Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Potato X potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by C. Büchen-Osmond, 1987.

Nomenclature

Synonyms

potato latent virus, potato mild mosaic virus.

Acronym

PVX

Strains

Indian turnip strain; Samad et al., 1991.

ICTV decimal code

56.0.1.0.018

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Solanum tuberosum - symptoms vary, some strains symptomless, others induce necrotic streaks.
• Brassica campestris ssp. rapa - mild mosaic mottling and distortion of leaves, plant stunting.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Probably distributed worldwide (in potato growing areas). Spreads in India.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Datura stramonium - systemic chlorotic rings, then mosaic and mottling.
• Nicotiana tabacum - systemic ringspot or mottle.
  Some strains symptomless at high glasshouse temperatures.

Maintenance and propagation hosts

Nicotiana tabacum.

Assay hosts (Local lesions or Whole plants)

Gomphrena globosa (L).

Susceptible host species

• Brassica campestris ssp. rapa
• Datura stramonium
• Gomphrena globosa
• Nicotiana tabacum
• Solanum tuberosum

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Cruciferae (1/1)
• Solanaceae (3/3)

Physical and biochemical properties

Properties of particles in sap

TIP: 68-76 °C. LIV: 40-60 days. DEP: log₁₀ minus 5-6. Leaf sap contains many virions.

Purification method

Francki and McLean (1968).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 515 nm; 13 nm wide (Brandes, 1964). Axial canal obscure; 3.4 nm in diameter. Basic helix obvious; pitch of basic helix 3.4 nm (Varma et al., 1968).

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 117.7 S. Isoelectric point pH 4.4.

Biochemical properties

Virions contain 6 % nucleic acid (Knight, 1963); 94 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 6.435 kb. Genome unipartite; largest (or only) genome part 6.435 kb. Base composition 22 % G; 32 % A; 24 % C; 22 % U. 5´ terminus of RNA has a methylated nucleotide cap. Infectivity retained when deproteinised with proteases; retained when deproteinised with phenol or detergent.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00344 Em(40)_vi:PVXX3 Gb(84)_vi:PVXX3 Potato virus X genomic RNA. 4/92 6,435bp.
• M31541 Em(40)_vi:PVXKPA Gb(84)_vi:PVXKPA Potato virus X 166K RNA, complete cds; 24K RNA, complete cds; 12K RNA, complete cds; 8K RNA,
• M38480 Em(40)_vi:PVXGEN Gb(84)_vi:PVXGEN Potato virus X complete genome. 3/92 6,436bp.
• M38655 Em(40)_vi:PVXCT23 Gb(84)_vi:PVXCT23 Potato X virus coat protein gene, complete cds. 10/90 861bp.
• M63141 Em(40)_vi:PVXCGA1 Gb(84)_vi:PVXCGA Potato virus X complete genome. 2/92 6,432bp.
• M72416 Em(40)_vi:PVXCG Gb(84)_vi:PVXCG Potato virus X complete genome. 10/93 7,568bp.
• M95516 Em(40)_vi:PVXCOPR Gb(84)_vi:PVXCOPR Potato virus X mRNA, complete cds. 6/92 6,435bp.
• X05198 Em(40)_vi:POPVX3 Gb(84)_vi:POPVX3 Potato virus X genome. 10/93 6,435bp.
• X12804 Em(40)_vi:PVXCOAT Gb(84)_vi:PVXCOAT Potato Virus X (PVX) mRNA for viral coat protein. 9/93 1,106bp.
• X55802 Em(40)_vi:POPOVX Gb(84)_vi:POPOVX Potato Virus X complete genomic RNA. 4/92 6,432bp.
• X62442 Em(40)_vi:PVXANPII Gb(84)_vi:PVXANPII Potato virus X artificial neomycin phosphotransferase II mRNA 5´-flank (SEQ1). 9/92 115bp.
• X62443 Em(40)_vi:PVXANPTII Gb(84)_vi:PVXANPTII Potato virus X artificial neomycin phosphotransferase II mRNA 5´-flank (SEQ2). 9/92 88bp.
• X62444 Em(40)_vi:PVXANPT Gb(84)_vi:PVXANPT Potato virus X artificial neomycin phosphotransferase II mRNA 5´-flank (SEQ3). 9/92 62bp.
• X62445 Em(40)_vi:PVXANPTR Gb(84)_vi:PVXANPTR Potato virus X artificial neomycin phosphotransferase II mRNA 5´-flank (SEQ4). 9/92 92bp.
• X65015 Em(40)_vi:PVXCP Gb(84)_vi:PVXCP Potato Virus X genomic RNA for coat protein. 6/92 789bp
• X72214 Em(40)_vi:PVXHBRNA Gb(84)_vi:PVXHBRNA Potato Virus X strain HB RNA. 11/93 6,432bp.
• X88781 Em(44)n:Pvxcpdy Gb(90)_vi:Pvxcpdy Potato virus X coat protein gene, DY strain. 6/95 744bp.
• X88782 Em(44)n:Pvxcpex Gb(90)_vi:Pvxcpex Potato virus X coat protein gene, EX strain. 6/95 744bp.
• X88783 Em(44)n:Pvxcpkp Gb(90)_vi:Pvxcpkp Potato virus X coat protein gene, KP strain. 6/95 711bp.
• X88784 Em(44)n:Pvxcpnl1 Gb(90)_vi:Pvxcpnl1 Potato virus X coat protein gene, Nl1 strain. 6/95 711bp.
• X88785 Em(44)n:Pvxcpnl4 Gb(90)_vi:Pvxcpnl4 Potato virus X coat protein gene, Nl4 strain. 6/95 744bp.
• X88786 Em(44)n:Pvxcpws2 Gb(90)_vi:Pvxcpws2 Potato virus X coat protein gene, WS2 strain. 6/95 708bp.
• X88787 Em(44)n:Pvxcpxa Gb(90)_vi:Pvxcpxa Potato virus X coat protein gene, XA strain. 6/95 711bp.
• X88788 Em(44)n:Pvxcpxs Gb(90)_vi:Pvxcpxs Potato virus X coat protein gene, XS strain. 6/95 711bp.
• Z23256 Em(40)_vi:PVCOAPRA Gb(84)_vi:PVCOAPRA Potato virus X coat protein RNA and 3 ORF's, complete CDS's. 12/93 6,433bp.
• Z29333 Em(40)_vi:PVX8KCP1 Gb(84)_vi:PVX8KCP1 Potato virus X (P551) mRNA for 8K protein and coat protein. 1/94 877bp.
• Z29334 Em(40)_vi:PVX8KCP2 Gb(84)_vi:PVX8KCP2 Potato virus X (CP2) mRNA for 8K protein and coat protein. 1/94 874bp.
• Z29335 Em(40)_vi:PVX8KCP3 Gb(84)_vi:PVX8KCP3 Potato virus X (S6111) mRNA for 8K protein and coat protein. 1/94 873bp.
• Z34261 Gb(84)_vi:PVXCPG Potato virus X (MS) coat protein gene. 6/94 711bp.
• M27662 Em(43)_vi:Pvxorf Gb(89)_vi:Pvxorf Potatovirus X (clone pX 1) gene fragment, encoding a partial ORF. 1/95 502bp.
• U19790 Gb(89)n:Pvu19790 Potato virus X coat protein mRNA, complete cds. 6/95 789bp.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 30000; coat protein. Amino acid composition: Shaw and Larson (1962); Shaw et al. (1962); Miki and Knight (1968).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in mesophyll and less frequently in the epidermis; in cytoplasm. Inclusions mainly near the nucleus present in infected cells; are amorphous X-bodies (Kozar and Sheludko, 1969); they contain virions.

