Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Melon necrotic spot carmovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by T. Hibi, 1986.

Nomenclature

Acronym

MNSV

Strains

Japanese isolate, Californian isolate, European isolate.

ICTV decimal code

74.0.2.0.009

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Cucumis melo - necrotic spotting.
• Cucumis sativus - chlorotic spotting.

Transmission

Transmitted by a vector; a fungus; Olpidium radicale; Chytridiales. Virus transmitted by mechanical inoculation; transmitted by contact between plants; transmitted by seed (10-40% in melon).

Geographical distribution

Spreads in Japan, the Netherlands, the UK, and the USA.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Cucumis melo - necrotic local lesions then, occasionally, systemic necrosis.
• Cucumis sativus - chlorotic local lesions then, occasionally, systemic chlorosis or spotting.
• Citrullus lanatus - local lesions, not systemic.
• Vigna unguiculata ssp. sesquipedalis - necrotic spots; not systemic.
• Cucurbita moschata, Lagenaria siceraria - local lesions; not systemic.

Diagnostically insusceptible host species

Chenopodium amaranticolor, Chenopodium quinoa, Nicotiana glutinosa.

Maintenance and propagation hosts

Cucumis melo.

Assay hosts (Local lesions or Whole plants)

Cucumis melo (L) and (W) Cucumis sativus (L).

Susceptible host species

• Citrullus lanatus
• Cucumis melo
• Cucumis sativus
• Cucurbita moschata
• Lagenaria siceraria
• Vigna unguiculata
• Vigna unguiculata ssp. sesquipedalis

Insusceptible host species

• Allium cepa
• Apium graveolens
• Beta vulgaris
• Brassica campestris ssp. rapa
• Brassica oleracea var. capitata
• Capsicum annuum
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucurbita maxima
• Cucurbita pepo
• Glycine max
• Gomphrena globosa
• Lactuca sativa
• Nicotiana glutinosa
• Phaseolus vulgaris
• Pisum sativum
• Spinacia oleracea
• Trifolium repens
• Vicia faba

Families containing susceptible hosts

• Cucurbitaceae (5/7)
• Leguminosae-Papilionoideae (2/7)

Families containing insusceptible hosts

• Alliaceae (1/1)
• Amaranthaceae (1/1)
• Chenopodiaceae (4/4)
• Compositae (1/1)
• Cruciferae (2/2)
• Cucurbitaceae (2/7)
• Leguminosae-Papilionoideae (5/7)
• Solanaceae (2/2)
• Umbelliferae (1/1)

Sources of host-range data

Hibi and Furuki (1985); Furuki (1981).

Physical and biochemical properties

Properties of particles in sap

TIP: 60 °C. LIV: 9-32 days. DEP: log₁₀ minus 4-5. Leaf sap contains few virions.

Purification method

Hibi and Furuki (1985).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; angular in profile; with a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 134 S. Density 1.33-1.34 g cm^-3 in CsCl (unfixed).

Biochemical properties

Virions contain 17.8 % nucleic acid; 82.2 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 4.266 kb. Genome unipartite; largest (or only) genome part 4.3 kb. Genomic nucleic acid isolated by Hibi et al. (1980).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00562 Em(40)_vi:MNSCP Gb(84)_vi:MNSCP Melon necrotic spot virus coat protein gene, complete cds. 3/92 1,173bp.
• D12536 Em(40)_vi:MNSCG1 Gb(84)_vi:D12536 Melon necrotic spot virus genomic RNA, almost complete sequence. 10/92 4,262bp.
• M29671 Em(40)_vi:MNSCG Gb(84)_vi:MNSCG Melon necrotic spot virus, complete genome. 10/92 4,266bp.
• D29662 Em(43)_vi:Mnscp1 Gb(89)_vi:Mnscp1 Melon necrotic spot virus (strain NH) gene for coat protein. 4/95 1,173bp.
• D29663 Em(43)_vi:Mnscp2 Gb(89)_vi:Mnscp2 Melon necrotic spot virus (strain S) gene for coat protein. 4/95 1,173bp.

Features of proteins

M_r 46000; coat protein. Method of preparation: Bos et al. (1984).

Replication

Genome replicates in cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, roots, stems, petioles, fruits and seeds; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are crystals in the cytoplasm; they contain virions.

Taxonomy and relationships

Carmovirus: Tombusviridae

Virus(es) with serologically unrelated virions

Cucumber necrosis, wild cucumber mosaic, carnation mottle, tobacco necrosis, turnip yellow mosaic, carnation ringspot, cucumber mosaic, pelargonium leaf curl, squash mosaic, cucumber soil-borne, cucumber leaf spot and tomato bushy stunt viruses.

Comments and References

References

• Bos, L., van Dorst, H.J.M., Huttinga, H. and Maat, D.Z. (1984). Neth. J. Pl. Path. 90: 55.
• Furuki, I. (1981). Tech. Bull. Shizuoka Agric. Exp. Stn 14: 94.
• Hibi, T., Furuki, I., Honda, Y., Saito, Y. and Komuro, Y. (1980). Ann. Phytopath. Soc. Japan 46: 419.
• Hibi, T. and Furuki, I. (1985). CMI/AAB Descr. Pl. Viruses No. 302, 4 pp.
• Kishi, K. (1966). Ann. Phytopath. Soc. Japan 32: 138.
• Riviere, C.J. and Rochon, D.M. (1990). J. gen. Virol.69: 395.
• Tomlinson, J.A. and Thomas, B.J. (1986). Ann. appl. Biol. 108: 71.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.