Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Hibiscus latent ringspot nepovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.A. Brunt, 1984.

Nomenclature

Acronym

HLRSV

ICTV decimal code

18.0.3.0.018

Host range and symptoms

Natural host range and symptoms

Symptoms disappear soon after infection.
• Hibiscus rosa-sinensis and H. cannabinus - leaf chlorosis but usually symptomless.

Transmission

Transmitted by a vector; a nematode (probably). Virus transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed (in limited tests with seed from Nicotiana clevelandii and Chenopodium quinoa).

Geographical distribution

Spreads in Italy and Nigeria.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Gomphrena globosa - grey necrotic local lesions; not systemic.
• Chenopodium murale - necrotic lesions, not systemic.
• C. quinoa - chlorotic local lesions; systemic chlorosis.
• Trifolium incarnatum - severe systemic chlorosis.
• Hibiscus cannabinus - systemic leaf chlorosis.
• Nicotiana clevelandii - systemic leaf chlorosis.

Diagnostically insusceptible host species

Phaseolus vulgaris, Abelmoschus esculentus (okra), Capsicum annuum, Lycopersicon esculentum, Physalis floridana.

Maintenance and propagation hosts

Hibiscus cannabinus, Nicotiana clevelandii.

Assay hosts (Local lesions or Whole plants)

Chenopodium murale (L), C. quinoa (L,W), Hibiscus cannabinus (W).

Susceptible host species

• Chenopodium amaranticolor
• Chenopodium murale
• Chenopodium quinoa
• Gomphrena globosa
• Gossypium hirsutum
• Hibiscus cannabinus
• Hibiscus rosa-sinensis
• Nicandra physalodes
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana glutinosa
• Nicotiana tabacum
• Nicotiana × edwardsonii
• Petunia × hybrida
• Tetragonia tetragonioides
• Trifolium incarnatum

Insusceptible host species

• Abelmoschus esculentus
• Adansonia digitata
• Beta vulgaris
• Brassica campestris ssp. pekinensis
• Brassica oleracea var. botrytis
• Capsicum annuum
• Capsicum frutescens
• Catharanthus roseus
• Citrullus lanatus
• Cucumis melo
• Cucurbita pepo
• Dahlia pinnata
• Glycine max
• Helianthus annuus
• Lactuca sativa
• Lycopersicon esculentum
• Nicotiana megalosiphon
• Nicotiana sylvestris
• Phaseolus vulgaris
• Physalis floridana
• Pisum sativum
• Sesamum indicum
• Solanum demissum
• Solanum melongena
• Solanum nigrum
• Theobroma cacao
• Trifolium repens
• Vicia faba
• Vigna unguiculata
• Zea mays
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (3/4)
• Leguminosae-Papilionoideae (1/7)
• Malvaceae (3/4)
• Solanaceae (7/16)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Apocynaceae (1/1)
• Bombacaceae (1/1)
• Chenopodiaceae (1/4)
• Compositae (4/4)
• Cruciferae (2/2)
• Cucurbitaceae (3/3)
• Gramineae (1/1)
• Leguminosae-Papilionoideae (6/7)
• Malvaceae (1/4)
• Pedaliaceae (1 /1)
• Solanaceae (9/16)
• Sterculiaceae (1/1)

Sources of host-range data

Brunt et al. (1980).

Physical and biochemical properties

Properties of particles in sap

TIP: 65 °C (in Nicotiana clevelandii sap). LIV: 21-28 days (in N. clevelandii sap). DEP: log₁₀ minus 4-5 (in N. clevelandii sap). Leaf sap contains few virions.

Particle morphology

Virions isometric; not enveloped; 28 nm in diameter; angular in profile; without a conspicuous capsomere arrangement.

Physical properties

Three sedimenting components in purified preparations; sedimentation coefficient of the fastest 132 S (B); of the other(s) 114 S (M), or 51 S (T). Density 1.52 g cm^-3 in CsCl (B), or 1.49 g cm^-3 in CsCl, or 1.32 g cm^-3 in CsCl (M; unfixed). A₂₆₀/A₂₈₀ ratio 1.85 (B), or 1.78 (M; corrected for light scattering).

Biochemical properties

Virions contain 42 % nucleic acid (B), or 38 % nucleic acid (M), or 0 % nucleic acid (T); 58 % protein (B), or 62 % protein (M), or 100 % protein (T); 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 13.2 kb. Genome of two parts; largest (or only) genome part the larger 7 kb; the 2nd largest 6.2 kb. Genomic nucleic acid isolated by Brunt et al. (1980).

Features of proteins

Virion protein(s) one; M_r 53600.

Taxonomy and relationships

Nepovirus: Comoviridae

Virus(es) with serologically unrelated virions

Arabis mosaic, arracacha A, artichoke Italian latent, cherry leaf roll, cherry rasp leaf, cacao necrosis, raspberry ringspot, strawberry latent ringspot, tobacco ringspot, tomato black ring and tomato ringspot viruses.

Comments and References

References

• Brunt, A.A., Barton, R.J., Phillips, S. and Lana, A.O. (1980). Ann. appl. Biol. 96: 37.
• Brunt, A.A., Barton, R.J. and Phillips, S. (1981). CMI/AAB Descr. Pl. Viruses No. 233, 3 pp.
• Lana, A.O. (1974). Pl. Dis. Reptr 58: 1040.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.