Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Peanut yellow spot tospovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D.V.R. Reddy, 1989.

Nomenclature

ICTV decimal code

11.0.5.DE.01

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Arachis hypogaea - small chlorotic local lesions, which coalesce then become necrotic.

Transmission

Transmitted by a vector; an insect; Scirtothrips dorsalis; Thysanoptera. Transmitted in a semi-persistent manner. Virus retained when the vector moults; not transmitted congenitally to the progeny of the vector; transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in India and Thailand.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Vigna unguiculata - large chlorotic local lesions becoming necrotic.
• Petunia × hybrida - necrotic local lesions.
• Chenopodium quinoa - chlorotic local lesions.
• Datura stramonium - chlorotic local lesions with necrotic rings.

Diagnostically insusceptible host species

Lycopersicon esculentum, Nicandra physalodes.

Maintenance and propagation hosts

Arachis hypogaea, Vigna mungo.

Assay hosts (Local lesions or Whole plants)

Vigna unguiculata (L), Petunia × hybrida (L), Chenopodium quinoa (L), Datura stramonium (L).

Susceptible host species

• Arachis hypogaea
• Chenopodium amaranticolor
• Chenopodium quinoa
• Datura stramonium
• Glycine max
• Nicotiana benthamiana
• Petunia × hybrida
• Phaseolus vulgaris
• Pisum sativum
• Vigna mungo
• Vigna radiata
• Vigna unguiculata

Insusceptible host species

• Lycopersicon esculentum
• Nicandra physalodes
• Nicotiana rustica

Families containing susceptible hosts

• Chenopodiaceae (2/2)
• Leguminosae-Papilionoideae (7/7)
• Solanaceae (3/6)

Families containing insusceptible hosts

• Solanaceae (3/6)

Physical and biochemical properties

Properties of particles in sap

TIP: 40-45 °C. LIV: less than 1 days (c. 4 hours at 25-30ºC). DEP: log₁₀ minus 2. Infectivity of sap decreased by treatment with di-ethyl ether. Leaf sap contains few virions. Electron microscopy: fix in 1.5% glutaraldehyde.

Purification method

Reddy: from Arachis hypogaea tissue; grind in 20mM phosphate buffer, pH 7.5, clarify. Add NaCl (0.2 M), PEG (2.1); centrifuge 10000 G for 15 minutes; resuspend in 10mM phosphate (pH 7.5) and centrifuge in sucrose gradients.

Particle morphology

Virions unusually shaped; quasi-isometric; enveloped; 70-90 nm in diameter; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 17.6 kb. Genome of three parts; largest (or only) genome part the largest 8.8 kb; the 2nd largest 5.4 kb; the 3rd largest 3.5 kb. Genomic nucleic acid isolated by Reddy; extract twice in phenol-cresol chloroform mixture. Infectivity lost when deproteinised with proteases; lost when deproteinised with phenol or detergent. Additional factor not required for infectivity.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) four; M_r of the largest 72000. M_r of 2nd largest 64000. M_r of 3rd largest 62000. M_r of 4th largest 31000. Virion proteins glycosylated. Virion proteins phosphorylated.

Replication

Genome replicates in cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm; associated with endoplasmic reticulum. Inclusions absent from infected cells.

Taxonomy and relationships

Tospovirus: Bunyaviridae

Virus(es) with serologically unrelated virions

Tomato spotted wilt virus.

Comments and References

References

• Anon. (1980). Rep. ICRISAT for 1979.
• Anon. (1985). Rep. ICRISAT 1984.
• Anon. (1986). Rep. ICRISAT 1985, p. 226.
• Anon. (1987). Rep. ICRISAT 1986, p. 227.
• Anon. (1988). Rep. ICRISAT 1987, p. 235.
• Reddy, D.V.R., Sudarshana, M.R., Ratna, A.S., Reddy, A.S., Kiran-Kumar, I. and Murthy, A.K. (1991). Proc. Tomato Spotted Wilt Virus Wksh. 17-19 April 1991, Beltsville, Maryland, U.S.A., p. 77.
• Wongkaew, S. (1983). Groundnut Improvement Project, Khon Kaen University, Progress Report 1983.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.