Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Narcissus mosaic potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by W.P. Mowat, 1986.

Nomenclature

Acronym

NMV

Strains

Lychnis potexvirus (R.I. Hamilton in Koenig, 1985).

ICTV decimal code

56.0.1.0.011

Host range and symptoms

Natural host range and symptoms

• Narcissus pseudonarcissus - symptomless infection.

Transmission

Virus transmitted by mechanical inoculation; not transmitted by contact between plants (between narcissus plants); not transmitted by seed.

Geographical distribution

Probably distributed worldwide (in all countries which have imported narcissus and nerine from Europe). Spreads in particularly the Netherlands and the UK.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - chlorotic local lesions.
• Nicotiana clevelandii - symptomless systemic infection.
• Gomphrena globosa - necrotic local lesions.

Diagnostically insusceptible host species

Nicotiana tabacum cv. White Burley, N. glutinosa, Brassica campestris ssp. pekinensis.

Maintenance and propagation hosts

Chenopodium quinoa, Nicotiana clevelandii (not infected by some isolates).

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L).

Susceptible host species

• Apium graveolens
• Atriplex hortensis
• Catharanthus roseus
• Chenopodium amaranticolor
• Chenopodium quinoa
• Datura stramonium
• Glycine max
• Gomphrena globosa
• Lactuca sativa
• Medicago sativa
• Narcissus pseudonarcissus
• Nerine bowdenii
• Nicandra physalodes
• Nicotiana clevelandii
• Petunia × hybrida
• Pisum sativum
• Tetragonia tetragonioides
• Trifolium pratense
• Trifolium repens

Insusceptible host species

• Allium cepa
• Antirrhinum majus
• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Brassica juncea
• Cucumis sativus
• Fagopyrum esculentum
• Lilium formosanum
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana rustica
• Nicotiana tabacum
• Phaseolus vulgaris
• Solanum tuberosum
• Vicia faba
• Zea mays

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Amaryllidaceae (2/2)
• Apocynaceae (1/1)
• Chenopodiaceae (3/4)
• Compositae (1/1)
• Leguminosae-Papilionoideae (5/7)
• Solanaceae (4/9)
• Tetragoniaceae (1/1)
• Umbelliferae (1 /1)

Families containing insusceptible hosts

• Alliaceae (1/1)
• Chenopodiaceae (1/4)
• Cruciferae (2/2)
• Cucurbitaceae (1/1)
• Gramineae (1/1)
• Leguminosae-Papilionoideae (2/7)
• Liliaceae (1/1)
• Polygonaceae (1/1)
• Scrophulariaceae (1 /1)
• Solanaceae (5/9)

Sources of host-range data

Brunt (1966).

Physical and biochemical properties

Properties of particles in sap

TIP: 75 °C. LIV: 85 days (and more). DEP: log₁₀ minus 6. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Mowat (1971).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 550 nm; 13-14 nm wide. Axial canal obscure. Basic helix obscure; pitch of basic helix 3.4 nm.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest, the 550 nm virions 114 S.

Biochemical properties

Virions contain 6 % nucleic acid; 94 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 6.955 kb. Genome unipartite; largest (or only) genome part 6.955 kb. Genomic nucleic acid isolated by Short and Davies (1983).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00405 Em(40)_vi:NMV Narcissus mosaic virus (NMV), complete genome. 8/90 6,955bp
• D13747 Em(40)_vi:MNCCGAA Gb(84)_vi:MNCCGAA Narcissus mosaic virus (NMV), complete genome. 11/92 6,955bp. 2 sequences.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA. Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 24000. Method of preparation: Robinson et al. (1975). Amino acid composition: Short (1982).

Virus-coded non-virion proteins identified by genomic sequence analysis; three proteins found. M_r of the largest 116000. M_r of 2nd largest 66000. M_r of 3rd 44000.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in epidermis; in cytoplasm, in nuclei, and in cell vacuoles. Inclusions present in infected cells; are unusual in shape; spindle-shaped; they contain virions.

Taxonomy and relationships

Potexvirus

Virus(es) with serologically unrelated virions

Tulip X virus.

Comments and References

References

• Bancroft, J.B., Hills, G.J. and Richardson, J.F. (1980). J. gen. Virol. 50: 451.
• Brunt, A.A. (1966). Ann. appl. Biol. 58: 13.
• Koenig, R. (1985). Acta Hort. 164: 21.
• Mowat, W.P. (1971). CMI/AAB Descr. Pl. Viruses No. 45, 3 pp.
• Robinson, D.J., Hutcheson, A., Tollin, P. and Wilson, H.R. (1975). J. gen. Virol. 29: 325.
• Short, M.N. (1982). Ph.D. Thesis, University of East Anglia, U.K.
• Short, M.N. and Davis, J.W. (1983). Bioscience Rep. 3: 837.
• Van Slogteren, D.H.M. and de Bruyn Ouboter, M.P. (1946). Daffodil Tulip Yrbk. 12: 3.
• Zuidema, D., Linthorst, H.J.M., Huisman, M.J., Asjes, C.J. and Bol, J.F. (1989). J. gen. Virol. 70: 267.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.