Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Myrobalan latent ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R. Delbos, 1991.

Nomenclature

Acronym

MLRSV

ICTV decimal code

18.0.3.0.021

Host range and symptoms

Natural host range and symptoms

• Prunus avium - enations on undersides of leaves.
• Prunus persica - severe rosetting.

Transmission

Transmitted by a vector; a nematode (probably). Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants.

Geographical distribution

Found, but with no evidence of spread, in France.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa - local lesions; systemic necrosis.
• Nicotiana tabacum - necrotic spots and ringspots.

Maintenance and propagation hosts

Chenopodium quinoa, Nicotiana clevelandii, Pisum sativum.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), Nicotiana tabacum (L and W).

Susceptible host species

• Chenopodium quinoa
• Nicotiana clevelandii
• Nicotiana tabacum
• Pisum sativum
• Prunus avium
• Prunus cerasifera
• Prunus persica

Families containing susceptible hosts

• Chenopodiaceae (1/1)
• Leguminosae-Papilionoideae (1/1)
• Rosaceae (3/3)
• Solanaceae (2/2)

Physical and biochemical properties

Properties of particles in sap

TIP: 72 °C. LIV: 100 days. DEP: log₁₀ minus 4.

Purification method

Clarify with butanol:chloroform, followed by differential centrifugation.

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 115 S (B); of the other(s) 105 S (M). Density 1.5 g cm^-3 in CsCl (B), or 1.46 g cm^-3 in CsCl (M). A₂₆₀/A₂₈₀ ratio 1.73 (B), or 1.65 (M).

Biochemical properties

Virions contain 38 % nucleic acid (B), or 28 % nucleic acid (M), or 0 % nucleic acid (T); 62 % protein (B), or 72 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded; linear. Total genome size 12.7 kb. Genome of two parts; largest (or only) genome part the larger 7 kb; the 2nd largest 5.7 kb. Genomic nucleic acid isolated by Dunez et al. (1976): SDS followed by sucrose gradient centrifugation and ethanol precipitation. Infectivity lost when deproteinised with proteases; retained when deproteinised with phenol or detergent.

Features of the genome

Non-genomic nucleic acid found in the virions; is of some isolates satellite RNA.

Features of proteins

Virion protein(s) one; M_r 53000; capsid protein. Virion proteins not glycosylated. Virion proteins not phosphorylated.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves and roots.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically related virions

Tomato black ring, but distantly.

Virus(es) with serologically unrelated virions

Arabis mosaic, artichoke Italian latent, cherry leaf roll, raspberry ringspot, strawberry latent ringspot, tobacco ringspot and tomato ringspot viruses (Delbos et al., 1976).

Comments and References

References

• Delbos, R., Dunez, J., Barrau, J. and Fisac, R. (1976). Annls. Microbiol. (Paris) 127A: 101.
• Dunez, J. and Delbos, R. (1976). Mitt. Biol. Bund. fur Land. Fortw., Berlin 170: 9.
• Dunez, J., Delbos, R. and Dupont (1975). CMI/AAB Descr. Pl. Viruses No. 160.
• Dunez, J., Delbos, R., Desvignes, J.C., Marenaud, C., Kuszala, J. and Vuittenez, A. (1971). Annls. Phytopath., p. 117.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.