Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Erysimum latent tymovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A.J. Gibbs, 1991.

Nomenclature

Acronym

ErLV

ICTV decimal code

77.0.1.0.008

Host range and symptoms

Natural host range and symptoms

Symptoms persist, or none. Symptoms mosaics.
• Erysimum spp. - latent, mosaics.

Transmission

Transmitted by a vector; an insect; Phyllotreta spp.; Coleoptera. Transmitted in a semi-persistent manner. Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Germany.

Experimental host range

Many (>9) families susceptible. Experimentally infected plants mostly show local lesions, vein clearing, mosaics.

Diagnostically susceptible host species and symptoms

• Brassica campestris ssp. napus, B. campestris ssp. pekinensis - local lesions and mosaic.
• Cucumis sativus, Nicotiana glutinosa - mottle. Capsella bursa-pastoris - mosaic.
• Ocimum basilicum, Sinapis alba - local lesions, mosaic.
• Stellaria media, Lunaria annua - vein clearing and mottling.
• Erysimum spp. - latent.

Diagnostically insusceptible host species

Chenopodium quinoa, Nicotiana clevelandii, Nicotiana tabacum, Phaseolus vulgaris, Pisum sativum.

Maintenance and propagation hosts

Brassica campestris ssp. pekinensis, B. juncea, B. campestris ssp. napus, Lunaria annua.

Assay hosts (Local lesions or Whole plants)

Brassica campestris ssp. napus (L).

Susceptible host species

• Arabidopsis thaliana
• Arabis ludoviciana
• Barbarea vulgaris
• Brassica campestris ssp. chinensis
• Brassica campestris ssp. napus
• Brassica campestris ssp. pekinensis
• Brassica campestris ssp. rapa
• Brassica juncea
• Brassica oleracea var. botrytis
• Camelina sativa
• Capsella bursa-pastoris
• Catharanthus roseus
• Celosia argentea
• Cucumis sativus
• Erysimum crepidifolium
• Erysimum helveticum
• Erysimum perovskianum
• Erysimum pulchellum
• Erysimum sylvestre
• Fibigia clypata
• Lunaria annua
• Nicotiana glutinosa
• Nicotiana megalosiphon
• Ocimum basilicum
• Reseda odorata
• Sinapis alba
• Solanum integrifolium
• Stellaria media
• Trigonella caerulea
• Viola cornuta

Insusceptible host species

• Anthriscus cerefolium
• Antirrhinum majus
• Apium graveolens
• Arachis hypogaea
• Bellis perennis
• Beta vulgaris
• Brassica oleracea var. capitata
• Catalpa bignonioides
• Cheiranthus cheiri
• Chenopodium amaranticolor
• Chenopodium foetidum
• Chenopodium murale
• Chenopodium quinoa
• Delphinium hybridum
• Eucalyptus cloeziana
• Euphorbia marginata
• Fagopyrum esculentum
• Fragaria vesca
• Gossypium hirsutum
• Gypsophila elegans
• Helianthus annuus
• Ipomoea nil
• Lactuca sativa
• Lobelia erinus
• Matthiola incana
• Myosotis sylvatica
• Nicotiana clevelandii
• Nicotiana tabacum
• Papaver nudicaule
• Pastinaca sativa
• Petroselinum crispum
• Petunia × hybrida
• Phaseolus vulgaris
• Phlox drummondii
• Pisum sativum
• Plantago major
• Podolepis robusta
• Raphanus sativus
• Salvia splendens
• Sinningia speciosa
• Spinacia oleracea
• Tropaeolum majus
• Vicia faba
• Vigna unguiculata

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Apocynaceae (1/1)
• Caryophyllaceae (1/2)
• Cruciferae (19/23)
• Cucurbitaceae (1/1)
• Labiatae (1/2)
• Leguminosae-Papilionoideae (1/6)
• Resedaceae (1/1)
• Solanaceae (3 /6)
• Violaceae (1/1)

