Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Potato black ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by L.F. Salazar, 1987.

Nomenclature

Synonyms

potato calico strain of tobacco ringspot virus (Salazar and Harrison, 1978), Andean potato calico strain of tobacco ringspot virus (Fribourg, 1977).

Acronym

PBRSV

Strains

potato calico strain.

ICTV decimal code

18.0.3.0.024

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Solanum tuberosum - necrotic ringspots. Potato calico strain causes general leaf chlorosis in some cultivars, especially cv. Ticahuasi.

Transmission

Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in the South and Central American region; Peru.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Beta vulgaris - necrotic spots and rings; systemic chlorotic or necrotic rings.
• Lycopersicon esculentum cv. Kondine Red - systemic necrotic rings spots and/or line patterns.
• Cyamopsis tetragonoloba - general systemic necrosis.
• Chenopodium amaranticolor, C. quinoa - necrotic local lesions; systemic necrosis.
• Nicotiana tabacum - systemic necrotic ringspots and line patterns.
• Solanum tuberosum ssp. tuberosum - necrotic spots; systemic necrotic ringspots.
• Catharanthus roseus - chlorotic spots; systemic necrosis.

Maintenance and propagation hosts

Nicotiana clevelandii, N. tabacum cv. Samsun, N. benthamiana.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), Chenopodium quinoa (L) and Nicotiana clevelandii (S).

Susceptible host species

• Amaranthus caudatus
• Apium graveolens
• Beta vulgaris
• Catharanthus roseus
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium foetidum
• Chenopodium foliosum
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Cyamopsis tetragonoloba
• Datura stramonium
• Datura tatula
• Daucus carota
• Dianthus barbatus
• Lactuca sativa
• Lycopersicon esculentum
• Nicandra physalodes
• Nicotiana benthamiana
• Nicotiana bigelovii
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Physalis peruviana
• Pisum sativum
• Solanum tuberosum
• Solanum tuberosum ssp. andigena × S. tuberosum ssp. tuberosum
• Solanum tuberosum ssp. tuberosum
• Stellaria media
• Tetragonia tetragonioides
• Vigna unguiculata

Insusceptible host species

• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Apocynaceae (1/1)
• Caryophyllaceae (2/2)
• Chenopodiaceae (8/8)
• Compositae (1/1)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (4/4)
• Solanaceae (17/17)
• Tetragoniaceae (1 /1)
• Umbelliferae (2/2)

Families containing insusceptible hosts

• Gramineae (1/1)

Sources of host-range data

Salazar and Harrison (1978).

Physical and biochemical properties

Properties of particles in sap

TIP: 58-60 °C (in Nicotiana clevelandii sap). LIV: 9-10 days. DEP: log₁₀ minus 4. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions. Electron microscopy: it is better to mount virions in 1% UA.

Purification method

Salazar and Harrison (1978).

Particle morphology

Virions isometric; not enveloped; 25 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 117 S (B); of the other(s) 84 S (M), or 49 S (T). A₂₆₀/A₂₈₀ ratio 1.97 (B), or 1.78 (M), or 1.03 (T).

Biochemical properties

Virions contain 41 % nucleic acid (B), or 28 % nucleic acid (M), or 0 % nucleic acid (T); 59 % protein (B), or 72 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded. Total genome size 11.2 kb. Genome of two parts; largest (or only) genome part the larger 7 kb; the 2nd largest 4.2 kb. Genomic nucleic acid isolated by Salazar and Harrison (1978). Infectivity lost when deproteinised with proteases; retained when deproteinised with phenol or detergent.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 59000. Method of preparation: Salazar and Harrison (1978).

Cytopathology

Virions found in mesophyll; in cytoplasm. Inclusions absent from infected cells.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically related virions

Tobacco ringspot and eucharis mottle viruses.

Virus(es) with serologically unrelated virions

Arabis mosaic, artichoke Italian latent, cacao necrosis, cherry leaf roll, grapevine fanleaf, myrobalan latent ringspot, raspberry ringspot, strawberry latent ringspot, tomato black ring and tomato ringspot viruses. Although the virus is serologically distantly related to tobacco ringspot and eucharis mottle viruses, the viruses do not cross-protect in plants and do not form recombinants; potato black ringspot virus is, therefore, considered to be a distinct virus.

Best tests for diagnosis

Serology is the only way to distinguish this virus from others with which it is often naturally associated.

Comments and References

References

• Fribourg, C.E. (1977). Phytopathology 67: 174.
• Salazar, L.F. and Harrison, B.D. (1977). Nature, Lond 265: 337.
• Salazar, L.F. and Harrison, B.D. (1978b). Ann. appl. Biol. 90: 387.
• Salazar, L.F. and Harrison, B.D. (1978). Ann. appl. Biol. 90: 375.
• Salazar, L.F. and Harrison, B.D. (1979). CMI/AAB Descr. Pl. Viruses No. 206, 4 pp.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.