Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Pangola stunt fijivirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.G. Milne, 1984.

Nomenclature

Acronym

PaSV

ICTV decimal code

60.0.7.2.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Digitaria decumbens - chlorosis and reddening, leaves and flower heads malformed, curled and stunted, small veins swollen.

Transmission

Transmitted by a vector; an insect; Sogatella furcifera; Delphacidae. Transmitted in a persistent manner. Virus retained when the vector moults; multiplies in the vector; not transmitted congenitally to the progeny of the vector; not transmitted by mechanical inoculation; not transmitted by grafting; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Australia, Brazil, Fiji, Guyana, Peru, and Taiwan.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Almost all Digitaria spp. are susceptible. D. decumbens PI 111110, D. longiflora PI 299642, D. pentzii PI 299769, D. setivalvola PI 299795, D. valida PI 299875 - stunting, yellowing and twisting of leaves and inflorescences, small veins swollen, excessive tillering.

Maintenance and propagation hosts

Digitaria decumbens and other Digitaria spp.

Assay hosts (Local lesions or Whole plants)

Digitaria decumbens (W).

Susceptible host species

• Digitaria decumbens
• Digitaria longiflora (PI 299642)
• Digitaria pentzii (PI 299769)
• Digitaria setivalvola (PI 299795)
• Digitaria valida (PI 299875)

Families containing susceptible hosts

• Gramineae (5/5)

Sources of host-range data

Hunkar et al. (1974); Milne and Lovisolo (1977).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions (when prepared from whole leaves), or contains many virions (when prepared only from enations). Electron microscopy: to obtain complete or subvirions with intact spikes, use 2% aqueous UA not neutral PTA.

Purification method

Boccardo et al. (1979).

Particle morphology

Virions isometric; not enveloped; complete virions including spikes 70 nm in diameter (when negative stained and in thin section); rounded in profile, or angular in profile; with a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations (virions and spiked subvirions).

Biochemical properties

Genome consists of RNA; double-stranded; linear. Total genome size 25.188 kb. Genome of 10 parts (called S1-10); largest (or only) genome part the largest 3.405 kb; the 2nd largest 3.385 kb; the 3rd largest 3.385 kb; the 4th largest 2.6 kb; the 5th largest 2.55 kb; the 6th largest and other parts 2.49 kb (pairs; also 2.190, 1.735, 1.728 and 1.720 kb pairs). Genomic nucleic acid isolated by Boccardo et al. (1979). Additional factor required for infectivity (only complete virions are infective).

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) virion proteins probably similar in size to those of maize rough dwarf virus.

Replication

Genome replicates in cytoplasmic viroplasms. Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in phloem and phloem derived enations; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm, viroplasms, and unusual in shape; tubular structures 100 nm in diameter; they contain virions. Other cellular changes: thickening of cell walls and cell enlargement.

Taxonomy and relationships

Fijivirus: Reoviridae

Virus(es) with serologically related virions

Maize rough dwarf and rice black streaked dwarf viruses and are closely related.

Virus(es) with serologically unrelated virions

Oat sterile dwarf, sugarcane Fiji disease, rice dwarf, rice gall dwarf, rice ragged stunt and clover wound tumor viruses.

Comments and References

References

• Boccardo, G. and Milne, R.G. (1981). J. virol. Meth. 3: 109.
• Boccardo, G. and Milne, R.G. (1984). CMI/AAB Descr. Pl. Viruses No. 294, 7 pp.
• Boccardo, G., Milne, R.G. and Luisoni, E. (1979). J. gen. Virol. 45: 659.
• Conti, M. (1984). Current Topics in Vector Research; ed. K.F. Harris. Praeger Scientific, New York.
• Dirven, J.G.P. and van Hoof, E.M. (1960). Tijdsch. PlZiekt. 66: 344.
• Giannotti, J. and Milne, R.G. (1977). Virology 80: 347.
• Hunkar, A.E.S., Hung, A.T.A., Dulder, I.G., Schank, S.C., Holder, N. and Edwards, C. (1974). Trop. Agric. 52: 75.
• Milne, R.G. (1977). CMI/AAB Descr. Pl. Viruses No. 175, 4 pp.
• Milne, R.G. and Lovisolo, O. (1977). Adv. Virus Res. 21: 267.
• Milne, R.G. and Luisoni, E. (1977). Virology 80: 12.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.