Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Hyacinth mosaic potyvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, 1985.

Nomenclature

Acronym

HyaMV.

ICTV decimal code

57.0.1.T.044

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Hyacinthus orientalis - leaf chlorosis, stem spotting, occasional flower break.

Transmission

Transmitted by a vector; an insect; Myzus persicae, Macrosiphum solanifolii; Aphididae. Transmitted in a non-persistent manner. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Australia, Bulgaria, the Netherlands, the UK, and the USA.

Experimental host range

Several (3-9) families susceptible.

Diagnostically insusceptible host species

Chenopodium quinoa, Lilium formosanum, Nicotiana clevelandii, Phaseolus vulgaris, Tetragonia tetragonioides.

Maintenance and propagation hosts

Hyacinthus orientalis.

Susceptible host species

• Hyacinthus orientalis

Insusceptible host species

• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Gomphrena globosa
• Lilium formosanum
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Tetragonia tetragonioides
• Trifolium incarnatum
• Vicia faba

Families containing susceptible hosts

• Hyacinthaceae (1/1)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (3/3)
• Leguminosae-Papilionoideae (3/3)
• Liliaceae (1/1)
• Solanaceae (4/4)
• Tetragoniaceae (1/1)

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Phillips: extract from infected hyacinth leaves and flower stems by blending in 0.4 M Na citrate + 0.2 M urea + 10 mM DIECA + 0.2% (w/v) hemicellulase + 0.1% (v/v) mercapto-acetic acid (pH 6.9) (1 g : 4 ml). Incubate at 37ºC for 1 hour. Add Triton X-100 (2% v/v of extractant) and stir for 30 minutes. Leave at 2ºC overnight. Filter through cheesecloth and centrifuge (20 minutes at 12,000 g; 75 minutes at 65,000 g). Resuspend pellets in 0.2 M Na-citrate + 0.1 M urea + 0.1% (v/v) mercapto-acetic acid (pH 6.6, 1 ml/1 g of plant material). Leave at 2ºC overnight. Give a second cycle of differential centrifugation and resuspend pellets in a 33 mM phosphate buffer (pH 7.3).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 740 nm. Axial canal obscure. Basic helix obscure.

Taxonomy and relationships

Potyvirus: Potyviridae

Virus(es) with serologically related virions

Ornithogalum mosaic, scilla mosaic, freesia mosaic and iris severe mosaic viruses.

Virus(es) with serologically unrelated virions

Bean yellow mosaic, hippeastrum mosaic, iris mild mosaic, narcissus yellow stripe, onion yellow dwarf, potato Y, tulip breaking and turnip mosaic viruses.

Comments and References

References

• Atanasoff, D. (1928). Bull. Soc. Bot. Bulgarie 2: 51.
• Derks, A.F.L.M. and Vink-van den Abeele, J.L. (1980). Acta Hort. 109: 495.
• Smith, F.F. and Brierley, P. (1944). Phytopathology 34: 497.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.