Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Olive latent ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by D. Gallitelli, 1987.

Nomenclature

Acronym

OLRSV

ICTV decimal code

18.0.3.0.022

Host range and symptoms

Natural host range and symptoms

• Olea spp. - symptomless.

Transmission

Virus transmitted by mechanical inoculation; transmitted by grafting.

Geographical distribution

Spreads in Italy (Lozio, central Italy).

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. amaranticolor - chlorotic or necrotic local lesions, systemic mosaic, apical necrosis.
• Gomphrena globosa - red-rimmed local lesions, tip leaves malformed.

Diagnostically insusceptible host species

Nicotiana tabacum cvs Samsun, White Barley, N. glutinosa, Vigna radiata.

Maintenance and propagation hosts

Chenopodium quinoa, Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (W), Chenopodium amaranticolor (W), Cucumis sativus cv. Markerer (W).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucumis sativus
• Gomphrena globosa
• Nicotiana clevelandii
• Olea europaea
• Petunia × hybrida
• Phaseolus vulgaris

Insusceptible host species

• Nicotiana glutinosa
• Nicotiana tabacum
• Vigna radiata

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (2/2)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (1/2)
• Oleaceae (1/1)
• Solanaceae (2/4)

Families containing insusceptible hosts

• Leguminosae-Papilionoideae (1/2)
• Solanaceae (2/4)

Sources of host-range data

Savino et al. (1983).

Physical and biochemical properties

Properties of particles in sap

TIP: 60 °C. LIV: 15 days. DEP: log₁₀ minus 3.

Purification method

Gallitelli: homogenise symptom bearing Chenopodium quinoa leaves in 0.1M neutral phosphate buffer. Clarify the sap with Mg-activated bentonite. Purify by two cycles of centrifugation.

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 132 S (B); of the other(s) 97 S (M), or 52 S (T). Density 1.52 g cm^-3 in CsCl (B₂), or 1.51 g cm^-3 in CsCl (B₁), or 1.43 g cm^-3 in CsCl (M), or 1.29 g cm^-3 in CsCl (T). A₂₆₀/A₂₈₀ ratio 1.8 (B), or 1.51 (M), or 1.03 (T).

Biochemical properties

Virions contain 45 % nucleic acid (B₂), or 43 % nucleic acid (B₁), or 32 % nucleic acid (M), or 0 % nucleic acid (T); 55 % protein (B₂), or 57 % protein (B₁), or 68 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded. Total genome size 11.4 kb. Genome of two parts; largest (or only) genome part the larger 7.5 kb; the 2nd largest 3.9 kb. Genomic nucleic acid isolated by Gallitelli; best means by phenol - SDS. Infectivity retained when deproteinised with phenol or detergent. Additional factor not required for infectivity.

Features of proteins

Virion protein(s) one; M_r 58000; a polymer of a monomeric form of 16kDa. Method of preparation: Gallitelli; boil for 3 minutes in 125mM Tris-Cl pH 6.8 containing 1% SDS, 2% beta-mercaptoethanol, 6M urea. Virion proteins not glycosylated.

Cytopathology

Virions found in leaves mesophyll and epidermis; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are crystals in the cytoplasm and membranous bodies; they contain virions. Other cellular changes: clumping of chloroplasts and finger-like outgrowths of the cell.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, artichoke Italian latent, artichoke yellow ringspot, artichoke vein banding, cherry leaf roll, cherry rasp leaf, chicory yellow mottle, cacao necrosis, grapevine Bulgarian latent, grapevine fanleaf, grapevine chrome mosaic, myrobalan latent ringspot raspberry ringspot, strawberry latent ringspot, tobacco ringspot, tomato black ring and tomato ringspot viruses.

Comments and References

References

• Gallitelli, D., Martelli, G.P. and Savino, V. (1985). AAB Descr. Pl. Viruses No. 301, 4 pp.
• Savino, V., Gallitelli, D. and Barba, M. (1983). Ann. appl. Biol. 103: 243.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.