Families containing insusceptible hosts

• Bignoniaceae (1/1)
• Boraginaceae (1/1)
• Campanulaceae (1/1)
• Caryophyllaceae (1/2)
• Chenopodiaceae (6/6)
• Compositae (4/4)
• Convolvulaceae (1/1)
• Cruciferae (4/23)
• Euphorbiaceae (1 /1)
• Gesneriaceae (1/1)
• Labiatae (1/2)
• Leguminosae-Papilionoideae (5/6)
• Malvaceae (1/1)
• Myrtaceae (1/1)
• Papaveraceae (1/1)
• Plantaginaceae (1/1)
• Polemoniaceae (1/1)
• Polygonaceae (1 /1)
• Ranunculaceae (1/1)
• Rosaceae (1/1)
• Scrophulariaceae (1/1)
• Solanaceae (3/6)
• Tropaeolaceae (1/1)
• Umbelliferae (4/4)

Physical and biochemical properties

Properties of particles in sap

TIP: 76-78 °C. LIV: 21 days. DEP: log₁₀ minus 6. Infectivity of sap not changed by treatment with di-ethyl ether. Leaf sap contains many virions.

Purification method

Shukla and Gough (1980).

Particle morphology

Virions isometric; 27 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement.

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 113 S; of the other(s) 59 S. Density 1.26 g cm^-3 in CsCl, or 1.42 g cm^-3 in CsCl. Isoelectric point pH 5.4. A₂₆₀/A₂₈₀ ratio 1.76 (B).

Biochemical properties

Virions contain 32 % nucleic acid; 68 % protein.

Genome consists of RNA; single-stranded; linear. Total genome size 6.034 kb. Genome unipartite; largest (or only) genome part 6.034 kb. Base composition 15.6 % G; 23.9 % A; 34.5 % C; 26 % U. 5´ terminus of RNA has a methylated nucleotide cap. Infectivity retained when deproteinised with proteases; retained when deproteinised with phenol or detergent. Poly A region absent. Additional factor not required for infectivity. Genome has no tRNA-like activity.

NCBI sequence data (Entrez search)

Features of the genome

Non-genomic nucleic acid found in the virions; is subgenomic mRNA. Sub-genomic mRNA found in infected cells; virion proteins.

Features of proteins

Virion protein(s) one; M_r 21483; coat protein. Method of preparation: Srifah et al. (1990). Amino acid sequence: Srifah et al. (1990). Virion proteins not glycosylated. Virion proteins not phosphorylated.

Virus-coded non-virion proteins identified by genomic sequence analysis; two proteins found. M_r of the largest 48497; overlapping protein. M_r of 2nd largest 193811; replicase protein.

Replication

Genome replicates in association with chloroplasts (in vesicles in the outer membrane). Coat protein mRNA translated in the cytoplasm. Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions absent from infected cells.

Taxonomy and relationships

Tymovirus

Virus(es) with serologically related virions

Andean potato latent, ononis yellow mosaic and okra mosaic viruses.

Additional comments on relationships

Genome sequence homologous with all other tymoviruses but is the most distinct of the group.

Comments and References

References

• Gough, K., Lilley, G.G., Shukla, D.D. and Woods, E.F. (1982). Aust. J. biol. Sci. 35: 5.
• Shukla, D.D. and Gough, K. (1980). CMI/AAB Descr. Pl. Viruses No. 222, 4 pp.
• Shukla, D.D., Koenig, R., Gough, K.H., Huth, H. and Lesemann, D.-E. (1980). Phytopathology 70: 382.
• Shukla, D.D., Proll, E., Schmelzer, K. and Schmidt, H.B. (1973). Acta phytopath. Acad. Sci. Hung. 8: 91.
• Srifah, P., Keese, P., Shukla, D.D. and Gibbs, A.J. (1990). J. gen. Virol. 71: 3085.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.