Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Pepper Moroccan tombusvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D. Gallitelli, 1991.

Nomenclature

Synonyms

Moroccan pepper virus.

Acronym

MPV

ICTV decimal code

74.0.1.0.008

Host range and symptoms

Natural host range and symptoms

Symptoms persist and vary cyclically over a few weeks. Symptoms stunting, leaf malformations, lethal systemic necrosis.
• Capsicum annuum - stunting, deformation, leaf and flower abscission, lethal systemic necrosis.
• Lycopersicon esculentum - leaf mottling and malformation, stunting.
• Pelargonium zonale - leaf malformation, stunting.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by grafting.

Geographical distribution

Spreads in Morocco. Found, but with no evidence of spread, in Germany.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show necrotic local lesions, systemic necrosis, stunting.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. murale, Cucumis sativus, Cucurbita pepo, Nicotiana tabacum, N. glutinosa, Petunia × hybrida, Phaseolus vulgaris, Vicia faba - necrotic local lesions.
• Capsicum annuum, Nicotiana benthamiana, N. clevelandii, Spinacia oleracea - necrotic local lesions; systemic necrosis.

Maintenance and propagation hosts

Nicotiana benthamiana, N. clevelandii.

Assay hosts (Local lesions or Whole plants)

Capsicum annuum (W), Datura stramonium (L), Nicotiana benthamiana (W), Nicotiana glutinosa (L), Spinacia oleracea (W).

Susceptible host species

• Antirrhinum majus
• Capsicum annuum
• Chenopodium amaranticolor
• Chenopodium foetidum
• Chenopodium murale
• Chenopodium quinoa
• Cucumis melo
• Cucumis sativus
• Cucurbita pepo
• Datura metel
• Datura stramonium
• Gomphrena globosa
• Lupinus albus
• Lycopersicon esculentum
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana tabacum
• Ocimum basilicum
• Pelargonium zonale
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Pisum sativum
• Solanum melongena
• Spinacia oleracea
• Vicia faba
• Vigna unguiculata ssp. sesquipedalis
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (5/5)
• Compositae (1/1)
• Cucurbitaceae (3/3)
• Geraniaceae (1/1)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (5/5)
• Scrophulariaceae (1/1)
• Solanaceae (13 /13)

Sources of host-range data

Fischer and Lockhart (1977); Vetten and Koenig (1983).

Physical and biochemical properties

Properties of particles in sap

TIP: 80-85 °C. LIV: 49-56 days. DEP: log₁₀ minus 6-7. Leaf sap contains many virions.

Purification method

Gallitelli and Russo (1987).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; neither clearly angular or nor rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 140 S. Density 1.36 g cm^-3 in CsCl.

Biochemical properties

Virions contain 18 % nucleic acid; 82 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 4.7 kb. Genome unipartite; largest (or only) genome part 4.7 kb. Genomic nucleic acid isolated by Gallitelli and Russo (1987). Infectivity retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 43000; coat protein. Method of preparation: Laemmli (1970).

Cytopathology

Virions found in leaves, roots and mesophyll; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are membranous bodies; they do not contain virions. Other cellular changes: see Martelli et al. (1988).

Taxonomy and relationships

Tombusvirus: Tombusviridae

Virus(es) with serologically related virions

Artichoke mottled crinkle, eggplant mottled crinkle, grapevine Algerian latent, pelargonium leaf curl, petunia asteroid mosaic and tomato bushy stunt viruses.

Virus(es) with serologically unrelated virions

Carnation Italian ringspot, cymbidium ringspot and Neckar River tombusviruses.

Additional comments on relationships

Dot blot hybridization tests indicate that the virus has 10% nucleotide homology with tomato bushy stunt tombusvirus.

Comments and References

References

• Fischer, H.V. and Lockhart, B.E. (1977). Phytopathology 67: 1352.
• Gallitelli, D. and Russo, M. (1987). J. Phytopath. 119: 106.
• Laemmli, U.K. (1970). Nature, Lond. 227: 680.
• Martelli, G.P., Gallitelli, D. and Russo, M. (1988). In: The Plant Viruses, Vol. 3, Polyhedral Virions with Monopartite RNA Genomes, p. 13; ed. R. Koenig. Plenum Press, New York.
• Martelli, G.P., Russo, M. and Gallitelli, D. (1988). AAB Descr. Pl. Viruses No. 352. 8pp.
• Vetten, H.J. and Koenig, R. (1985). Phytopath. Z. 108: 215.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.