Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Mulberry ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt and T. Tsuchizaki, 1987.

Nomenclature

Acronym

MRSV.

ICTV decimal code

18.0.3.0.020

Host range and symptoms

Natural host range and symptoms

Symptoms persist and vary seasonally.
• Morus alba - mosaic and ringspotting, enations with some isolates.

Transmission

Transmitted by a vector; a nematode; Longidorus martini; Dorylamidae. Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; transmitted by seed (c. 10% in Glycine max).

Ecology and control

Studies reported by Yagita and Komuro (1972).

Geographical distribution

Spreads in Japan.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Morus alba - mosaic, ringspotting, enations with some isolates.
• Glycine max, Pisum sativum - systemic chlorotic spotting and mosaic.
• Vigna unguiculata - necrotic spots or ringspots; systemic mosaic and/or necrosis of leaves and stems.
• Chenopodium quinoa - faint chlorotic local lesions; then systemic mosaic.

Diagnostically insusceptible host species

Nicotiana tabacum, Vicia faba, Lycopersicon esculentum, Chenopodium amaranticolor, Cucumis sativus.

Maintenance and propagation hosts

Vigna unguiculata, Glycine max.

Assay hosts (Local lesions or Whole plants)

Vigna unguiculata (L).

Susceptible host species

• Chenopodium murale
• Chenopodium quinoa
• Glycine max
• Morus alba
• Nicotiana clevelandii
• Pisum sativum
• Sesamum indicum
• Vigna unguiculata
• Vigna unguiculata ssp. sesquipedalis

Insusceptible host species

• Abelmoschus esculentus
• Amaranthus retroflexus
• Capsicum annuum
• Catharanthus roseus
• Chenopodium album
• Chenopodium amaranticolor
• Cucumis sativus
• Daucus carota
• Galinsoga parviflora
• Gomphrena globosa
• Lycopersicon esculentum
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Solanum melongena
• Solanum nigrum
• Spinacia oleracea
• Tetragonia tetragonioides
• Vicia faba
• Zea mays
• Zinnia elegans

Families containing susceptible hosts

• Chenopodiaceae (2/5)
• Leguminosae-Papilionoideae (4/6)
• Moraceae (1/1)
• Pedaliaceae (1/1)
• Solanaceae (1/7)

Families containing insusceptible hosts

• Amaranthaceae (2/2)
• Apocynaceae (1/1)
• Chenopodiaceae (3/5)
• Compositae (2/2)
• Cucurbitaceae (1/1)
• Gramineae (1/1)
• Leguminosae-Papilionoideae (2/6)
• Malvaceae (1/1)
• Solanaceae (6/7)
• Tetragoniaceae (1 /1)
• Umbelliferae (1/1)

Sources of host-range data

Tsuchizaki et al. (1971); Tsuchizaki (1975).

Physical and biochemical properties

Properties of particles in sap

TIP: 50-60 °C (in Glycine max sap). LIV: 3-5 days (at room temperature). DEP: log₁₀ minus 3-4. Leaf sap contains few virions.

Purification method

Tsuchizaki et al. (1971).

Particle morphology

Virions isometric; not enveloped; 22-25 nm in diameter; angular in profile; with a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 122 S (B₁ and B₂); of the other(s) 93 S (M), or 50 S (T). Density 1.504 g cm^-3 in CsCl (B₂), or 1.497 g cm^-3 in CsCl (B₁), or 1.413 g cm^-3 in CsCl (T).

Biochemical properties

Virions contain 41 % nucleic acid (B₂), or 40.1 % nucleic acid (B₁), or 29.5 % nucleic acid (M), or 0 % nucleic acid (T); 59 % protein (B₂), or 59.9 % protein (B₁), or 70.5 % protein (M), or 100 % protein (T).

Genome consists of RNA; single-stranded; linear. Total genome size 11.2 kb. Genome of two parts; largest (or only) genome part the larger 7.3 kb; the 2nd largest 3.9 kb. Genomic nucleic acid isolated by Hibi et al. (1984). Infectivity lost when deproteinised with phenol or detergent.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in mesophyll and epidermis; in cytoplasm. Inclusions present in infected cells; are membranous bodies (with vesicles). Other cellular changes: tubules containing a single row of virions in the cytoplasm.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, cherry leaf roll, grapevine chrome mosaic, grapevine fanleaf, raspberry ringspot, strawberry latent ringspot, tobacco ringspot, tomato black ring and tomato ringspot viruses.

Comments and References

References

• Hibi, T., Yagita, H. and Iwaki, M. (1984). Ann. Phytopath. Soc. Japan 50: 445.
• Tsuchizaki, T. (1975). CMI/AAB Descr. Pl. Viruses No. 142, 3 pp.
• Tsuchizaki, T., Hibino, H. and Saito, Y. (1971). Ann. Phytopath. Soc. Japan 37: 266.
• Yagita, H. and Komuro, Y. (1972). Ann. Phytopath. Soc. Japan 38: 275.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.