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Descriptions and Lists from the VIDE Database

Pepper ringspot tobravirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D.J. Robinson, 1987.

Nomenclature

Synonyms

artichoke yellow band virus (Chagas, Flores and Caner, 1970; Harrison and Robinson, 1986), tobacco rattle virus - CAM, tobacco rattle virus - serotype III.

Acronym

PepRSV

ICTV decimal code

72.0.1.0.003

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Capsicum annuum - ringspots.
• Cynara scolymus - yellow banding.
• Lycopersicon esculentum - ringspots and yellow banding.
• Bidens pilosa.

Transmission

Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants.

Ecology and control

Studies reported by reviewed by Harrison (1977).

Geographical distribution

Spreads in the South and Central American region; Brazil.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - necrotic local lesions, not systemic.
• Cucumis sativus - chlorotic or necrotic local lesions, not systemic.
• Nicotiana tabacum cv. Samsun NN - necrotic rings and lines, systemic.
• N. tabacum cv. White Burley - symptomless, systemic.
• Phaseolus vulgaris - pinpoint necrotic local lesions, not systemic.
• Pisum sativum and Vicia faba - small necrotic local lesions, not systemic.

Maintenance and propagation hosts

Nicotiana clevelandii, N. tabacum cv. White Burley.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), Phaseolus vulgaris (L).

Susceptible host species

• Beta vulgaris
• Bidens pilosa
• Capsicum annuum
• Catharanthus roseus
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Cynara scolymus
• Datura stramonium
• Gomphrena globosa
• Lycopersicon esculentum
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana tabacum
• Ocimum basilicum
• Petunia × hybrida
• Phaseolus vulgaris
• Pisum sativum
• Spinacia oleracea
• Tetragonia tetragonioides
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Apocynaceae (1/1)
• Chenopodiaceae (6/6)
• Compositae (2/2)
• Cucurbitaceae (1/1)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (3/3)
• Solanaceae (11/11)
• Tetragoniaceae (1 /1)

Physical and biochemical properties

Properties of particles in sap

TIP: 80-85 °C. LIV: more than 42 days. DEP: log₁₀ minus 5-6. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains few virions. Electron microscopy: preservation in uranyl formate/NaOH. Fine detail in UA.

Purification method

Harrison and Nixon (1959).

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with a clear modal length; of 52 and 197 nm; 22 nm wide. Axial canal obvious; 4 nm in diameter. Basic helix obvious; pitch of basic helix 2.5 nm.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest (long) virions 305 S; of the other(s) 163 S (short virions). Density 1.306 g cm^-3 in CsCl (unfixed). Density 1.27 g cm^-3 in sucrose. A₂₆₀/A₂₈₀ ratio 1.15.

Biochemical properties

Virions contain 5 % nucleic acid; 95 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 9.6 kb. Genome of two parts; largest (or only) genome part the larger 7.5 kb; the 2nd largest 2.1 kb. Genomic nucleic acid isolated by Harrison and Nixon (1959); Darby and Minson, (1972); Cooper and Mayo, (1972). Base composition 25.5 % G (RNA-1), or 24.3 % G (RNA-2); 29.5 % A (RNA-1), or 26.1 % A (RNA-2); 16.5 % C (RNA-1), or 17.6 % C (RNA-2); 28.5 % U (RNA-1), or 32 % U (RNA-2). 5´ terminus of RNA has a methylated nucleotide cap (reported only on RNA-1, but probably on both). Infectivity retained when deproteinised with proteases; retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity. Genome has no tRNA-like activity. Nucleotide sequence references: Bergh et al. (1985).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• L23972 Em(40)_vi:PPNRNA Gb(84)_vi:PPNRNA Pepper ringspot viral 12k, 29k 136k and 195k protein RNA, complete cds. 11/93 6,828bp. 1 sequence.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA. Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 22000; coat protein. Method of preparation: Semancik (1970); Ghabrial and Lister (1973).

Virus-coded non-virion proteins identified by genomic sequence analysis (Bergh et al. (1985)); three proteins found. M_r of the largest 170000. M_r of 2nd largest 140000. M_r of 3rd 30000.

Replication

Genome replicates in cytoplasm. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant, except xylem; in cytoplasm (mostly near mitochondria, but not in them). Inclusions present in infected cells (by NM forms); are amorphous X-bodies.

Taxonomy and relationships

Tobravirus

Virus(es) with serologically related virions

Tobacco rattle and pea early-browning viruses, but distantly.

Additional comments on relationships

Although there is distant sequence homology with other tobraviruses, these are not usually detected in hybridization tests.

Comments and References

General comments

For many years pepper ringspot virus was thought to be a serotype of tobacco rattle virus, and some research work did not distinguish between them.

References

• Bergh, S.T., Koziel, M.G., Huang, S.C., Thomas, R.A., Gilley, D.P. and Siegel, A. (1985). Nucl. Acids Res. 13: 8507.
• Chagas, C.M., Flores, E. and Caner, J. (1970). Biologico 35: 271.
• Cooper, J.I. and Mayo, M.A. (1972). J. gen. Virol. 16: 285.
• Darby, G. and Minson, A.C. (1972). J. gen. Virol. 14: 199.
• Ghabrial, S.A. and Lister, R.M. (1973). Virology 52: 1.
• Harrison, B.D. (1970). CMI/AAB Descr. Pl. Viruses No. 12, 4 pp.
• Harrison, B.D. (1977). Ann. Rev. Phytopath. 15: 331.
• Harrison, B.D. and Nixon, H.L. (1959). J. gen. Microbiol. 21: 591.
• Harrison, B.D. and Robinson, D.J. (1986). In: The Plant Viruses Vol 2, The Rod-Shaped Viruses, p. 339; eds M.H.V. van Regenmortel and H. Fraenkel-Conrat. Plenum Press, New York.
• Robinson, D.J. and Harrison, B.D. (1989). CMI/AAB Descr. Pl. Viruses No. 347, 4 pp.
• Semancik, J.S. (1970). Virology 40: 618.
• Silberschmidt, K. (1962-63). Phytopath. Z. 46: 209.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.