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Descriptions and Lists from the VIDE Database

Peanut clump furovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.-C. Thouvenel, 1982. Revised 1984.

Nomenclature

Acronym

PCV

Strains

Indian peanut clump virus.

ICTV decimal code

27.0.1.0.003

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Arachis hypogaea - stunting, mottle, mosaic, chlorotic ringspots.
• Sorghum arundinaceum, Sorghum bicolor, Setaria italica - symptomless.

Transmission

Transmitted by a vector; a fungus; Polymyxa graminis. pH of soil affects transmission by the vector; Plasmodiophorales. Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (5-11% in Arachis hypogaea and 10% in Setaria).

Geographical distribution

Spreads in Burkina Faso, Cote d'Ivoire, India, and Senegal.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - chlorotic local lesions, ringspots and line patterns along veins, not systemic.
• Triticum aestivum, Arachis hypogaea - systemic mosaic.
• Phaseolus vulgaris, Vigna unguiculata, V. mungo, Torenia fournieri - systemic ringspots and/or mosaic.
• Nicotiana benthamiana - systemic ringspots and leaf malformation.
• N. glutinosa, Tetragonia tetragonioides - systemic ringspots.

Maintenance and propagation hosts

Phaseolus vulgaris, Nicotiana benthamiana.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), C. amaranticolor (L), Torenia fournieri (L), Nicotiana benthamiana (L) and Triticum aestivum (W).

Susceptible host species

• Antirrhinum majus
• Arachis hypogaea
• Beta vulgaris
• Capsicum frutescens
• Celosia argentea
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucumis sativus
• Cucurbita pepo
• Glycine max
• Gomphrena globosa
• Lathyrus odoratus
• Lycopersicon esculentum
• Melilotus albus
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana tabacum
• Nicotiana × edwardsonii
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Setaria italica
• Sorghum arundinaceum
• Sorghum bicolor
• Spinacia oleracea
• Tetragonia tetragonioides
• Torenia fournieri
• Triticum aestivum
• Vigna mungo
• Vigna unguiculata
• Vigna unguiculata ssp. sesquipedalis

Insusceptible host species

• Torenia fournieri

Families containing susceptible hosts

• Amaranthaceae (2/2)
• Chenopodiaceae (4/4)
• Cucurbitaceae (2/2)
• Gramineae (4/4)
• Leguminosae-Papilionoideae (8/8)
• Scrophulariaceae (2/2)
• Solanaceae (10/10)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Scrophulariaceae (1/2)

Sources of host-range data

Thouvenel et al. (1981).

Physical and biochemical properties

Properties of particles in sap

TIP: 64 °C. LIV: 28 days. DEP: log₁₀ minus 4. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains few virions.

Purification method

Thouvenel et al. (1978).

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with no clear modal length; of 190 and 245 nm; 21 nm wide. Axial canal obvious. Basic helix obvious; pitch of basic helix 2.6 nm.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 224 S; of the other(s) 183 S. Density 1.32 g cm^-3 in CsCl. Isoelectric point pH 6.45.

Biochemical properties

Virions contain 4 % nucleic acid; 96 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 12.1 kb. Genome of two parts; largest (or only) genome part the larger 6.8 kb; the 2nd largest 5.3 kb. Infectivity retained when deproteinised with phenol or detergent.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• L07269 Em(40)_vi:PNWRNAII Gb(84)_vi:PNWRNAII Peanut clump virus RNA 2 sequence, coat protein P23 and four hypothetical proteins (P39, P51,
• S62815 Em(40)_vi:S62815 3´ proximal orf (3´ end, RNA 1) peanut clump virus PCV, Genomic RNA, 316 nt. 1/94 316bp.
• X76658 Em(40)_vi:IPCVCP Gb(84)_vi:IPCVCP Indian peanut clump virus gene for coat protein. 5/94 1,129bp.
• X78602 Em(43)_vi:Pcvrna1 Gb(89)_vi:Pcvrna1 Peanut clump virus genomic RNA 1. 11/94 5,897bp.

Features of proteins

Virion protein(s) one; M_r 23000. Method of preparation: Wu and Bruening (1971).

Cytopathology

Virions found in all parts of the host plant; in cytoplasm (near the nucleus and along the plasmalemma). Inclusions absent from infected cells.

Taxonomy and relationships

Furovirus

Virus(es) with serologically related virions

Beet necrotic yellow vein, potato mop top and soil-borne wheat mosaic.

Virus(es) with serologically unrelated virions

Indian peanut clump virus nicotiana velutina mosaic, tobacco mosaic and barley stripe mosaic viruses.

Additional comments on relationships

Indian peanut clump furovirus shows no close serological relationship but has much nucleotide homology.

Comments and References

References

• Dollet, M., Fauquet, C. and Thouvenel, J.-C. (1976). Pl. Dis. Reptr 60: 1076.
• Mayo, M.A. and Reddy, D.V.R. (1985). J. gen. Virol. 66: 1347.
• Reddy, D.V.R., Robinson, D.J., Roberts, I.M. and Harrison, B.D. (1985). J. gen. Virol. 66: 2011.
• Reddy, D.V.R., Rajeshwari, R., Iizuka, N., Lesemann, D.-E., Nolt, B.L. and Goto, L. (1983). Ann. appl. Biol. 102: 305.
• Thouvenel, J.-C. and Fauquet, C. (1981). CMI/AAB Descr. Pl. Viruses No. 235, 4 pp.
• Thouvenel, J.-C., Dollet, M. and Fauquet, C. (1976). Ann. appl. Biol. 84: 311.
• Thouvenel, J.-C., Germani, G. and Pfeiffer, P. (1974). C. r. hebd. Seanc. Acad. Sci. Paris 278: 2847.
• Thouvenel, J.-C. and Fauquet, C. (1981). Ann. appl. Biol. 97: 99.
• Thouvenel, J.-C., Fauquet, C. and Dollet, M. (1978). Annls. Phytopath. 10: 93.
• Wu, G.J. and Bruening, G. (1971). Virology 46: 596.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.