Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Lato River tombusvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by C. Vovlas, 1991.

Nomenclature

Acronym

LRV

ICTV decimal code

74.0.1.0.009

Host range and symptoms

Transmission

Most probably spread in irrigation water. Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in Italy.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show local lesions, systemic mosaic, and death.

Diagnostically susceptible host species and symptoms

• Chenopodium quinoa, Gomphrena globosa - local lesions.
• Nicotiana benthamiana, N. clevelandii - systemic mosaic.

Diagnostically insusceptible host species

Nicotiana glutinosa, Chenopodium amaranticolor.

Maintenance and propagation hosts

Nicotiana benthamiana, N. clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium quinoa (L), Gomphrena globosa (L), Nicotiana benthamiana (W), N. clevelandii (W).

Susceptible host species

• Chenopodium quinoa
• Gomphrena globosa
• Nicotiana benthamiana
• Nicotiana clevelandii

Insusceptible host species

• Chenopodium amaranticolor
• Nicotiana glutinosa

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (1/2)
• Solanaceae (2/3)

Families containing insusceptible hosts

• Chenopodiaceae (1/2)
• Solanaceae (1/3)

Purification method

Blend infected leaves of Nicotiana benthamiana in phosphate buffer (pH 7.2). Clarify with magnesium bentonite.

Physical and biochemical properties

Particle morphology

Virions isometric; 30 nm in diameter (also some 17 nm in diameter); rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations. Density 1.28 g cm^-3 in CsCl, or 1.33 g cm^-3 in CsCl.

Biochemical properties

Virions contain 18 % nucleic acid; 82 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 4.7 kb. Genome unipartite; largest (or only) genome part 4.7 kb. Genomic nucleic acid isolated by Vovlas: phenol-SDS. Infectivity retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA.

Features of proteins

Virion protein(s) one (plus a fragment); M_r 38000 (plus 29000); coat protein. Method of preparation: Laemmli (1970).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves and all parts of the host plant; in cytoplasm and in cell vacuoles.

Taxonomy and relationships

Tombusvirus: Tombusviridae

Virus(es) with serologically related virions

Moroccan pepper, artichoke mottled crinkle and Neckar River tombusviruses.

Virus(es) with serologically unrelated virions

Cymbidium ringspot and grapevine Algerian latent viruses.

Comments and References

General comments

Purified preparations, after cool storage, contain 30 nm diameter virions and also some about 17 nm in diameter that contain no RNA. The small virions are formed of the coat protein of the larger; such virions are not produced by other tombusviruses.

References

• Gallitelli, D., Di Franco, A., Vovlas, C. and Martelli, G.P. (1989). J. Phytopath. 126: 213.
• Laemmli, U.K. (1970). Nature, Lond. 227: 680.
• Vovlas, C. (1986). Proc. 8th Wksh. Epidem. Pl. Virus Dis., Orlando, Florida, U.S.A. p. 13.
• Vovlas, C. and Di Franco, A. (1987). Inform. Fitopatol. 3: 55.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.