Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Festuca leaf streak cytorhabdovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by T. Lundsgaard, 1991.

Nomenclature

Acronym

FLSV

ICTV decimal code

62.0.4.0.004

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Festuca gigantea - chlorotic streaks of some veins of mature leaves.

Transmission

Not transmitted by Javesella pellucida, Laodelphax striatellus, Psammotettix alienus. Virus not transmitted by mechanical inoculation; not transmitted by seed.

Geographical distribution

Found, but with no evidence of spread, in Denmark and Germany.

Diagnostically susceptible host species and symptoms

• Vigna unguiculata ssp. unguiculata (N.B. protoplast infection).

Susceptible host species

• Festuca gigantea
• Vigna unguiculata ssp. unguiculata

Families containing susceptible hosts

• Gramineae (1/1)
• Leguminosae-Papilionoideae (1/1)

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions. Electron microscopy: virions are disrupted when extracted in 2% PTA. Virions are preserved by 2% AM extraction or aldehyde fixation prior to PTA staining.

Purification method

Lundsgaard (1987).

Particle morphology

Virions rhabdo- or bullet-shaped; enveloped; usually straight; with a clear modal length; of 286 nm; 61 nm wide. Axial canal obvious; 15 nm in diameter. Basic helix obvious; pitch of basic helix 4.7 nm.

Physical properties

One sedimenting component in purified preparations; sedimentation coefficient 704 S. Density 1.194 g cm^-3 in sucrose.

Biochemical properties

Genome probably consists of RNA; single-stranded. Total genome size 14 kb. Genome unipartite; largest (or only) genome part 14 kb. Genomic nucleic acid isolated by Lundsgaard (1987).

Features of the genome

Non-genomic nucleic acid found in the virions; is probably rRNA.

Features of proteins

Virion protein(s) three; M_r of the largest 58000; G protein. M_r of 2nd largest 49000; N protein. M_r of 3rd largest 20000; M protein. Method of preparation: Lundsgaard (1987). Virion proteins glycosylated; are it is G protein.

Cytopathology

Virions found in leaves roots, mesophyll and epidermis; in cytoplasm. Inclusions present in infected cells; are membranous bodies and viroplasms; they contain virions.

Taxonomy and relationships

Cytorhabdovirus: Rhabdoviridae

Virus(es) with serologically unrelated virions

Northern cereal mosaic, barley yellow striate mosaic, cereal chlorotic mottle, maize sterile stunt, digitaria striate and Moroccan wheat viruses (Lundsgaard, 1984; Greber, 1990).

Best tests for diagnosis

Serology.

Comments and References

References

• Greber, R.S. (1990). Ann. appl. Biol. 116: 259.
• Lundsgaard, T. (1984). Intervirology 22: 50.
• Lundsgaard, T. (1987). J. gen. Virol. 68: 931.
• Lundsgaard, T. (1991). J. Phytopath. (in press).
• Lundsgaard, T. and Albrechtsen, S.E. (1976). Phytopath. Z. 87: 12.
• Lundsgaard, T. and Albrechtsen, S.E. (1979). Phytopath. Z. 94: 112.
• Van Beek, N.A.M., Lohuis, D., Dijkstra, J. and Peters, D. (1985). J. gen. Virol. 66: 2485.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.