Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Nerine X potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, 1986.

Nomenclature

Synonyms

agapanthus X virus.

Acronym

NVX

Strains

Agapanthus, nerine.

ICTV decimal code

56.0.1.0.012

Host range and symptoms

Natural host range and symptoms

• Nerine sarniensis (nerine strain) - symptomless.
• Agapanthus praecox ssp. orientalis (agapanthus strain) - symptomless.

Transmission

Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in the Netherlands and the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa - chlorotic or necrotic local lesions, then systemic chlorosis.
• Chenopodium amaranticolor, C. murale, C. capitatum, Gomphrena globosa and Amaranthus retroflexus - chlorotic local lesions, not systemic.
• Tetragonia tetragonioides - etched chlorotic local lesions, not systemic. All tests done with the agapanthus strain.

Diagnostically insusceptible host species

Lilium cv. Enchantment, Narcissus pseudonarcissus, Solanum tuberosum, Nicotiana clevelandii, Hippeastrum hybridum.

Maintenance and propagation hosts

Chenopodium quinoa.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L-W), Chenopodium amaranticolor (L) and Chenopodium murale (L).

Susceptible host species

• Agapanthus praecox ssp. orientalis
• Amaranthus retroflexus
• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Gomphrena globosa
• Lycopersicon esculentum
• Nerine sarniensis
• Nicandra physalodes
• Nicotiana debneyi
• Tetragonia tetragonioides
• Trifolium incarnatum

Insusceptible host species

• Allium cepa
• Arachis hypogaea
• Belamcanda chinensis
• Brassica campestris ssp. pekinensis
• Cajanus cajan
• Calendula officinalis
• Capsicum annuum
• Cucumis sativus
• Dahlia pinnata
• Datura stramonium
• Helianthus annuus
• Hippeastrum hybridum
• Lactuca sativa
• Lilium (cv. Enchantment)
• Lilium formosanum
• Montia perfoliata
• Narcissus pseudonarcissus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Solanum demissum
• Solanum tuberosum
• Verbesina encelioides
• Vicia faba
• Zinnia elegans

Families containing susceptible hosts

• Alliaceae (1/2)
• Amaranthaceae (2/2)
• Amaryllidaceae (1/3)
• Chenopodiaceae (4/4)
• Leguminosae-Papilionoideae (1/5)
• Solanaceae (3/11)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Alliaceae (1/2)
• Amaryllidaceae (2/3)
• Compositae (6/6)
• Cruciferae (1/1)
• Cucurbitaceae (1/1)
• Iridaceae (1/1)
• Leguminosae-Papilionoideae (4/5)
• Liliaceae (2/2)
• Portulacaceae (1/1)
• Solanaceae (8 /11)

Sources of host-range data

Phillips and Brunt (1980).

Physical and biochemical properties

Properties of particles in sap

TIP: 95-100 °C. LIV: 365 days (at least). DEP: log₁₀ minus 7 (all tests in Chenopodium quinoa sap; agapanthus strain). Leaf sap contains few virions.

Purification method

Maat (1976), for nerine strain; Phillips and Brunt (1980), agapanthus strain.

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 540 nm; 11 nm wide. Axial canal obscure. Basic helix obscure.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 120 S. Density 1.31 g cm^-3 in CsCl (unfixed). A₂₆₀/A₂₈₀ ratio 1.18-1.4.

Biochemical properties

Virions contain 5 % nucleic acid; 95 % protein.

Genome consists of RNA; single-stranded. Total genome size 7 kb. Genome unipartite; largest (or only) genome part 7 kb. Genomic nucleic acid isolated by Brunt et al. (1980).

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 25600; coat protein. Method of preparation: Brunt et al. (1975).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant.

Taxonomy and relationships

Potexvirus

Virus(es) with serologically related virions

Commelina X (SDI 8), hydrangea ringspot (SDI 9), potato X (SDI 5) and viola mottle (SDI 9) viruses.

Virus(es) with serologically unrelated virions

Bamboo mosaic, cactus X, cymbidium mosaic, lily X, narcissus mosaic, papaya mosaic (type strain and ullucus strain), potato aucuba mosaic and white clover mosaic viruses.

Best tests for diagnosis

ISEM with `decoration'.

Comments and References

General comments

The type strain from Nerine sarniensis is isolated and maintained in test plants with difficulty. Most data in this description are from work with the strain from Agapanthus praecox ssp. orientalis, which is serologically indistinguishable from the type strain, but is readily isolated, reaches a high concentration in test plants and is very stable in vitro.

References

• Brunt, A.A., Barton, R.J., Phillips, S. and Lana, A.O. (1980). Ann. appl. Biol. 96: 37.
• Brunt, A.A., Barton, R.J., Tremaine, J.H. and Stace-Smith, R. (1975). J. gen. Virol. 27: 101.
• Brunt, A.A., Hollings, M. and Stone, O.M. (1970). Rep. Glasshouse Crops Res. Inst. 1969, p. 138.
• Maat, D.Z. (1976). Neth. J. Pl. Path. 82: 95.
• Phillips, S. and Brunt, A.A. (1980). Acta Hort. 110: 65.
• Phillips, S. and Brunt, A.A. (1988). CMI/AAB Descr. Pl. Viruses No. 336, 3 pp.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.