Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Kalanchoe isometric virus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by M.L. Izaguirre-Mayoral, 1991.

Nomenclature

Synonyms

IVLP.

Strains

Venezuelan.

ICTV decimal code

79.0.P.DE.21

Host range and symptoms

Natural host range and symptoms

Symptoms vary seasonally. Symptoms sunken tissues and necrosis.
• Kalanchoe pinnata, K. tubiflora, K. daigremontiana - sunken tissues between leaf veins, sometimes necrosis.

Transmission

Virus transmitted by mechanical inoculation.

Geographical distribution

Spreads in Venezuela.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorotic and necrotic local lesions, chlorotic rings.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor - chlorotic local lesions.
• Chenopodium quinoa, Cucumis sativus cv. Palomar - necrotic local lesions.
• Datura stramonium - chlorotic local lesions, chlorotic rings.
• Nicotiana benthamiana - systemic mosaic.
• N. tabacum cvs Samsun, White Burley - chlorotic rings and systemic mosaic.
• Phaseolus vulgaris cv. Top crop - systemic mosaic.
• P. vulgaris cv. Red Kidney - chlorotic local lesions and systemic mosaic.
• Tetragonia tetragonioides - chlorotic rings.

Diagnostically insusceptible host species

Cucumis melo, C. sativus cv. Ashley, Cucurbita maxima, Lycopersicon esculentum, Vigna unguiculata.

Maintenance and propagation hosts

Nicotiana benthamiana.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. quinoa (L), Cucumis sativus cv. Palomar (L).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium quinoa
• Cucumis sativus (cv. Palomar)
• Datura stramonium
• Kalanchoe daigremontiana
• Kalanchoe pinnata
• Kalanchoe tubiflora
• Nicotiana benthamiana
• Nicotiana tabacum (cvs Samsun, White Burley)
• Phaseolus vulgaris
• Tetragonia tetragonioides

Insusceptible host species

• Cucumis melo
• Cucumis sativus (cv. Ashley)
• Cucurbita maxima
• Lycopersicon esculentum
• Nicotiana glutinosa
• Vigna unguiculata
• Zea mays

Families containing susceptible hosts

• Chenopodiaceae (2/2)
• Crassulaceae (3/3)
• Cucurbitaceae (1/3)
• Leguminosae-Papilionoideae (1/2)
• Solanaceae (3/5)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Cucurbitaceae (3/3)
• Gramineae (1/1)
• Leguminosae-Papilionoideae (1/2)
• Solanaceae (2/5)

Sources of host-range data

Izaguirre-Mayoral et al. (1990).

Physical and biochemical properties

Properties of particles in sap

TIP: 50 °C. LIV: 0.2 days (20 hours). Leaf sap contains few virions.

Purification method

Izaguirre-Mayoral et al. (1990).

Particle morphology

Virions isometric; not enveloped; 35 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

One sedimenting component in purified preparations. Density 1.32 g cm^-3 in CsCl. A₂₆₀/A₂₈₀ ratio 1.56.

Biochemical properties

Virions contain 9-10 % nucleic acid.

Genome consists of DNA; single-stranded. Total genome size 3.5 kb. Genome unipartite; largest (or only) genome part 3.5 kb.

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 46000.

Cytopathology

Virions found in leaves. Inclusions absent from infected cells. Other cellular changes: severely damaged chloroplasts of different forms, randomly distributed in the cytoplasm and bigger than those of non-infected leaves. Starch grains occupied 78% of the chloroplast internal volume during the night as well as in the light period, and the number of thylakoids per grana reduced to 5»2.

Taxonomy and relationships

(ungrouped)

Comments and References

References

• Izaguirre-Mayoral, M.L., Carballo, O. and Gil, F. (1990). J. Phytopath. 130: 303.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.