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Descriptions and Lists from the VIDE Database

Rice black-streaked dwarf fijivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by E. Shikata, 1987; Li Jing, 1987.

Nomenclature

Synonyms

rice black streak virus.

Acronym

RBSDV

ICTV decimal code

60.0.7.2.003

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Oryza sativa, Zea mays, Triticum sativum, Avena sativa, Hordeum vulgare - stunted, deformed leaves with white waxy or black-streaked swellings along the veins.

Transmission

Transmitted by a vector; an insect; Laodelphax striatellus (Kuribayashi and Shinkai, 1952; Shinkai, 1962), Unkanodes sapporona (Shinkai, 1966) and U. albifascia (Shinkai, 1967); Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation; not transmitted by seed.

Geographical distribution

Spreads in China, Japan, Korea D.P.R. (North), and Korea Republic.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Oryza sativa var. japonica - stunting, darkening of leaves, twisting of tips of young leaves, white waxy swellings along veins on the abaxial leaf surface, later becoming brown and forming black-streaked tumours.
• Zea mays - dwarfening, darkening of leaves, white streaks along the veins and white waxy swollen veins on the abaxial surface of leaves.
• Triticum sativum, Avena sativa, Hordeum vulgare - severe stunting, twisting of leaves, sometimes with waxy swollen veins on the abaxial surface of leaves and culms.

Maintenance and propagation hosts

Oryza sativa, Triticum sativum, Avena sativa and Hordeum vulgare.

Assay hosts (Local lesions or Whole plants)

Zea mays cv. Golden Cross Bantam (W) (Zea mays useless as a virus source for insect transmission).

Susceptible host species

• Avena sativa
• Hordeum vulgare
• Oryza sativa
• Oryza sativa var. japonica
• Triticum aestivum
• Triticum sativum
• Zea mays

Families containing susceptible hosts

• Gramineae (7/7)

Sources of host-range data

Shinkai (1962); Morinaka and Sakurai (1968).

Physical and biochemical properties

Properties of particles in sap

TIP: 60 °C. LIV: 6 days, or 7 days. DEP: log₁₀ minus 5 (in rice plants), or 6 (in insects). Leaf sap contains many virions. Electron microscopy: leaves fixed in 2% osmium tetroxide or 2% paraformaldehyde before extraction yield the large virions of 75-80 nm with the outer projections attached.

Purification method

Kitagawa and Shikata (1969); Kawano et al. (1984).

Particle morphology

Virions isometric; not enveloped; 75-80 nm in diameter (in cells of infected plants and insects), or 55 nm in diameter (B-spiked subvirions), or 70 nm in diameter (intact virions); angular in profile; without a conspicuous capsomere arrangement.

Biochemical properties

Genome consists of RNA; double-stranded. Total genome size 23.898 kb. Genome of 10 parts (named S1-10); largest (or only) genome part 3.998 kb; the 2nd largest 3.385 kb; the 3rd largest 3.31 kb; the 4th largest 3.31 kb; the 5th largest 3.1 kb; the 6th largest and other parts 2.45 kb (pairs; also 2.198, 1.830, 1.826 and 1.810 kb pairs).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00606 Em(40)_vi:RBDSEG10 Gb(84)_vi:RBDSEG10 Rice black-streaked dwarf virus genome segment 10. 11/90 1,801bp.
• L36524 Gb(84)n:RBDGS3A Rice black-streaked dwarf virus genome segment 3 fragment. 10/94 939bp.
• S63914 Em(40)_vi:S63914 Gb(84)_vi:S63914 orf (segment 8) rice black-streaked dwarf virus RBSDV, Genomic RNA, 1927 nt. 1/94 1,927bp.
• S63917 Em(40)_vi:S63917 Gb(84)_vi:S63917 orf1, orf2 (segment 7) rice black-streaked dwarf virus RBSDV, Genomic RNA, 2193 nt. 1/94 2.

Cytopathology

Virions found in phloem; in cytoplasm. Inclusions present in infected cells; are viroplasms, or crystals in the nucleus; they contain virions. Other cellular changes: hyperplasia and hypertrophy of phloem cells.

Taxonomy and relationships

Fijivirus: Reoviridae

Additional comments on relationships

Reported to be serologically closely related to maize rough dwarf fijivirus (Luisoni et al., 1973); their relationship is so close that they may be the same species.

Comments and References

References

• Kashiwagi, Y. (1966). Ann. Phytopath. Soc. Japan 32: 168.
• Kawano, S., Uyeda, I. and Shikata, E. (1984). J. Fac. Agric. Hokkaido Univ. 61: 408.
• Kitagawa, Y. and Shikata, E. (1969). Mem. Fac. Agric. Hokkaido Univ. 6: 446.
• Kuribayashi, K. and Shinkai, A. (1952). Ann. Phytopath. Soc. Japan 16: 41.
• Luisoni, E., Lovisolo, O., Kitagawa, Y. and Shikata, E. (1973). Virology 52: 281.
• Morinaka, T. and Sakurai, Y. (1968). Bull. Chugoku agric. exp. Stn. Ser. E 2: 1.
• Shikata, E. (1974). CMI/AAB Descr. Pl. Viruses No. 135, 4 pp.
• Shinkai, A. (1962). Bull. nat. Inst. agric. Sci., Tokyo Ser. C., p. 223.
• Shinkai, A. (1966). Ann. Phytopath. Soc. Japan 32: 317.
• Shinkai, A. (1967). Ann. Phytopath. Soc. Japan 35: 318.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.