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Descriptions and Lists from the VIDE Database

Lucerne transient streak sobemovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.L.S. Forster, 1982. Revised 1983.

Nomenclature

Acronym

LTSV

ICTV decimal code

67.0.1.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms disappear soon after infection.
• Medicago sativa - vein yellowing.

Transmission

Virus transmitted by mechanical inoculation; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Australia, Canada, and New Zealand.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - necrotic local lesions; systemic chlorotic blotching (Australian, but not Ne Zealand isolates).
• Chenopodium quinoa - necrotic and chlorotic local lesions; systemic mosaic.
• Medicago scutellata - necrotic local lesions.
• Pisum sativum - necrotic local lesions (New Zealand isolates), or symptomless (Australian isolates); no systemic infection.
• Nicotiana clevelandii - chlorotic local lesions; systemic mosaic.

Diagnostically insusceptible host species

Cucumis sativus, Gomphrena globosa, Phaseolus vulgaris, Nicotiana glutinosa, Vigna unguiculata.

Maintenance and propagation hosts

Chenopodium quinoa, Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L), Pisum sativum (L).

Susceptible host species

• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cicer arietinum
• Lupinus albus
• Lupinus angustifolius
• Medicago sativa
• Medicago scutellata
• Nicotiana clevelandii
• Nicotiana rustica
• Nicotiana tabacum
• Petunia × hybrida
• Pisum sativum
• Spinacia oleracea
• Trifolium incarnatum
• Vicia faba
• Vicia sativa
• Zinnia elegans

Insusceptible host species

• Arachis hypogaea
• Brassica campestris ssp. pekinensis
• Calendula officinalis
• Capsicum annuum
• Capsicum frutescens
• Crotalaria spectabilis
• Cucumis sativus
• Cucurbita maxima
• Datura stramonium
• Dianthus barbatus
• Glycine max
• Gomphrena globosa
• Lablab purpureus
• Lactuca sativa
• Lotus corniculatus
• Lycopersicon esculentum
• Macroptilium lathyroides
• Macrotyloma uniflorum
• Nicotiana glutinosa
• Nicotiana sylvestris
• Phaseolus lunatus
• Phaseolus vulgaris
• Physalis floridana
• Solanum melongena
• Solanum nigrum
• Trifolium pratense
• Trifolium repens
• Trifolium subterraneum
• Vicia faba
• Vigna angularis
• Vigna unguiculata
• Vigna unguiculata ssp. cylindrica

Families containing susceptible hosts

• Chenopodiaceae (4/4)
• Compositae (1/3)
• Leguminosae-Papilionoideae (9/24)
• Solanaceae (4/13)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Caryophyllaceae (1/1)
• Compositae (2/3)
• Cruciferae (1/1)
• Cucurbitaceae (2/2)
• Leguminosae-Papilionoideae (16/24)
• Solanaceae (9/13)

Sources of host-range data

Blackstock (1978); Forster and Jones (1979).

Physical and biochemical properties

Properties of particles in sap

TIP: 70 °C. LIV: 28 days. DEP: log₁₀ minus 5. Leaf sap contains few virions, or contains many virions.

Purification method

Forster and Jones (1979).

Particle morphology

Virions isometric; not enveloped; 27-28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement (ring-like subunits on some virions after freeze-dehydration and staining).

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 112 S. Density 1.37 g cm^-3 in CsCl.

Biochemical properties

Virions contain 18 % nucleic acid; 82 % protein; 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 4.23 kb. Genome unipartite; largest (or only) genome part 4.23 kb. 5´ terminus of RNA has a VPg. Infectivity lost when deproteinised with proteases; retained when deproteinised with phenol or detergent. Poly A region absent.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• D00341 Em(40)_vi:MTSCA Gb(84)_vi:MTSCA Lucerne transient streak virus(LTSV-C) RNA2, complete. 4/90 322bp.
• X01984 Em(40)_vi:LTSVARN2 Gb(84)_vi:LTSVARN2 Lucerne transient streak virus (LTSV-A) RNA 2 sequence. 7/93 324bp.
• X01985 Em(40)_vi:LTSVNRN2 Gb(84)_vi:LTSVNRN2 Lucerne transient streak virus (LTSV-N) RNA2 sequence. 7/93 324bp. 3 sequences.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA and satellite RNA. Sub-genomic mRNA found in infected cells; codes for the coat protein and has M_r 35000.

Features of proteins

Virion protein(s) one (with a minor component that is probably a hydrolysis product of it); M_r 32000; coat protein. M_r of 2nd largest 29000. Method of preparation: Forster and Jones (1979).

Virus-coded non-virion proteins identified by genomic sequence analysis; three proteins found. M_r of the largest 105000. M_r of 2nd largest 78000. M_r of 3rd 18000.

Replication

Replication does not depend on a helper virus. Acts as helper for a satellite RNA.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm and in cell vacuoles. Inclusions absent from infected cells.

Taxonomy and relationships

Sobemovirus

Virus(es) with serologically related virions

Subterranean clover mottle virus, but distantly.

Comments and References

References

• Blackstock, J.M. (1974). Aust. Pl. Path. Soc. Newsl. 3: 6.
• Blackstock, J.M. (1978). Aust. J. agric. Res. 29: 291.
• Forster, R.L.S. and Jones, A.T. (1979). Ann. appl. Biol. 93: 181.
• Forster, R.L.S. and Jones, A.T. (1980). CMI/AAB Descr. Pl. Viruses No. 224, 4 pp.
• Jones, A.T., Mayo, M.A. and Duncan G.H. (1983). J. gen. Virol. 64: 1167.
• Morris-Krsinich, B.A.M., Forster, R.L.S. and Mossop, D.W. (1983). Virology 124: 349.
• Tien, P., Davies, C., Hatta, T. and Francki, R.I.B. (1981). FEBS Lett. 132: 353.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.