Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Olive latent 1 (?) sobemovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D. Gallitelli, 1991.

Nomenclature

Acronym

OLV-1

ICTV decimal code

67.0.1.T.016

Host range and symptoms

Natural host range and symptoms

Symptoms none.
• Olea europaea - symptomless.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Found, but with no evidence of spread, in Apulia, southern Italy.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, but no systemic infection, except in Nicotiana benthamiana.

Diagnostically susceptible host species and symptoms

• Nicotiana benthamiana - systemic mosaic.
• Chenopodium amaranticolor, C. quinoa, Cucumis sativus, Nicotiana clevelandii, N. glutinosa, N. megalosiphon, N. tabacum, Gomphrena globosa - necrotic local lesions; no systemic infection.

Diagnostically insusceptible host species

Datura stramonium, Nicotiana rustica, Petunia × hybrida.

Maintenance and propagation hosts

Nicotiana benthamiana.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), Nicotiana benthamiana (W), N. clevelandii (L), Cucumis sativus (L), Gomphrena globosa (L).

Susceptible host species

• Celosia cristata
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucumis sativus
• Cucurbita pepo
• Gomphrena globosa
• Momordica balsamina
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana tabacum
• Olea europaea
• Phaseolus vulgaris
• Vigna unguiculata

Insusceptible host species

• Datura stramonium
• Nicotiana rustica
• Petunia × hybrida

Families containing susceptible hosts

• Amaranthaceae (2/2)
• Chenopodiaceae (2/2)
• Cucurbitaceae (3/3)
• Leguminosae-Papilionoideae (2/2)
• Oleaceae (1/1)
• Solanaceae (5/8)

Families containing insusceptible hosts

• Solanaceae (3/8)

Sources of host-range data

Gallitelli and Savino (1985).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains many virions.

Purification method

Gallitelli and Savino (1985).

Particle morphology

Virions isometric; 30 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient of the fastest 111 S. Density 1.314 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.64.

Biochemical properties

Virions contain c. 17 % nucleic acid; 83 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 4.1 kb. Genome unipartite; largest (or only) genome part 4.1 kb. Genomic nucleic acid isolated by Gallitelli: phenol-SDS. Infectivity retained when deproteinised with proteases; retained when deproteinised with phenol or detergent.

NCBI sequence data (Entrez search)

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 32000; coat protein. Method of preparation: Laemmli (1970).

Cytopathology

Virions found in leaves and mesophyll; in cytoplasm. Inclusions present in infected cells; are crystals in the cytoplasm and unusual in shape; vesiculated bodies and tubules; they contain virions. Other cellular changes: those described by Castellano et al. (1987).

Taxonomy and relationships

Possible Sobemovirus

Comments and References

References

• Castellano, M.A., Di Franco, A. and Martelli, G.P. (1987). J. Submicrosc. Cytol. 19: 495.
• Gallitelli, D. and Savino, V. (1985). Ann. appl. Biol. 106: 295.
• Laemmli, U.K. (1970). Nature, Lond. 227: 680.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.