Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Kyuri green mottle mosaic tobamovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by R.I.B. Francki, 1988.

Nomenclature

Synonyms

Japanese CV3, cucumber green mottle mosaic virus - cucumber strain or strain C (Francki et al., 1986).

Acronym

KGMMV

Strains

C and Y isolates or strains.

ICTV decimal code

71.0.1.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Cucumis sativus - mosaic, severe fruit mottling and malformation.

Transmission

Transmitted by means not involving a vector. Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in Japan.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Cucumis sativus - mottling, and fruit malformation.
• Datura stramonium, Petunia × hybrida - diffuse chlorotic local lesions; not systemic.

Maintenance and propagation hosts

Cucumis sativus.

Assay hosts (Local lesions or Whole plants)

Datura stramonium (L), Cucumis sativus (W).

Susceptible host species

• Cucumis melo
• Cucumis sativus
• Cucurbita maxima
• Cucurbita moschata
• Cucurbita pepo
• Datura stramonium
• Nicotiana tabacum
• Petunia × hybrida

Insusceptible host species

• Beta vulgaris
• Catharanthus roseus
• Chenopodium amaranticolor
• Glycine max
• Gomphrena globosa
• Lactuca sativa
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana rustica
• Phaseolus vulgaris
• Raphanus sativus
• Spinacia oleracea
• Tetragonia tetragonioides
• Vicia faba
• Zinnia elegans

Families containing susceptible hosts

• Cucurbitaceae (5/5)
• Solanaceae (3/6)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Apocynaceae (1/1)
• Chenopodiaceae (3/3)
• Compositae (2/2)
• Cruciferae (1/1)
• Leguminosae-Papilionoideae (3/3)
• Solanaceae (3/6)
• Tetragoniaceae (1/1)

Sources of host-range data

Inouye et al. (1967b).

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains many virions. Electron microscopy: no special treatments needed.

Purification method

Tung and Knight (1972); Francki and McLean (1968).

Particle morphology

Virions rod-shaped; not enveloped; usually straight; with a clear modal length; of c. 300 nm; c. 15 nm wide. Axial canal obvious. Basic helix obvious.

Biochemical properties

Virions contain c. 5 % nucleic acid; c. 95 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 6.5 kb. Genome unipartite; largest (or only) genome part 6.5 kb. Genomic nucleic acid isolated by Peden and Symons (1973). Infectivity retained when deproteinised with phenol or detergent. Additional factor not required for infectivity.

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA. Sub-genomic mRNA found in infected cells.

Features of proteins

Virion protein(s) one; M_r 17000; coat protein. Method of preparation: Fraenkel-Conrat (1957); Tung and Knight (1972). Amino acid sequence: Nozu et al. (1971); Tung and Knight (1972); Kurachi et al. (1972).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in leaves, mesophyll, epidermis, vascular parenchyma, xylem, phloem, companion cells and all parts of the host plant; in cytoplasm and in cell vacuoles. Inclusions present in infected cells; are crystals in the cytoplasm; they contain virions. Other cellular changes: vesiculation of mitochondria.

Taxonomy and relationships

Tobamovirus

Virus(es) with serologically related virions

Cucumber green mottle mosaic (SDI:6), ribgrass mosaic (SDI:5), frangipani mosaic (SDI:5), odontoglossum ringspot (SDI:4), tobacco mosaic (SDI:7) and tobacco mild green mosaic (SDI:6) viruses.

Virus(es) with serologically unrelated virions

Sunn-hemp mosaic.

Additional comments on relationships

Biologically kyuri green mottle mosaic and cucumber green mottle mosaic viruses are similar but have little genomic sequence homology and are serologically distantly related.

Comments and References

General comments

Although long considered to be a strain of cucumber green mottle mosaic tobamovirus, this virus has been shown to be a distinct tobamovirus (Francki et al., 1986).

References

• Fraenkel-Conrat, H. (1957). Virology 4: 1.
• Francki, R.I.B. and McLean, G.D. (1968). Aust. J. biol. Sci. 21: 1311.
• Francki, R.I.B., Hu, J. and Palukaitis, P. (1986). Intervirology 26: 156.
• Inouye, T., Inouye, N., Asatani, M. and Mitsuhata, K. (1967a). Nogaku Kenkyu 51: 175.
• Inouye, T., Inouye, N., Asatani, M. and Mitsuhata, K. (1967b). Ber. Ohara Inst. landw. Biol. 14: 49.
• Kurachi, K., Funatsu, G., Funatsu, M. and Hidaka, S. (1972). Agric. Biol. Chem. 36: 1109.
• Nozu, Y., Tochihara, H., Komuro, Y. and Okada, Y. (1971). Virology 45: 577.
• Okada, Y. (1986). In: The Plant Viruses. Vol. 2, The Rod-Shaped Plant Viruses, p. 267; eds M.H.V. van Regenmortel and H. Fraenkel-Conrat. Plenum Press, New York.
• Peden, K.W.C. and Symons, R.H. (1973). Virology 53: 487.
• Tung, J.S. and Knight, C.A. (1972). Virology 48: 574.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.