen in some virions). Basic helix obvious.

Physical properties

Four sedimenting components in purified preparations (T, M₁, M₂, B and nB); sedimentation coefficient 98 S (nB); of the other(s) 68 S (M), or 80 S (B). Density 1.282 g cm^-3 in CsCl (not fixed with aldehydes). Isoelectric point pH 4.5.

Biochemical properties

Virions contain 12 % nucleic acid; 88 % protein.

Genome consists of RNA; single-stranded; linear; of four parts; largest (or only) genome part 9 kb; the 2nd largest 3.5 kb; the 3rd largest 2.48 kb; the 4th largest 2.14 kb. Genomic nucleic acid isolated by Toriyama (1982a, 1982b, 1989).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D01039 Em(40)_vi:RCSSEG4 Gb(84)_vi:RISSEG4 Rice stripe virus genome, segment4, complete sequence. 12/92 2,137bp.
• D01094 Em(40)_vi:RCSRSVAS3 Gb(84)_vi:RSTRSVAS3 Rice stripe virus (RSV) ambisense genes in segment 3. 8/92 2,475bp.
• D10979 Em(40)_vi:RCSRNA41 Gb(84)_vi:RCSRNA4 Rice stripe virus RNA 4, complete sequence. 9/92 2,157bp
• D13787 Gb(84)n:RCSRNA2 Rice stripe virus segment 2 RNA for ORF 1,2. 9/94 3,514bp.
• D13176 Em(43)_vi:Rcsrna Gb(89)_st:Rcsrna Rice stripe virus RNA. 9/94 3,514bp.
• D31879 Em(43)_vi:Rcsrnap Gb(89)_vi:Rcsrnap Rice stripe virus RNA for polymerase. 1/95 8,970bp.
• X53563 Em(40)_vi:RSVRNA3 Gb(84)_vi:RSVRNA3 Rice stripe virus RNA3 genomic RNA for coat protein (two ORF's). 9/91 2,504bp.

Features of the genome

Features of the genome: Purified rice stripe virus preparation has a RNA-dependent RNA polymerase.

Features of proteins

Virion protein(s) two; M_r of the larger 32000. M_r of 2nd largest 230000. Amino acid composition: Toriyama (1983).

Cytopathology

Virions found in epidermis; in nuclei (rarely), or in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm and amorphous X-bodies; they do not contain virions.

Taxonomy and relationships

Tenuivirus

Virus(es) with serologically related virions

Maize stripe tenuivirus, also possibly rice grassy stunt tenuivirus.

Comments and References

References

• Iida, T.T. (1969). In: The Virus Diseases of the Rice Plant, p. 3. John Hopkins, Baltimore.
• Kakutani, T., Hayano, Y., Hayashi, T. and Minobe, Y. (1990). J. gen. Virol. 71: 1427.
• Kakutani, T., Hayano, Y., Hayashi, T. and Minobe, Y. (1991). J. gen. Virol. 72: 465.
• Kishimoto, R. (1967). Virology 32: 144.
• Kiso, A. and Yamamoto, T. (1973). Rev. Pl. Prot. Res. 6: 75.
• Koganezawa, H., Doi, Y. and Yora, K. (1975). Ann. Phytopath. Soc. Japan 41: 148.
• Kuribayashi, K. (1931). Bull. Nagano Agr. Exp. Stn 2: 45.
• Shinkai, A. (1962). Bull. Natl. Inst. Agr. Sci. 14: 1.
• Takahashi, M., Toriyama, S., Kikuchi, Y., Hayakawa, T. and Ishihama, A. (1990). J. gen. Virol. 71: 2817.
• Takahashi, M., Toriyama, S., Hamamatsu, C. and Ishihama, A. (1993). J. gen. Virol. 74: 769.
• Toriyama, S. (1987). J. gen. Virol. 68: 925.
• Toriyama, S. (1982a). J. gen. Virol. 61: 187.
• Toriyama, S. (1982b). Ann. Phytopath. Soc. Japan 48: 482.
• Toriyama, S. (1983a). CMI/AAB Descr. Pl. Viruses No. 269, 5 pp.
• Toriyama, S. (1986). J. gen. Virol. 67: 1247.
• Toriyama, S. (1986). Microbiol. Sciences 3: 347.
• Toriyama, S. and Watanabe, Y. (1989). J. gen. Virol. 70: 505.
• Zhu, Y., Hayakawa, T., Toriyama, S. and Takahashi, M. (1992). J. gen. Virol. 72: 763.
• Zhu, Y., Hayakawa, T. and Toriyama, S. (1992). J. gen. Virol. 73: 1309.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.