Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Lucerne Australian symptomless (?) nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.T. Jones, 1987.

Nomenclature

Synonyms

lucerne Australian latent virus SM strain.

Acronym

LASV

ICTV decimal code

18.0.3.T.004

Host range and symptoms

Natural host range and symptoms

Symptoms none (but infection persists).
• Medicago sativa - symptomless.

Transmission

Virus transmitted by mechanical inoculation; transmitted by seed (up to 6% in Chenopodium quinoa).

Geographical distribution

Spreads in Australia.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - faint systemic mottle.
• C. murale - chlorotic or necrotic local lesions; then systemic necrotic spots and epinasty of apical leaves.
• C. quinoa - faint systemic mottle.
• Gomphrena globosa - red rings spots; not systemic.
• Nicotiana benthamiana, N. clevelandii, N. tabacum cvs White Burley, Samsun NN, Pisum sativum, Spinacia oleracea - symptomless.
• Nicotiana debneyi and Petunia × hybrida - local symptomless infection.

Diagnostically insusceptible host species

Brassica campestris ssp. rapa, B. nigra, Catharanthus roseus, Cucumis sativus, Datura stramonium, Lycopersicon esculentum, Nicotiana glutinosa, N. rustica, Phaseolus vulgaris, Tetragonia tetragonioides, Vicia faba.

Maintenance and propagation hosts

Chenopodium quinoa.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L); C. murale (L).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Gomphrena globosa
• Medicago sativa
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana tabacum
• Petunia × hybrida
• Pisum sativum
• Spinacia oleracea

Insusceptible host species

• Brassica campestris ssp. rapa
• Brassica nigra
• Catharanthus roseus
• Cucumis sativus
• Datura stramonium
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana rustica
• Phaseolus vulgaris
• Tetragonia tetragonioides
• Vicia faba

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (4/4)
• Leguminosae-Papilionoideae (2/4)
• Solanaceae (5/9)

Families containing insusceptible hosts

• Apocynaceae (1/1)
• Cruciferae (2/2)
• Cucurbitaceae (1/1)
• Leguminosae-Papilionoideae (2/4)
• Solanaceae (4/9)
• Tetragoniaceae (1/1)

Physical and biochemical properties

Properties of particles in sap

TIP: 50-55 °C. LIV: greater than 24 days. DEP: log₁₀ minus 2-3. Leaf sap contains few virions. Electron microscopy: most virions are either penetrated by stain or disrupted, when stained with 2% AM, MT or UA.

Purification method

Remah et al. (1986).

Particle morphology

Virions isometric; not enveloped; 26-28 nm in diameter; angular in profile.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 130 S.

Biochemical properties

Genome consists of RNA; single-stranded. Total genome size 10.9 kb. Genome of two parts (plus 2 minor uncharacterized components of lengths 0.85 kb and 0.56 kb); largest (or only) genome part the larger 7 kb; the 2nd largest 3.9 kb. Genomic nucleic acid isolated by Remah et al. (1986). Infectivity lost when deproteinised with proteases.

Features of proteins

Virion protein(s) two; M_r of the larger 40000. M_r of 2nd largest 26000. Method of preparation: Remah et al. (1986).

Replication

Replication does not depend on a helper virus.

Taxonomy and relationships

Possible Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arracacha B (strains O and T), bean pod mottle, broad bean mottle, broad bean true mosaic, broad bean stain, broad bean wilt (serotype 1 and 2), cowpea mosaic, crimson clover latent, cucumber mosaic, lucerne transient streak, rubus Chinese seed-borne, strawberry latent ringspot and tobacco ringspot viruses.

Additional comments on relationships

The virus is similar to strawberry latent ringspot (Murant, 1974) and its relative rubus Chinese seed-borne virus (Barbara et al., 1985), which are tentative members of the nepovirus group (Harrison and Murant, 1977; Barbara et al., 1985).

Comments and References

References

• Barbara, D.J., Ashby, S.C. and McNamara, D.G. (1985). Ann. appl. Biol. 107: 45.
• Blackstock, J.M. (1978). Aust. J. agric. Res. 29: 291.
• Harrison, B.D. and Murant, A.F. (1977). CMI/AAB Descr. Pl. Viruses No. 185, 4 pp.
• Murant, A.F. (1974). CMI/AAB Descr. Pl. Viruses No. 126, 4 pp.
• Remah, A., Jones, A.T. and Mitchell, M.J. (1986). Ann. appl. Biol. 109: 307.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.