Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Pea seed-borne mosaic potyvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by P. Fry, 1980. Revised 1984.

Nomenclature

Synonyms

pea fizzle top virus, pea leaf rolling virus, pea leaf roll mosaic virus, pea leaf rolling mosaic virus.

Acronym

PSbMV

ICTV decimal code

57.0.1.0.048

Host range and symptoms

Natural host range and symptoms

Symptoms disappear soon after infection.
• Pisum sativum - transitory vein clearing, rosetting of stem and branches, leaves dark green and leaflets folded adaxially, flowers malformed and often sterile, pods small, few misshapen seeds.

Transmission

Transmitted by a vector; an insect; Acyrthosiphon pisum, Aphis craccivora, A. fabae, Dactynotus escalanti, Macrosiphum crataegarius, Rhopalosiphum padi; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; transmitted by seed (up to 100% in Pisum sativum, especially when testa is split, but little seed transmission in Vicia faba, V. narbonensis and V. pannonica).

Geographical distribution

Spreads in the Central Asian region, the Eastern Asian region, and the North American region; Australia and the UK. Found, but with no evidence of spread, in New Zealand.

Experimental host range

Many (>9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - necrotic local lesions.
• C. quinoa - chlorotic local lesions.
• Pisum sativum - systemic stunting, vein clearing, leaf rolling.
• Vicia faba - systemic vein chlorosis.

Maintenance and propagation hosts

Lathyrus odoratus, Pisum sativum, Vicia faba.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor, C. quinoa.

Susceptible host species

• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Brassica oleracea var. capitata
• Brassica oleracea var. gemmifera
• Brassica oleracea var. viridis
• Capsella bursa-pastoris
• Catharanthus roseus
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium foetidum
• Chenopodium murale
• Chenopodium quinoa
• Cucurbita maxima
• Gomphrena globosa
• Lathyrus odoratus
• Lens culinaris
• Medicago sativa
• Petunia × hybrida
• Pisum sativum
• Senecio vulgaris
• Tetragonia tetragonioides
• Vicia faba
• Vicia sativa
• Vicia villosa
• Zinnia elegans

Insusceptible host species

• Amaranthus retroflexus
• Arachis hypogaea
• Atriplex hortensis
• Avena sativa
• Beta vulgaris (sugar beet, table beet, ssp. cicla, 4 other cvs)
• Brassica campestris ssp. rapa
• Capsicum annuum
• Celosia argentea (var. cristata)
• Chenopodium hybridum
• Cucumis melo
• Cucumis sativus
• Datura stramonium
• Daucus carota
• Dianthus caryophyllus
• Glycine max
• Helianthus annuus
• Hordeum vulgare
• Hyoscyamus niger
• Lactuca sativa
• Lycopersicon esculentum
• Melilotus albus
• Nicandra physalodes
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana tabacum
• Phaseolus vulgaris
• Physalis floridana
• Raphanus sativus
• Secale cereale
• Solanum melongena
• Solanum nigrum
• Solanum tuberosum
• Spinacia oleracea
• Trifolium pratense
• Trifolium repens
• Triticum aestivum
• Vigna unguiculata
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/3)
• Apocynaceae (1/1)
• Chenopodiaceae (7/10)
• Compositae (2/4)
• Cruciferae (5/7)
• Cucurbitaceae (1/3)
• Leguminosae-Papilionoideae (7/14)
• Solanaceae (1/14)
• Tetragoniaceae (1 /1)

Families containing insusceptible hosts

• Amaranthaceae (2/3)
• Caryophyllaceae (1/1)
• Chenopodiaceae (4/10)
• Compositae (2/4)
• Cruciferae (2/7)
• Cucurbitaceae (2/3)
• Gramineae (5/5)
• Leguminosae-Papilionoideae (7/14)
• Solanaceae (13 /14)
• Umbelliferae (1/1)

Sources of host-range data

Hampton (1975).

Physical and biochemical properties

Properties of particles in sap

TIP: 55 °C. LIV: 1 days (in leaves), or 4 days (in roots). DEP: log₁₀ minus 3-4. Leaf sap contains few virions. Electron microscopy: fix with 3.5% gluraraldehyde.

Purification method

Knesek et al. (1974).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 770 nm; 12 nm wide. Axial canal obscure. Basic helix obscure; pitch of basic helix 3.4 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 154 S. Density 1.329 g cm^-3 in CsCl.

Biochemical properties

Virions contain 5.3 % nucleic acid; 94 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Base composition 22.8 % G; 44 % A; 17.6 % C; 15.6 % U.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D10453 Em(40)_vi:PSMCP Gb(84)_vi:PSMCP Pea seed-borne mosaic virus (PSbMV) genomic RNA for coat protein, partial sequence. 7/92 1,34
• D10930 Em(40)_vi:PSMCOMP1 Gb(84)_vi:PSMCOMP Pea seed-borne mosaic virus mRNA, complete cds. 4/93 9,924bp.
• U16215 Em(43)_vi:Ps16215 Gb(89)_vi:Psu16215 Pea seed-borne mosaic virus coat protein gene, partial cds. 11/94 879bp.
• X87938 Em(44)n:Pssmppoca Gb(90)_vi:Pssmppoca Pea seedborne mosaic potyvirus mRNA for RNA polymerase and capsid protein. 7/95 1,275bp.
• Z48508 Em(43)_vi:Psbmvcan Gb(89)_vi:Psbmvcan Pea seed-borne mosaic virus RNA for coat protein and polymerase (partial). 2/95 1,273bp.
• Z48509 Em(43)_vi:Psbmvger Gb(89)_vi:Psbmvger Pea seed-borne mosaic virus RNA for coat protein and polymerase (partial). 2/95 1,273bp.

Features of proteins

Virion protein(s) one; M_r 34000.

Cytopathology

Virions found in roots in cortical parenchyma and in mesophyll; in cytoplasm. Inclusions present in infected cells; are pinwheels and unusual in shape; aggregates in tonoplasts; they contain virions (in the aggregates).

Taxonomy and relationships

Potyvirus: Potyviridae

Virus(es) with serologically related virions

Bean yellow mosaic virus.

Comments and References

References

• Aapola, A.A., Knesek, J.E. and Mink, G.I. (1974). Phytopathology 64: 1003.
• Hampton, R. and Mink, G.I. (1975). CMI/AAB Descr. Pl. Viruses No. 146, 4 pp.
• Hampton, R.O., Knesek, J.E. and Mink, G.I. (1974). Phytopathology 64: 1358.
• Knesek, J.E., Mink, G.I. and Hampton, R.O. (1974). Phytopathology 64: 1076.
• Musil, M. (1966). Biol. Bratisl. 21: 133.
• Inouye, T. (1967). Ann. Phytopath. Soc. Japan 33: 38.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.