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Descriptions and Lists from the VIDE Database

Pea early browning tobravirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by B. Gerhardson and K. Ryden, 1982. Revised 1983.

Nomenclature

Synonyms

broad bean yellow band virus (Russo et al., 1984; Robinson and Harrison, 1985).

Acronym

PEBV

ICTV decimal code

72.0.1.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Pisum sativum, Phaseolus vulgaris - systemic necrotic spots, streaks and mosaic, leaves malformed, plants stunted.

Transmission

Transmitted by a vector; a nematode; Trichodorus teres, T. pachydermus in The Netherlands; T. anenome, T. primitivus, T. viruliferus in U.K; Trichodoridae. Virus transmitted by mechanical inoculation; transmitted by seed (37% in Pisum sativum cv. Rondo).

Geographical distribution

Spreads in Belgium, Italy, Morocco, the Netherlands, Sweden, and the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Gomphrena globosa - small white local lesions, not systemic.
• Chenopodium amaranticolor - small yellow local lesions, not systemic.
• C. quinoa - necrotic and chlorotic local lesions, sometimes systemic causing chlorotic spots and malformation.
• Cucumis sativus - necrotic local lesions, not systemic.
• Phaseolus vulgaris - necrotic local lesions or rings, then systemic mosaic and leaf malformation.
• Pisum sativum - diffuse chlorotic local lesions, then systemic latent infection or necrotic spots on stems and leaf veins.
• Nicotiana clevelandii - diffuse local lesions, then faint systemic mosaic.
• N. glutinosa - diffuse local lesions, then occasional systemic mosaic and chlorotic line patterns.
• N. tabacum - latent systemic infection.
• Tetragonia tetragonioides - local and systemic necrotic concentric rings.

Maintenance and propagation hosts

Phaseolus vulgaris, Pisum sativum, Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor, C. quinoa, Phaseolus vulgaris, Tetragonia tetragonioides.

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Cucumis sativus
• Datura stramonium
• Glycine max
• Gomphrena globosa
• Lathyrus odoratus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Pisum sativum
• Spinacia oleracea
• Tetragonia tetragonioides
• Trifolium hybridum
• Tropaeolum majus
• Vigna unguiculata

Insusceptible host species

• Allium cepa
• Avena sativa
• Beta vulgaris
• Brassica campestris ssp. rapa
• Hordeum vulgare
• Lactuca sativa
• Lycopersicon esculentum
• Medicago sativa
• Nicotiana glutinosa
• Solanum tuberosum
• Trifolium incarnatum
• Trifolium pratense
• Trifolium repens
• Triticum aestivum
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (4/5)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (6/11)
• Solanaceae (5/7)
• Tetragoniaceae (1/1)
• Tropaeolaceae (1/1)

Families containing insusceptible hosts

• Alliaceae (1/1)
• Chenopodiaceae (1/5)
• Compositae (1/1)
• Cruciferae (1/1)
• Gramineae (3/3)
• Leguminosae-Papilionoideae (5/11)
• Solanaceae (3/7)

Sources of host-range data

Hampton et al. (1978); Gerhardson et al. (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 74-78 °C. LIV: 183 days (or more). DEP: log₁₀ minus 5. Leaf sap contains few virions.

Purification method

Cooper and Mayo (1972); frozen sap.

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with a clear modal length; of 215 nm, or 207 nm, or 105 nm; 21 nm wide. Axial canal obvious; 4 nm in diameter. Basic helix obvious; pitch of basic helix 2.5 nm.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 286 S; of the other(s) 210 S. Density 1.31 g cm^-3 in CsCl.

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 12 kb. Genome of two parts; largest (or only) genome part the larger 7.9 kb; the 2nd largest 4.1 kb. Nucleotide sequence references: Macfarlanbe et al. (1989).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• M90705 Em(40)_vi:PEB54KA Gb(84)_vi:PEB54KA Pea early browning virus 54k gene, complete cds. 4/92 1,480bp.
• X14006 Em(40)_vi:PEBVRNA1 Gb(84)_vi:PEBVRNA1 Pea early browning virus (PEBV) genomic RNA1 for 141K, 201K, 30K and 12K proteins. 9/93 7,073
• X15883 Em(40)_vi:TOPEBVR2 Gb(84)_vi:TOPEBVR2 Pea early browning virus RNA 2 for coat protein and putative 29.6 kDa protein. 9/93 2,356bp.
• X51828 Em(40)_vi:TOPEBRN2 Gb(84)_vi:TOPEBRN2 Pea early browning virus complete RNA2 genome encoding a coat protein and two unidentified re
• X78455 Em(40)_vi:PEBVCOPR Gb(84)_vi:PEBVCOPR Pea early browning virus (isolate TPA56) RNA2 complete sequence, encoding coat protein, 9kDa 5 sequences.

Features of proteins

Virion protein(s) one; M_r 24000.

Taxonomy and relationships

Tobravirus

Differences between type strain and others

Broad bean yellow band tobravirus is a serotype of PEBV (Robinson and Harrison, 1985). It causes yellow vein banding, ringspotting and line patterns in Vicia faba in southern Italy. Its RNA-1 has a closely similar sequence to that of PEBV, although their RNA-2s differ. Pseudo-recombinants of BBYBV and PEBV are viable (B.D. Harrison, pers. comm.).

Comments and References

References

• Bos, L. and van der Want, J.P.H. (1962). Tijdschr. PltZiekt. 68: 368.
• Cooper, J.I. and Mayo, M.A. (1972). J. gen. Virol. 16: 285.
• Gerhardson, B. and Ryden, K. (1979). Phytopath. Z. 95: 93.
• Gibbs, A.J. and Harrison, B.D. (1964). Ann. appl. Biol. 54: 1.
• Hampton, R., Beczner, L., Hagedorn, D., Bos, L., Inouye, T., Barnett, O., Musil, M. and Meiners, J. (1978). Phytopathology 68: 989.
• Harrison, B.D. (1973). CMI/AAB Descr. Pl. Viruses No. 120, 4 pp.
• Macfarlane, S.A., Taylor, S.C., King, D.I., Hughes, G. and Davies, J.W. (1989). Nucl. Acids Res. 17: 2245.
• Robinson, D.J. and Harrison, B.D. (1985). J. gen. Virol. 66: 2003
• Russo, M., Gallitelli, D., Vovlas, C. and Savino, V. (1984). Ann. appl. Biol. 105: 223.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.