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Descriptions and Lists from the VIDE Database

Nicotiana velutina mosaic (?) furovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.W. Randles, 1985.

Nomenclature

Acronym

NVMV

ICTV decimal code

27.0.1.T.DE.2

Host range and symptoms

Natural host range and symptoms

• Nicotiana velutina - bright yellow mosaic.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by seed (up to 72% in Nicotiana glutinosa).

Geographical distribution

Spreads in Australia.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Nicotiana glutinosa - systemic chlorosis.
• N. tabacum - etched ringspots, then mild systemic mottling.
• N. debneyi - necrosis; then systemic mosaic and malformation.
• Chenopodium amaranticolor, C. quinoa - chlorotic local lesions; not systemic.
• Beta macrocarpa - local lesions; systemic mosaic.
• Gomphrena globosa - necrotic ringspots; systemic chlorosis.

Diagnostically insusceptible host species

Tetragonia tetragonioides.

Maintenance and propagation hosts

Nicotiana debneyi.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L).

Susceptible host species

• Beta macrocarpa
• Chenopodium amaranticolor
• Chenopodium quinoa
• Gomphrena globosa
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Nicotiana velutina
• Spinacia oleracea

Insusceptible host species

• Cucumis sativus
• Dianthus barbatus
• Lathyrus odoratus
• Phaseolus vulgaris
• Tetragonia tetragonioides
• Triticum aestivum
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (4/4)
• Solanaceae (8/8)

Families containing insusceptible hosts

• Caryophyllaceae (1/1)
• Cucurbitaceae (1/1)
• Gramineae (1/1)
• Leguminosae-Papilionoideae (3/3)
• Tetragoniaceae (1/1)

Physical and biochemical properties

Properties of particles in sap

TIP: 60-70 °C. LIV: 1-4 days. DEP: log₁₀ minus 1-2. Leaf sap contains few virions.

Purification method

Randles (1978).

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with no clear modal length; of 125-150 nm (range 100-175 nm); 18 nm wide. Axial canal obvious. Basic helix obvious; pitch of basic helix 2.9 nm.

Physical properties

Eight sedimenting components in purified preparations (a broad band (aggregates, not in separate components)); sedimentation coefficient of the fastest 155 S (and greater).

Biochemical properties

Genome consists of RNA; single-stranded; linear. Total genome size 11 kb. Genome of two parts; largest (or only) genome part the larger 8 kb (RNA 1); the 2nd largest 3 kb (RNA 2). Infectivity retained when deproteinised with phenol or detergent. Nucleotide sequence references: Randles and Rohde (1990).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00906 Em(40)_vi:NVMV2 Gb(84)_vi:NVMV2 Nicotiana velutina mosaic virus RNA2. 2/93 2,325bp. 1 sequence.

Features of the genome

Features of the genome: RNA 2 contains 4 ORF's; ORF 1 = protein 20K, ORF 2 = protein 39K, ORF 3 = protein 13K and one ORF is incomplete.

Features of proteins

M_r 21400. Method of preparation: Randles et al. (1976).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in locally or systemically infected tissue in thin sections. Inclusions absent from infected cells.

Taxonomy and relationships

Possible Furovirus

Virus(es) with serologically unrelated virions

Potato mop top, soil-borne wheat mosaic, tobacco mosaic, beet necrotic yellow vein, barley stripe mosaic and tobacco rattle viruses viruses.

Additional comments on relationships

CDNA clones did not hybridize with tobacco mosaic, frangipani mosaic, odontoglossum ringspot, tomato mosaic, sunn-hemp mosaic, cucumber green mottle mosaic and Kyuri green mottle mosaic tobamoviruses or beet necrotic yellow vein and peanut clump furoviruses. Although the virus has a divided genome with two RNA's of a size comparable to those of furoviruses, its unique biological properties and lack of serological relationship with furoviruses suggest that it has no clear relationship to any recognised taxonomic group of plant viruses.

Comments and References

References

• Randles, J.W. (1978). CMI/AAB Descr. Pl. Viruses No. 189, 4 pp.
• Randles, J.W. and Rohde, W. (1990). J. gen. Virol. 71: 1019.
• Randles, J.W., Harrison, B.D. and Roberts, I.M. (1976). Ann. appl. Biol. 84: 193.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.