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Descriptions and Lists from the VIDE Database

Raspberry ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt, 1992.

Nomenclature

Synonyms

raspberry Scottish leaf curl virus, red currant ringspot virus.

Acronym

RpRSV

Strains

type strain, Lloyd George yellow blotch strain, English strain.

ICTV decimal code

18.0.3.0.026

Host range and symptoms

Natural host range and symptoms

• Rubus idaeus cv. Norfolk Giant - leaf curling, dieback and death (cv. Lloyd George is immune), but less conspicuous symptoms in other cultivars.
• Fragaria vesca -dwarfing and eventually death.
• Narcissus pseudonarcissus - symptomless infection.
• The virus may infect species of wild and cultivated dicotyledonous and monocotyledonous plants.

Transmission

Transmitted by a vector; a nematode; Longidorus elongatus, L. macrosoma; Dorylamidae. Virus transmitted by mechanical inoculation; transmitted by seed; transmitted by pollen to the seed.

Ecology and control

Studies reported by Cadman (1956); Murant et al. (1968); Kleinhempel (1970); Murant (1970); Bovey et al. (1972); Jennings (1964).

Geographical distribution

Spreads in the Eurasian region and the Mediterranean region; Austria, Belgium, Bulgaria, the former Czechoslovakia, Finland, France, Germany, Greece, Hungary, Ireland, Luxembourg, the Netherlands, Norway, Poland, Spain, Switzerland, Turkey, the UK, the USA, the former USSR, the former Yugoslavia. Found, but with no evidence of spread, in Denmark.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - chlorotic or necrotic local lesions; no systemic infection.
• C. quinoa - chlorotic or necrotic local lesions; systemic mottling and apical necrosis.
• Nicotiana rustica - local lesions or rings; systemic ring and line patterns but systemic infection later often symptomless.
• Phaseolus vulgaris - necrotic local lesions (in winter) and chlorotic local lesions (in summer); systemic mottling, distortion and necrosis.
• Petunia × hybrida - local chlorotic lesions; systemic chlorosis, line patterns and/or necrosis.

Maintenance and propagation hosts

Nicotiana clevelandii, N. rustica, Petunia × hybrida.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L/W), Spinacia oleracea (W, especially useful as a bait plant for vector transmission tests).

Susceptible host species

• Beta vulgaris
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucumis sativus
• Datura stramonium
• Fragaria vesca
• Lycopersicon esculentum
• Narcissus pseudonarcissus
• Nicotiana clevelandii
• Nicotiana rustica
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Rubus idaeus
• Spinacia oleracea
• Vigna unguiculata

Families containing susceptible hosts

• Amaryllidaceae (1/1)
• Chenopodiaceae (4/4)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (2/2)
• Rosaceae (2/2)
• Solanaceae (6/6)

Physical and biochemical properties

Properties of particles in sap

TIP: 70 °C. LIV: 21 days. DEP: log₁₀ minus 4.

Particle morphology

Virions isometric; 30 nm in diameter; angular in profile.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient 125 S (B); of the other(s) 91 S (M), or 50 S (T). A₂₆₀/A₂₈₀ ratio 1.78 (B), or 1.62 (M).

Biochemical properties

Virions contain 43 % nucleic acid (B), or 29 % nucleic acid (M), or 0 % nucleic acid (T); 57 % protein (B), or 71 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded. Total genome size 12.727 kb. Genome of two parts; largest (or only) genome part 8.485 kb; the 2nd largest 4.242 kb. Nucleotide sequence references: Mayo et al. (1992).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• S46011 Em(40)_un:S46011 Gb(84)_vi:S46011 polyprotein raspberry ringspot virus RRV, Genomic RNA Complete, 3928 nt. 2/93 3,928bp. 1 sequence.

Features of proteins

Virion protein(s) one; M_r 54000; coat protein.

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, strawberry latent ringspot, sweet potato ringspot, tobacco ringspot, tomato black ring and tomato ringspot viruses.

Differences between type strain and others

Many variants occur. Cultivars differ in susceptibility; Norfolk Giant is very susceptible to the type strain, but Lloyd George is immune to it.

Comments and References

References

• Bovey, R. et al. (1972). In: La Défense des Plantes Cultivées 6th Edition. Payot, Switzerland.
• Cadman, C.H. (1956). J. Hort. Sci. 31: 111.
• Cadman, C.H. (1960). Virology 11: 653.
• Cropley, R. (1961). Ann. appl. Biol. 49: 530.
• Hanada, K. and Harrison, B.D. (1977). Ann. appl. Biol. 85: 79.
• Harrison, B.D. (1958). Ann. appl. Biol. 46: 571.
• Harrison, B.D. (1961). Tijdschr. PlZiekt. 67: 562.
• Harrison, B.D. (1964). Virology 22: 544.
• Kleinhempel, H. (1970). Archiv. Gartenbau. 18: 267.
• Lister, R.M. and Murant, A.F. (1967). Ann. appl. Biol. 59: 49.
• Maat, D.Z., van der Meer, F.A. and Pfaeltzer (1962). Tijdschr. PlZiekt. 68: 120.
• Mayo, M.A., Acosta, O., Blok, V.C., Wardell, I.M., Manoukian, A., Jolly, C.A. and Robinson, D.J. (1992). Rep. Scottish Crop Res. Inst. 1991, p. 84.
• Murant, A.F. (1970). In: Virus Diseases of Small Fruits and Grapevines, pp. 132-148; ed. N.W. Frazier, University of California, Berkeley, California, U.S.A.
• Murant, A.F. (1978). CMI/AAB Descr. Pl. Viruses No. 198, 5 pp.
• Murant, A.F. (1981). In: Handbook of Plant Virus Infections: Comparative Diagnosis, pp. 197-238; ed. E. Kurstak, Elsevier/North Holland Press, Amsterdam.
• Murant, A.F. and Taylor, M. (1978). J. gen. Virol. 41: 53.
• Murant, A.F. and Lister, R.M. (1967). Ann. appl. Biol. 59: 63.
• Murant, A.F., Taylor, C.E. and Chambers, J. (1968). Ann. appl. Biol. 61: 175.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.