Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Plantain X potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J. Hammond, 1987.

Nomenclature

Acronym

PlVX

ICTV decimal code

56.0.1.0.016

Host range and symptoms

Natural host range and symptoms

• Plantago lanceolata - no obvious symptoms.

Transmission

Virus transmitted by mechanical inoculation.

Ecology and control

Studies reported by Hammond (1982).

Geographical distribution

Spreads in the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Nicotiana clevelandii - systemic necrotic flecking when grown in bright light.
• N. × edwardsonii - necrotic flecking of inoculated and systemically infected leaves (white ringspots at 32ºC).
• Plantago rugelii - spreading local and systemic necrotic spots.

Diagnostically insusceptible host species

Tetragonia tetragonioides, Montia perfoliata, Brassica campestris ssp. rapa, Cucumis sativus, Gomphrena globosa.

Maintenance and propagation hosts

Nicotiana clevelandii, N. × edwardsonii.

Assay hosts (Local lesions or Whole plants)

Nicotiana × edwardsonii (W) and Plantago rugelii (L).

Susceptible host species

• Amaranthus caudatus
• Chenopodium amaranticolor
• Datura metel
• Datura stramonium
• Hyoscyamus niger
• Lycopersicon esculentum
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana rustica
• Nicotiana tabacum
• Nicotiana × edwardsonii
• Petunia × hybrida
• Phaseolus vulgaris
• Plantago lanceolata
• Plantago rugelii
• Vigna unguiculata
• Zinnia elegans

Insusceptible host species

• Brassica campestris ssp. chinensis
• Brassica campestris ssp. pekinensis
• Brassica campestris ssp. rapa
• Chenopodium hybridum
• Chenopodium quinoa
• Cucumis sativus
• Gomphrena globosa
• Momordica balsamina
• Montia perfoliata
• Nicotiana glutinosa
• Pisum sativum
• Tetragonia tetragonioides
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/2)
• Chenopodiaceae (1/3)
• Compositae (1/1)
• Leguminosae-Papilionoideae (2/4)
• Plantaginaceae (2/2)
• Solanaceae (10/11)

Families containing insusceptible hosts

• Amaranthaceae (1/2)
• Chenopodiaceae (2/3)
• Cruciferae (3/3)
• Cucurbitaceae (2/2)
• Leguminosae-Papilionoideae (2/4)
• Portulacaceae (1/1)
• Solanaceae (1/11)
• Tetragoniaceae (1/1)

Sources of host-range data

Hammond and Hull (1981).

Physical and biochemical properties

Properties of particles in sap

TIP: 60-70 °C. Leaf sap contains many virions. Electron microscopy: fixation not necessary. Best detail with UA and UF.

Purification method

Hammond and Hull (1981).

Particle morphology

Virions filamentous; sometimes have a loose external helical structure in negatively stained epidermal dips (Hammond and Hull, 1981); not enveloped; usually flexuous; with a clear modal length; of 570-580 nm; 12 nm wide. Axial canal obscure. Basic helix obvious (in UA or UF); pitch of basic helix 3.4 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 119 S. Density 1.31 g cm^-3 in CsCl. Density 1.259 in Cs₂SO₄. A₂₆₀/A₂₈₀ ratio 1.19 (partially corrected).

Biochemical properties

Virions 94-95 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 6.5 kb. Genome unipartite; largest (or only) genome part 6.5 kb. Genomic nucleic acid isolated by Wilcockson and Hull; the SDS - sodium perchlorate method; or the phenol-chloroform method is probably equally as efficient.

Features of proteins

Virion protein(s) one; M_r 26000; coat protein. Method of preparation: Francki and McLean (1968). Amino acid composition: Hammond and Hull (1981).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are unusual in shape; whorl-like arrays; they contain virions.

Taxonomy and relationships

Potexvirus

Virus(es) with serologically unrelated virions

Cactus X, clover yellow mosaic, cymbidium mosaic, narcissus mosaic, papaya mosaic (plantago severe mottle strain), potato X and white clover mosaic viruses.

Additional comments on relationships

No significant sequence homologies were found between plantago X, potato X and papaya mosaic viruses by cDNA/RNA hybridization.

Best tests for diagnosis

Plantain X virus does not infect Gomphrena globosa, whereas the plantago severe mottle strain of papaya mosaic virus does. The latter also does not infect Plantago lanceolata and Nicotiana spp.

Comments and References

References

• Gracia, O., Koenig, R. and Lesemann, D.-E. (1983). Phytopathology 73: 1488.
• Hammond, J. (1977). Rep. John Innes Inst. 1976, p.109.
• Hammond, J. (1983). Adv. Virus Res. 27: 103.
• Hammond, J. and Hull, R. (1981). J. gen. Virol. 54: 75.
• Hammond, J. and Hull, R. (1983). CMI/AAB Descr. Pl. Viruses No. 266, 4 pp.
• Rowhani, A. and Petersen, J.F. (1980). Can. J. Pl. Path. 2: 12.
• Wilcockson, J. and Hull, R. (1974). J. gen. Virol. 23: 107.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.