Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Peanut chlorotic streak caulimovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D.V.R. Reddy, 1984. Revised 1989.

Nomenclature

Acronym

PCSV

ICTV decimal code

15.0.1.0.009

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Arachis hypogaea - chlorotic vein streaking and stunting. Symptoms slow to appear.

Transmission

Transmitted by a vector; an insect; Aphis craccivora; Aphididae. Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in India.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Canavalia ensiformis - local and systemic chlorotic spotting.
• Cyamopsis tetragonoloba - necrotic local lesions; systemic vein-clearing.
• Glycine max - systemic mosaic.
• Phaseolus vulgaris cv. Top Crop - local lesions, systemic mosaic.
• Vicia faba - chlorotic or necrotic local lesions; not systemic.
• Datura stramonium, Nicotiana rustica - chlorotic local lesions; systemic mosaic.
• Nicotiana glutinosa - systemic chlorotic spots.
• Petunia × hybrida - systemic mosaic.
• Vigna unguiculata - chlorotic local lesions with necrotic centre; not systemic.
• Spinacia oleracea - chlorotic local lesions; not systemic.

Diagnostically insusceptible host species

Chenopodium amaranticolor, C. quinoa, Brassica oleracea var. botrytis, Cassia obtusifolia, Cajanus cajan, Trifolium incarnatum, Raphanus sativus, Gomphrena globosa, Pisum sativum, Phaseolus vulgaris cv. Kintoki.

Maintenance and propagation hosts

Nicotiana benthamiana, N. × edwardsonii, N. glutinosa, N. rustica, N. clevelandii, Petunia × hybrida.

Assay hosts (Local lesions or Whole plants)

Datura stramonium (L,W), Spinacia oleracea (L), Vigna unguiculata (L), Phaseolus vulgaris cv. Top Crop (L,W).

Susceptible host species

• Arachis hypogaea
• Canavalia ensiformis
• Cyamopsis tetragonoloba
• Datura stramonium
• Glycine max
• Nicandra physalodes
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana tabacum
• Nicotiana × edwardsonii
• Petunia × hybrida
• Phaseolus vulgaris
• Spinacia oleracea
• Vicia faba
• Vigna radiata
• Vigna unguiculata

Insusceptible host species

• Brassica campestris
• Brassica juncea
• Brassica oleracea var. botrytis
• Cajanus cajan
• Cassia obtusifolia
• Chenopodium amaranticolor
• Chenopodium quinoa
• Gomphrena globosa
• Phaseolus vulgaris (cv. Kintoki)
• Pisum sativum
• Raphanus sativus
• Trifolium incarnatum
• Vicia faba

Families containing susceptible hosts

• Chenopodiaceae (1/3)
• Leguminosae-Papilionoideae (8/11)
• Solanaceae (9/9)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (2/3)
• Cruciferae (4/4)
• Leguminosae-Caesalpinioideae (1/1)
• Leguminosae-Papilionoideae (5/11)

Physical and biochemical properties

Properties of particles in sap

TIP: 80-85 °C. LIV: 3 days (at 4ºC). DEP: log₁₀ minus 3-4. Leaf sap contains few virions.

Particle morphology

Virions isometric; not enveloped; 45-50 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations. Density 1.337 g cm^-3 in CsCl.

Biochemical properties

Virions contain 16.1 % nucleic acid; 85.9 % protein; 0 % lipid.

Genome consists of DNA; double-stranded. Total genome size 7.6 kb (pairs). Genome of two parts; largest (or only) genome part 7.6 kb (pairs). Genomic nucleic acid isolated by Gardner and Shepherd (1980). Infectivity retained when deproteinised with phenol or detergent.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• U13988 Gb(84)n:PCU13988 Peanut chlorotic streak caulimovirus, complete genome. 10/94 8,174bp.

Features of proteins

Virion protein(s) three; M_r of the largest 75000. M_r of 2nd largest 44000. M_r of 3rd largest 33000.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm. Inclusions present in infected cells; are unusual in shape; a matrix; they contain virions.

Taxonomy and relationships

Caulimovirus

Virus(es) with serologically unrelated virions

Cauliflower mosaic, figwort mosaic, soybean chlorotic mottle and dahlia mosaic viruses.

Comments and References

References

• Gardner, R.C. and Shepherd, R.J. (1980). Virology 106: 159.
• Ghanekar, A.M., Reddy, D.V.R. and Iizuka, N. (1978). Indian Phytopath. 31: 118.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.