Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Peanut stunt cucumovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by G.I. Mink, 1980. Revised by P.L. Guy, 1990.

Nomenclature

Synonyms

robinia mosaic virus (Richter et al., 1979; Schmelzer, 1971; Zheng and Kung, 1984), black locust true mosaic virus, clover blotch virus (Richter et al., 1979).

Acronym

PSV

Strains

peanut common mosaic virus.

ICTV decimal code

10.0.4.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Robinia pseudoacacia - mosaic and leaf malformation.
• Trifolium repens, T. pratense, T. vesiculosum, T. incarnatum, T. subterraneum, Medicago sativa - chlorotic mottling.
• Phaseolus spp. - chlorotic local lesions and mottling, then mosaic, elongated misshapen trifoliate leaves in cv. Bountiful.
• Arachis hypogaea - stunting, mild mottle, pod malformation.
• Vicia sp. - systemic chlorotic mottle.
• Glycine max, Pisum sativum, Vigna angularis, Coronilla varia, Tephrosia sp., Nicotiana tabacum, Apium graveolens, Lupinus luteus - leaf chlorosis.

Transmission

Transmitted by a vector; an insect; Aphis craccivora, A. spiraecola and Myzus persicae, but not Aphis gossypii; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; transmitted by seed (0.1% in Arachis hypogaea).

Geographical distribution

Spreads in France, Japan, Korea D.P.R. (North), Korea Republic, Morocco, Poland, Spain, and the USA.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; systemic spotting.
• Spinacia oleracea, Tetragonia tetragonioides - systemic mottling.
• Glycine max - systemic chlorotic mottling.
• Phaseolus vulgaris - chlorotic or necrotic local lesions, systemic chlorotic mottling or mosaic.
• P. vulgaris cv. Bountiful - elongated mis-shapen trifoliate leaves.
• Pisum sativum - systemic chlorotic mottling, stunting.
• Vigna unguiculata - chlorotic local lesions; systemic vein clearing and severe epinasty.
• Datura stramonium - systemic chlorotic mottle with islands of green tissue.
• Nicotiana tabacum - light green and yellow rings, then systemic chlorotic areas.
• Tetragonia tetragonioides - systemic mottle.

Maintenance and propagation hosts

Phaseolus vulgaris, Pisum sativum, Vigna unguiculata, Nicotiana tabacum.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), Phaseolus vulgaris (L), Vigna unguiculata (L, W), Datura stramonium (W), Nicotiana tabacum (W).

Susceptible host species

• Amaranthus caudatus
• Apium graveolens
• Arachis hypogaea
• Beta vulgaris
• Brassica campestris ssp. rapa
• Capsicum annuum
• Capsicum frutescens
• Cassia occidentalis
• Catharanthus roseus
• Chenopodium amaranticolor
• Chenopodium foetidum
• Chenopodium murale
• Chenopodium quinoa
• Cicer arietinum
• Citrullus lanatus
• Coronilla varia
• Crotalaria spectabilis
• Cucumis melo
• Cucumis sativus
• Cucurbita maxima
• Cucurbita moschata
• Cucurbita pepo
• Cyamopsis tetragonoloba
• Datura metel
• Datura stramonium
• Dianthus barbatus
• Glycine max
• Gomphrena globosa
• Helianthus annuus
• Lens culinaris
• Lespedeza stipulacea
• Lobelia erinus
• Lupinus albus
• Lupinus angustifolius
• Lupinus luteus
• Lycopersicon esculentum
• Medicago sativa
• Melilotus albus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana tabacum
• Ocimum basilicum
• Petunia × hybrida
• Phaseolus acutifolius
• Phaseolus lunatus
• Phaseolus vulgaris
• Physalis floridana
• Physalis peruviana
• Pisum sativum
• Robinia pseudoacacia
• Sesamum indicum
• Sesbania exaltata
• Solanum melongena
• Spinacia oleracea
• Tephrosia vogelii
• Tetragonia tetragonioides
• Torenia fournieri
• Trifolium dubium
• Trifolium hybridum
• Trifolium incarnatum
• Trifolium pratense
• Trifolium repens
• Trifolium subterraneum
• Trifolium vesiculosum
• Vicia faba
• Vicia sativa
• Vicia villosa
• Vigna angularis
• Vigna radiata
• Vigna unguiculata
• Vigna unguiculata ssp. sesquipedalis
• Zinnia elegans

Insusceptible host species

• Antirrhinum majus
• Chenopodium capitatum
• Glycine max
• Helianthus annuus
• Momordica balsamina
• Solanum tuberosum

Families containing susceptible hosts

• Amaranthaceae (2/2)
• Apocynaceae (1/1)
• Campanulaceae (1/1)
• Caryophyllaceae (1/1)
• Chenopodiaceae (6/7)
• Compositae (2/2)
• Cruciferae (1/1)
• Cucurbitaceae (6/7)
• Labiatae (1/1)
• Leguminosae-Caesalpinioideae (1 /1)
• Leguminosae-Papilionoideae (34/34)
• Pedaliaceae (1/1)
• Scrophulariaceae (1/2)
• Solanaceae (13/14)
• Tetragoniaceae (1/1)
• Umbelliferae (1/1)

Families containing insusceptible hosts

• Chenopodiaceae (1/7)
• Compositae (1/2)
• Cucurbitaceae (1/7)
• Leguminosae-Papilionoideae (1/34)
• Scrophulariaceae (1/2)
• Solanaceae (1/14)

Sources of host-range data

Mink et al. (1969); Waterworth et al. (1973); Milbrath and Tolin (1977).

