Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Olive latent 2 (?) ourmiavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by M. Castellano, 1991.

Nomenclature

Acronym

OLV-2

ICTV decimal code

79.0.P.4.007

Host range and symptoms

Natural host range and symptoms

• Olea spp. - symptomless.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Found, but with no evidence of spread, in Apulia, southern Italy.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Gomphrena globosa - reddish necrotic local lesions then symptomless systemic infection.
• Chenopodium quinoa - chlorotic and necrotic local lesions.
• Nicotiana megalosiphon, N. benthamiana - mild mottling.
• Petunia × hybrida, Nicotiana clevelandii, N. debneyi, N. tabacum, Vigna radiata - symptomless.

Diagnostically insusceptible host species

Nicotiana glutinosa, N. rustica, Ocimum basilicum, Datura stramonium, Cucumis sativus, Cucurbita pepo.

Maintenance and propagation hosts

Gomphrena globosa, Nicotiana benthamiana.

Susceptible host species

• Chenopodium quinoa
• Gomphrena globosa
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana megalosiphon
• Nicotiana tabacum
• Olea europaea
• Petunia × hybrida
• Vigna radiata

Insusceptible host species

• Cucumis sativus
• Cucurbita maxima
• Cucurbita pepo
• Datura stramonium
• Nicotiana glutinosa
• Nicotiana rustica
• Ocimum basilicum

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (1/1)
• Leguminosae-Papilionoideae (1/1)
• Oleaceae (1/1)
• Solanaceae (6/9)

Families containing insusceptible hosts

• Cucurbitaceae (3/3)
• Labiatae (1/1)
• Solanaceae (3/9)

Physical and biochemical properties

Properties of particles in sap

TIP: 60 °C. LIV: more than 7 days. DEP: log₁₀ minus 6. Leaf sap contains many virions.

Purification method

Homogenize infected tissues in phosphate buffer (0.07M, pH 7.0) with 0.01M EDTA and 0.1% Na-mercaptoacetate, filter and clarify with 1 vol of 1:1 chloroform-butanol then precipitate with 10% polyethylene glycol and 1% NaCl. Purify by differential and sucrose gradient centrifugation.

Particle morphology

Virions quasi bacilliform; 37, 43, 48, and 55 nm in length; 26 nm wide.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 82 S; of the other(s) 77 S. Density 1.36 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.58.

Biochemical properties

Virions contain c. 19 % nucleic acid; 81 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 10.65 kb. Genome of four parts; largest (or only) genome part 3.3 kb; the 2nd largest 2.8 kb; the 3rd largest 2.45 kb; the 4th largest 2.1 kb.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• X76993 Em(40)_vi:OLV212 Gb(84)_vi:OLV212 Olive latent virus 2 genomic RNA-3. 2/94 2,438bp.
• X77115 Em(40)_vi:OLV2RNA4 Gb(84)_vi:OLV2RNA4 Olive latent virus 2 genomic RNA-4. 2/94 2,078bp. 2 sequences.

Features of proteins

Virion protein(s) one; M_r 24000; coat protein.

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm. Inclusions absent from infected cells. Other cellular changes: major cell organelles, except for nuclei, are affected, there are more cytoplasmic membranes and tonoplast-associated membranous vesicles.

Taxonomy and relationships

Possible ourmiavirus

Additional comments on relationships

Olive latent 2 seems not to be an ilarvirus or related to alfalfa mosaic virus. It shares most properties with pelargonium zonate spot, epirus cherry, cassava Ivorian bacilliform and melon Ourmia viruses, and hence is probably an ourmiavirus.

Comments and References

References

• Castellano, M.A., Di Franco, A. and Martelli, G.P. (1987). J. Submicrosc. Cytol. 19: 495.
• Grieco, F., Martelli, G.P., Savino, V. and Piazzolla, P. (1992). Riv. Pat. Veg.,S.V. 2: 125.
• Savino, V., Piazzolla, P., Di Franco, A. and Martelli, G.P. (1984). 5th Congr. Un. Phytopath. Medit. Cairo, Egypt.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.