Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Nerine latent carlavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, 1986.

Nomenclature

Synonyms

hippeastrum latent virus.

Acronym

NeLV

Strains

nerine latent carlavirus, hippeastrum strain.

ICTV decimal code

14.0.1.0.021

Host range and symptoms

Natural host range and symptoms

• Nerine bowdenii, Hippeastrum hybridum - symptomless.

Transmission

Transmitted by a vector; an insect; Myzus persicae; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in the Netherlands and the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. murale and C. quinoa - chlorotic local lesions, not systemic.
• Gomphrena globosa - pinkish chlorotic local lesions only.
• Nicotiana clevelandii - faint chlorotic local lesions, then systemic mild veinal chlorosis.
• Datura stramonium - chlorotic local lesions, not systemic.

Diagnostically insusceptible host species

Hyoscyamus niger, Trifolium incarnatum, Tetragonia tetragonioides.

Maintenance and propagation hosts

Nicotiana clevelandii, Chenopodium quinoa, Nicotiana glutinosa × N. clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa, Chenopodium murale and Nicotiana clevelandii.

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Datura stramonium
• Gomphrena globosa
• Hippeastrum hybridum
• Nerine bowdenii
• Nicotiana clevelandii
• Nicotiana glutinosa × N. clevelandii

Insusceptible host species

• Amaranthus caudatus
• Belamcanda chinensis
• Brassica campestris ssp. pekinensis
• Calendula officinalis
• Capsicum annuum
• Cucumis melo
• Cucumis sativus
• Cucurbita pepo
• Dahlia pinnata
• Dianthus caryophyllus
• Emilia sagittata
• Fagopyrum esculentum
• Helianthus annuus
• Hordeum vulgare
• Hyoscyamus niger
• Lactuca sativa
• Lycopersicon esculentum
• Momordica balsamina
• Nicotiana benthamiana
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Pisum sativum
• Tetragonia tetragonioides
• Trifolium incarnatum
• Trifolium repens
• Tropaeolum majus
• Vigna unguiculata
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/2)
• Amaryllidaceae (2/2)
• Chenopodiaceae (4/4)
• Solanaceae (3/12)

Families containing insusceptible hosts

• Amaranthaceae (1/2)
• Caryophyllaceae (1/1)
• Compositae (5/5)
• Cruciferae (1/1)
• Cucurbitaceae (4/4)
• Gramineae (2/2)
• Iridaceae (1/1)
• Leguminosae-Papilionoideae (5/5)
• Polygonaceae (1/1)
• Solanaceae (9 /12)
• Tetragoniaceae (1/1)
• Tropaeolaceae (1/1)

Sources of host-range data

Brolman-Hupkes (1975); Maat et al. (1978).

Physical and biochemical properties

Properties of particles in sap

TIP: 70-80 °C. LIV: 1-2 days. DEP: log₁₀ minus 3-4. Leaf sap contains many virions. Electron microscopy: ISEM may be necessary to detect virions in sap.

Purification method

Maat et al. (1978).

Particle morphology

Virions filamentous; not enveloped; usually straight; with a clear modal length; of 665 nm; 13 nm wide. Axial canal obscure. Basic helix obscure.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 155 S. Density 1.298 g cm^-3 in CsCl (unfixed). A₂₆₀/A₂₈₀ ratio 1.08-1.19.

Biochemical properties

Virions contain 5 % nucleic acid; 95 % protein; 0 % lipid.

Features of proteins

Virion protein(s) one; M_r 32600; coat protein. Method of preparation: Brunt et al. (1975).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm.

Taxonomy and relationships

Carlavirus

Virus(es) with serologically related virions

Carnation latent (SDI 3) and chrysanthemum B (SDI 10) viruses.

Virus(es) with serologically unrelated virions

Red clover vein mosaic, cowpea mild mottle, elderberry, honeysuckle latent, lily symptomless, narcissus latent, potato M and potato S viruses.

Best tests for diagnosis

ISEM with `decoration' best for distinguishing from similar viruses. NLV together with hippeastrum mosaic potyvirus is common in Hippeastrum hybridum varieties, but the two viruses can be separated and identified by inoculation to Gomphrena globosa and Hyoscyamus niger.

Comments and References

References

• Brolman-Hupkes (1975). Neth. J. Pl. Path. 81: 226.
• Brunt, A.A., Barton, R.J., Tremaine, J.H. and Stace-Smith, R. (1975). J. gen. Virol. 27: 101.
• Brunt, A.A., Hollings, M. and Stone, O.M. (1970). Rep. Glasshouse Crops Res. Inst. 1969, p. 138.
• Maat, D.Z., Huttinga, H. and Hakkaart, F.A. (1978). Neth. J. Pl. Path. 84: 47.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.