Physical and biochemical properties

Properties of particles in sap

TIP: 50-55 °C. LIV: 1 days (and several days for P strain). DEP: log₁₀ minus 3-4. Leaf sap contains many virions. Electron microscopy: aldehyde fixation necessary.

Purification method

Mink et al. (1969); Waterworth et al. (1973).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations (strain W), or four sedimenting components in purified preparations (strains B and P); sedimentation coefficient 152 S (strain P); of the other(s) 135 S (strain P), or 98 S (strain W), or 90 S, or 37 S, or 59 S, or 75 S (strain B). Density 1.3573-1.3645 g cm^-3 in CsCl (fixed with formaldehyde). Isoelectric point pH 6.05. A₂₆₀/A₂₈₀ ratio 1.6.

Biochemical properties

Virions contain 16 % nucleic acid; 84 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 8.5 kb. Genome of three parts; largest (or only) genome part the largest 3.355 kb (RNA-1); the 2nd largest 2.946 kb (RNA-2); the 3rd largest 2.186 kb (RNA-3). Base composition 24 % G; 26 % A; 21 % C; 29 % U.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00668 Em(40)_vi:PSVRNA3 Gb(84)_vi:PSVRNA3 Peanut stunt virus RNA 3 for 3a protein and coat protein. 6/92 2,186bp.
• D11126 Em(40)_vi:PSVJ1A Gb(84)_vi:PSVJ1A Peanut stunt virus RNA 1 complete genome. 8/92 3,355bp.
• D11127 Em(40)_vi:PSVJ2A Gb(84)_vi:PSVJ2A Peanut stunt virus RNA 2 complete genome. 5/92 2,946bp
• K03110 Em(40)_vi:CUPSVRN5 Gb(84)_vi:PSVPARNA5 Peanut stunt virus-associated RNA5 (PARNA 5). 4/90 393bp.
• X56544 Em(40)_vi:PSVCP Gb(84)_vi:PSVCP Peanut stunt virus coat protein mRNA. 8/91 986bp.
• U15728 Em(43)_vi:Ps15728 Gb(89)_vi:Psu15728 Peanut stunt virus RNA 1 complete sequence containing 1a protein mRNA, complete cds. 5/95 3,3
• U15729 Em(43)_vi:Ps15729 Gb(89)_vi:Psu15729 Peanut stunt virus RNA 2 complete sequence containing 2a protein mRNA, complete cds. 5/95 2,9
• U15730 Em(43)_vi:Ps15730 Gb(89)_vi:Psu15730 Peanut stunt virus RNA 3 complete sequence containing 3a movement protein mRNA, complete cds.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA, or satellite RNA; the former encodes the coat protein, is named RNA-4 and is of 0.986 kb, and the latter PARNA-5, a satellite RNA of 197 or 620 nucleotides.

Features of proteins

Amino acid composition: Mink (1972); Kaper and Waterworth (1981).

Cytopathology

Virions found in all parts of the host plant; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are crystals in the cytoplasm.

Taxonomy and relationships

Cucumovirus: Bromoviridae

Virus(es) with serologically related virions

Cucumber mosaic virus.

Comments and References

References

• Douine, L. and Devergne, J.C. (1978). Annls. Phytopath. 10: 79.
• Echandi, E. and Hebert, T.T. (1971). Phytopathology 61: 328.
• Fischer, H.U. and Lockhart, B.E.L. (1978). Phytopathology 68: 289.
• Kaper, J.M. and Waterworth, H.E. (1981). In: Handbook of Plant Virus Infections and Comparative Diagnosis, p. 257; ed. E. Kurstak. Elsevier/North-Holland Biomedical Press, Amsterdam.
• Karasawa, A., Nakaho, K., Kakutani, T., Minobe, Y. and Ehara, Y. (1991). Virology 185: 464.
• Kim, J.S., Lee, S.H. and Lee, M.W. (1988). Korean J. Pl. Path 4: 88.
• Milbrath, G.M. and Tolin, S.A. (1977). Pl. Dis. Reptr 61: 637.
• Miller, L.I. and Troutman, J.L. (1966). Pl. Dis. Reptr 50: 139.
• Mink, G.I. (1972). CMI/AAB Descr. Pl. Viruses No. 92, 4 pp.
• Mink, G.I., Silbernagel, M.J. and Saksena, K.N. (1969). Phytopathology 59: 1625.
• Richter, J., Proll, E. and Musil, M. (1979). Acta Virol. 23: 489.
• Schmelzer, K. (1971). CMI/AAB Descr. Pl. Viruses No. 65, 3 pp.
• Waterworth, H.E., Monroe, R.L. and Kahn, R.P. (1973). Phytopathology 63: 93.
• Xu, Z., Barnett, O.W. and Gibson, P.B. (1986). Phytopathology 76: 390.
• Zheng Q.-X. and Kung T.-H. (1984). Acta Biochim. Biophys. Sin. 16: 540.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.