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Descriptions and Lists from the VIDE Database

Lilac ring mottle ilarvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by F.A. van der Meer, 1987.

Nomenclature

Acronym

LRMV

ICTV decimal code

10.0.2.07.01

Host range and symptoms

Natural host range and symptoms

Symptoms vary cyclically over a few weeks and vary seasonally (infected plants show symptoms in spring; cvs Mme Florent Stepman and Mme Lemoine show clear symptoms).
• Syringa vulgaris - faint rings and line patterns.

Transmission

Virus transmitted by mechanical inoculation; transmitted by grafting; transmitted by contact between plants; transmitted by seed (in Chenopodium quinoa, C. amaranticolor, but not in lilac (Van der Meer, 1976)).

Ecology and control

Studies reported by Van der Meer (1976); Van der Meer et al. (1976).

Geographical distribution

Found, but with no evidence of spread, in Canada, the Netherlands and the U.K.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa - systemic epinasty and tip necrosis.
• Celosia plumosa - chlorotic flecks, then systemic chlorosis.
• Nicotiana megalosiphon - necrotic local lesions, then systemic mottling.
• Nicotiana tabacum - transient wilting of the first leaves to be systemically infected.

Diagnostically insusceptible host species

Cucumis sativus, Amaranthus caudatus, Capsicum annuum, Lycopersicon esculentum.

Maintenance and propagation hosts

Chenopodium quinoa.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (W).

Susceptible host species

• Catharanthus roseus
• Celosia argentea
• Celosia plumosa
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium foetidum
• Chenopodium quinoa
• Gomphrena globosa
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana tabacum
• Phaseolus vulgaris
• Syringa vulgaris
• Vigna unguiculata

Insusceptible host species

• Amaranthus caudatus
• Capsicum annuum
• Cucumis sativus
• Lycopersicon esculentum

Families containing susceptible hosts

• Amaranthaceae (3/4)
• Apocynaceae (1/1)
• Chenopodiaceae (4/4)
• Leguminosae-Papilionoideae (2/2)
• Oleaceae (1/1)
• Solanaceae (5/7)

Families containing insusceptible hosts

• Amaranthaceae (1/4)
• Cucurbitaceae (1/1)
• Solanaceae (2/7)

Sources of host-range data

Van der Meer (1976).

Physical and biochemical properties

Properties of particles in sap

TIP: 63-65 °C. LIV: 0.2 days (at 20ºC). DEP: log₁₀ minus 4 (in sap of Chenopodium quinoa). Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains few virions. Electron microscopy: fix virions with 2% glutaraldehyde.

Purification method

Van der Meer et al. (1976).

Particle morphology

Virions isometric; not enveloped; 27 nm in diameter; rounded in profile; without a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest (B) component 98 S; of the other(s) 83 S (M). Density 1.345 g cm^-3 in CsCl. Density 1.265 in Cs₂SO₄. A₂₆₀/A₂₈₀ ratio 1.43-1.46.

Biochemical properties

Virions contain 13-14 % nucleic acid; 86-87 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 9.042 kb. Genome of three parts; largest (or only) genome part the largest 3.324 kb; the 2nd largest 3.183 kb; the 3rd largest 2.535 kb. Genomic nucleic acid isolated by Huttinga and Mosch (1976). Infectivity retained when deproteinised with phenol or detergent. Additional factor required for infectivity; is coat protein plus RNA 1, 2 and 3.

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• U17391 Em(43)_vi:Lr17391 Gb(89)_vi:Lru17391 Lilac ring mottle virus RNA 3 movement protein and coat protein genes, complete cds. 5/95.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA (RNA-4 1.042 kb). Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 27500; coat protein. Method of preparation: Huttinga and Mosch (1976).

Replication

Replication does not depend on a helper virus.

Taxonomy and relationships

Ilarvirus: Bromoviridae (subgroup 7)

Virus(es) with serologically unrelated virions

Apple mosaic, elm mottle, prunus necrotic ringspot, spinach latent and tobacco streak viruses.

Additional comments on relationships

Serological tests quickly distinguish lilac ring mottle virus from related viruses.

Best tests for diagnosis

Ten other sap transmissible viruses are known to infect lilac (Van der Meer, 1980, 1981; Waterworth, 1972), thus identification of lilac ring mottle virus by testing in standard indicator plants, like Chenopodium and Nicotiana species, is unreliable and serological tests are required.

Comments and References

References

• Bos, L., Huttinga, H. and Maat, D.Z. (1980). Neth. J. Pl. Path. 86: 79.
• Fulton, R.W. (1983). CMI/AAB Descr. Pl. Viruses No. 275, 3 pp.
• Huttinga, H. and Mosch, W.H.M. (1976). Acta Hort. 59: 113.
• Huttinga, H., Maat, D.Z. and van der Meer, F.A. (1976). Acta Bot. neerl. 25: 251.
• Van der Meer, F.A. (1976). Acta Hort. 59: 105.
• Van der Meer, F.A. (1980). Acta Hort. 110: 211.
• Van der Meer, F.A. and Huttinga, H. (1979). CMI/AAB Descr. Pl. Viruses No. 201, 4 pp.
• Van der Meer, F.A., Huttinga, H. and Maat, D.Z. (1976). Neth. J. Pl. Path. 82: 67.
• Waterworth, H.E. (1972). Pl. Dis. Reptr 56: 923.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.