Taxonomy and relationships

Potexvirus

Virus(es) with serologically related virions

White clover mosaic, hydrangea ringspot, cactus X and clover yellow mosaic viruses, but distantly.

Comments and References

References

• Bercks, R, (1970). CMI/AAB Descr. Pl. Viruses No. 4, 4 pp.
• Brandes, J. (1964). Mitt. biol. BundesAnst. Land. Forstw. 110: 130.
• Dolya, V.V., Grama, D.P., Morozov, S.Y. and Atabekov, J.G. (1987). FEBS Lett. 214: 308.
• Francki, R.I.B. and McLean, G. (1968). Aust. J. biol. Sci. 21: 1311.
• Hussman, M.J., Linthorst, H.J.M., Bol. J.F. and Cornellisen, B.J.C. (1988). J. gen. Virol. 69: 1789.
• Knight, C.A. (1963). Protoplasmatologia 4: 177.
• Koenig, R. and Lesemann, D.-E. (1989). CMI/AAB Descr. Pl. Viruses No. 354, 5 pp.
• Kozar, I.E. and Sheludko, Y.M. (1969). Virology 38: 220.
• Miki, T. and Knight, C.A. (1968). Virology 36: 168.
• Morozov, S.Y., Miroshnichenko, N.A., Solovyev, A.G., Fedorkin, O.N., Zelenina, D.A., Lukasheva, L.I., Karasev, A.V., Dolya, V.V. and Atabekov, J.G. (1991). J. gen. Virol. 72: 2039.
• Paul, H.L. (1959). Arch. Microbiol. 32: 416.
• Samad, A., Naqvi, Q.A., Garg, I.D. and Thouvenel, J.-C. (1991). J. Phytopath. 133: 65.
• Shaw, J.G. and Larson, R.H. (1962). Phytopathology 52: 170.
• Shaw, J.G., Reichmann, M.E. and Hatt, D.L. (1962). Virology 18: 79.
• Skryabin, K.G., Morozov, S.Y., Kraev, A.S., Rozanov, M.V., Chernov, B.K., Lukasheva, L.I. and Atabekov, J.G. (1988). FEBS Lett. 240: 33.
• Smith, K.M. (1931). Proc. R. Soc. 109: 251.
• Varma, A., Gibbs, A.J., Woods, R.D. and Finch, J.T. (1968). J. gen. Virol. 2: 107.
• Tomashevskaya, O.L., Solvyev, A.G., Karpova, O.V., Fedorkin, O.N., Rodionova, N.P., Morozov, S.Y. and Atabekov, J.G. (1993). J. gen. Virol. 74: 2717.
• Wilson, H.R. and Tollin, P. (1969). J. gen. Virol. 5: 151.
• Wodnar-Filipowicz, A., Skizeczkowski, L.J. and Filipowicz, W. (1990). FEBS Lett. 109: 151.

Illustrations

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.