Plant Viruses
Online 
Descriptions and Lists from
the VIDE Database
Pelargonium
zonate spot ourmiavirus
Index
Data collated by D. Gallitelli, 1987.
Nomenclature
Acronym
ICTV decimal code
Host range and symptoms
First reported
in Lycopersicon esculentum; from Apulia, Southern Italy; by Martelli and
Cirulli (1969).
Natural host range and symptoms
Symptoms disappear soon
after infection.
- Lycopersicon esculentum - concentric yellow bands, then
necrotic stunting.
- Cynara scolymus - foliar malformations.
- Chrysanthemum segetum - yellow mottling.
- Diplotaxis
erucoides - symptomless.
- Pelargonium zonale - concentric
yellow bands.
Transmission
Virus transmitted by mechanical inoculation;
transmitted by grafting; transmitted by seed; transmitted by pollen to the seed.
Ecology and control
Studies reported by Gallitelli (1982);
Quacquarelli et al. (1969); Vovlas et al. (1986).
Geographical distribution
Spreads in Italy (Apulia).
Experimental host range
Several (3-9) families
susceptible.
Diagnostically susceptible host species and
symptoms
- Cucumis sativus - chlorotic local lesions.
- Nicotiana glutinosa - chlorotic or necrotic local lesions,
systemic mosaic and marginal necrosis.
- Nicotiana tabacum cv. Xanthi
- chlorotic rings, systemic mottling.
- Cucurbita pepo -
chlorotic or necrotic local lesions, death.
- Phaseolus vulgaris -
chlorotic or necrotic local lesions.
- Lycopersicon esculentum -
concentric yellow bands, then necrosis.
Diagnostically insusceptible
host species
Callistephus chinensis, Datura stramonium, Nicotiana
benthamiana, Ocimum basilicum, Pisum sativum.
Maintenance and
propagation hosts
Cucumis sativus, Lycopersicon esculentum,
Nicotiana glutinosa, N. tabacum cv. Xanthi.
Assay hosts (Local lesions or Whole plants)
Chenopodium quinoa (L), C. amaranticolor (L), Cucumis
sativus (W), Nicotiana glutinosa (W), Vigna radiata (L).
Susceptible host species
Insusceptible host species
Families containing susceptible hosts
Families containing
insusceptible hosts
Sources of host-range data
Quacquarelli et al. (1979); Gallitelli (1982); Vovlas et al.
(1986).
Physical and
biochemical properties
Properties of particles in sap
TIP: 40
°C. LIV: 0.3 days. DEP: log10 minus 2. Leaf sap contains many virions.
Electron microscopy: fix with formaldehyde before mounting in KPT.
Purification method
Gallitelli;
homogenise Nicotiana glutinosa leaves in 20mM sodium phosphate containing
20 mM sodium thioglycollate and 20 mM DIECA, clarify by adjusting pH to 4.8, and
precipitate virions with PEG.
Particle morphology
Virions bacilliform; not enveloped;
35 nm in length; 25 nm wide.
Physical properties
Three sedimenting components in
purified preparations; sedimentation coefficient of the fastest 118 S
(BV, which is probably an aggregate of TV); of the other(s) 80 S (TV), or
90 S (MV). Density 1.286 g cm-3 in CsCl (Cs2SO4), or 1.346 g
cm-3 in CsCl (CsCl; formaldehyde-stabilised virions).
Biochemical properties
Virions contain 18 % nucleic acid
(TV), or 18 % nucleic acid (MV), or 18 % nucleic acid (BV).
Genome consists of RNA; single-stranded. Total genome size 7.7 kb. Genome
of two parts; largest (or only) genome part the larger 4.4 kb; the 2nd largest
3.3 kb. Genomic nucleic acid isolated by Gallitelli; use phenol-SDS. Infectivity
retained when deproteinised with phenol or detergent. Additional factor not
required for infectivity.
Features of the genome
Non-genomic nucleic acid
found in the virions; is of unknown origin and function; perhaps a
satellite-like RNA is associated with some strains.
Features of proteins
Virion protein(s) one;
Mr 23000. Method of preparation: Gallitelli (1982).
Replication
Replication does not depend on a helper
virus.
Cytopathology
Virions found in leaves, mesophyll,
epidermis and phloem; in cytoplasm, in nuclei, and in nucleoli. Other cellular
changes: damage of chloroplasts and vesicles containing fibrillar material.
Taxonomy and
relationships
Virus(es) with serologically unrelated virions
Alfalfa mosaic, apple mosaic, arabis mosaic, artichoke Italian
latent, artichoke mottled crinkle, artichoke vein banding, broad bean wilt,
brome mosaic, carnation ringspot, cherry leaf roll, cherry rasp leaf, chicory
yellow mottle, cacao necrosis, cucumber mosaic, grapevine Bulgarian latent,
grapevine chrome mosaic, grapevine fanleaf, myrobalan latent ringspot,
pelargonium leaf curl, prune dwarf, prunus necrotic ringspot, radish mosaic,
raspberry bushy dwarf, raspberry ringspot, southern bean mosaic, sowbane mosaic,
tobacco necrosis, tobacco ringspot, tobacco streak, tomato black ring, tomato
ringspot, Tulare apple mosaic and turnip yellow mosaic viruses.
Additional comments on relationships
The virus shares properties with epirus cherry, cassava Ivorian
bacilliform, olive latent 2 and melon Ourmia viruses.
Best tests for
diagnosis
Comments and
References
References
- Castellano, M.A. and
Martelli, G.P. (1981). Phytopathol. Medit. 20: 64.
- Gallitelli,
D. (1982). Ann. appl. biol. 100: 457.
- Gallitelli, D.,
Martelli, G.P. and Quacquarelli, A. (1983). CMI/AAB Descr. Pl. Viruses
No. 272, 4 pp.
- Martelli, G.P. and Cirulli, M. (1969). Phytopathol.
Medit. 8: 154.
- Quacquarelli, A. and Gallitelli, D. (1979).
Phytopathol. Medit. 18: 61.
- Vovlas, C., Gallitelli, D. and
Difranco, A. (1986). Inform. Fitopatol. 36: 9.
Cite this publication as:
Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.)
(1996 onwards).
`Plant Viruses Online: Descriptions and Lists from the VIDE Database.
Version: 20th August 1996.' URL
http://biology.anu.edu.au/Groups/MES/vide/
Dallwitz (1980)
and
Dallwitz, Paine and Zurcher (1993)
should also be cited.
Please send comments, corrections and suggestions to:
vide-manager@biology.anu.edu.au