Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Epirus cherry ourmiavirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by A. Avgelis, 1991.

Nomenclature

Acronym

EpCV

ICTV decimal code

79.0.P.4.DE.2

Host range and symptoms

Natural host range and symptoms

Symptoms persist. Symptoms rasp-leaf symptoms.
• Prunus avium - enations on abaxial leaf surfaces, stunting.

Transmission

Not transmitted by Myzus persicae. Virus transmitted by mechanical inoculation; transmitted by seed.

Geographical distribution

Found, but with no evidence of spread, in Epirus, Greece.

Experimental host range

Several (3-9) families susceptible. Experimentally infected plants mostly show chlorotic or necrotic local lesions or ringspots, then systemic mottle or chlorotic or necrotic ringspots.

Diagnostically susceptible host species and symptoms

• Abelmoschus esculentus - chlorotic local lesions, then systemic yellow ringspots.
• Cucumis sativus - chlorotic local lesions.
• Datura stramonium - systemic mottle and ringspotting.
• Gomphrena globosa - necrotic local lesions, then systemic necrotic ring spots and malformation.
• Nicotiana tabacum cv. Xanthi-nc - local and systemic necrotic ringspots.
• Petunia × hybrida - local and systemic chlorotic ring spots.
• Phaseolus vulgaris - reddish local lesions.
• Vicia faba - reddish local lesions, then systemic chlorotic mottle and necrotic spots.
• Vigna unguiculata - reddish local lesions, then systemic necrotic spots and tip necrosis.

Diagnostically insusceptible host species

Capsicum annuum, Cucurbita pepo, