Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Narcissus tip necrosis (?) carmovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, 1992.

Nomenclature

Acronym

NTNV

ICTV decimal code

74.0.2.T.018

Host range and symptoms

Natural host range and symptoms

Symptoms vary seasonally.
• Narcissus pseudonarcissus, N. tazetta, N. poeticus - usually symptomless; sometimes general leaf chlorosis, elongate chlorotic areas near leaf tips, later becoming brown and necrotic, premature leaf senescence.

Transmission

Virus transmitted by mechanical inoculation; not transmitted by grafting.

Geographical distribution

Spreads in the Netherlands and the UK.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show small chlorotic local lesions 2mm; no systemic infection.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, C. murale - chlorotic local lesions; no systemic infection.
• Tetragonia tetragonioides - chlorotic local lesions or green rings; no systemic infection.
• Chenopodium amaranticolor - few pin prick chlorotic local lesions.
• Chenopodium capitatum, Nicotiana benthamiana - symptomless local infection only.

Diagnostically insusceptible host species

Nicotiana clevelandii, N. tabacum, Belamcanda chinensis.

Maintenance and propagation hosts

Chenopodium quinoa, C. capitatum, C. murale, Tetragonia tetragonioides, Narcissus pseudonarcissus cvs Barrett Browning, Goblet, Redmarley.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Narcissus poeticus
• Narcissus pseudonarcissus
• Narcissus tazetta
• Nicotiana benthamiana
• Tetragonia tetragonioides

Insusceptible host species

• Avena sativa
• Belamcanda chinensis
• Dahlia pinnata
• Dianthus barbatus
• Gomphrena globosa
• Helianthus annuus
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana tabacum
• Phaseolus vulgaris
• Solanum demissum × S. tuberosum
• Spinacia oleracea
• Trifolium incarnatum
• Triticum aestivum
• Vicia faba
• Vigna unguiculata ssp. sesquipedalis

Families containing susceptible hosts

• Amaryllidaceae (3/3)
• Chenopodiaceae (4/5)
• Solanaceae (1/6)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Caryophyllaceae (1/1)
• Chenopodiaceae (1/5)
• Compositae (2/2)
• Gramineae (2/2)
• Iridaceae (1/1)
• Leguminosae-Papilionoideae (4/4)
• Solanaceae (5/6)

Comments on host-range

Hosts become infected only by inoculation with purified virus preparations, never directly from infected Narcissus sap.

Sources of host-range data

S. Phillips and A. Brunt (unpublished data); Mowat et al. (1977).

Physical and biochemical properties

Properties of particles in sap

TIP: 75 °C. Leaf sap contains few virions. Electron microscopy: ISEM.

Purification method

Mowat et al. (1977).

Particle morphology

Virions isometric; not enveloped; 30.7 nm in diameter; neither clearly angular or nor rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 123 S. Density 1.356 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.5-1.6.

Biochemical properties

Virions contain 16-20 % nucleic acid; 80-84 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 4.85 kb. Genome unipartite; largest (or only) genome part 4.85 kb. Genomic nucleic acid isolated by Mowat et al. (1977); Murant et al. (1972).

Features of proteins

Virion protein(s) one (plus a fragment); M_r 42000 (plus 39000); coat protein. Method of preparation: Mayo et al. (1974); Brunt et al. (1975). Virion proteins not glycosylated. Virion proteins not phosphorylated.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll, epidermis and meristems; in cytoplasm.

Taxonomy and relationships

Possible Carmovirus: Tombusviridae

Virus(es) with serologically unrelated virions

Carnation mottle, elderberry latent, pelargonium flower break, saguaro cactus and turnip crinkle viruses.

Comments and References

References

• Asjes, C.J. (1972). Jversl. Lab. Bloemboll. Lisse, 1970-1971 p. 45.
• Asjes, C.J. (1974). Jversl. Lab. Bloemboll., Lisse, 1973, p. 46.
• Brunt, A.A. (1975). Rep. Glasshouse Crops Res. Inst. 1974, p. 116.
• Brunt, A.A., Barton, R.J., Tremaine., J.H. and Stace-Smith, R. (1975). J. gen. Virol. 27: 101.
• Mayo, M.A., Robinson, D.J. and Perombelon, M.C.M. (1974). J. gen. Microbiol. 85: 121.
• Mowat, W.P. and Hutcheson, A.M. (1974). Rep. Scottish Hort. Res. Inst. 1973, p. 61.
• Mowat, W.P., Asjes, C.J. and Brunt, A.A. (1976). CMI/AAB Descr. Pl. Viruses No. 166, 4 pp.
• Mowat, W.P., Asjes, C.J. and Brunt, A.A. (1977). Ann. appl. Biol. 86: 189.
• Murant, A.F., Mayo, M.A., Harrison, B.D. and Goold, R.A. (1972). J. gen. Virol. 16: 327.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.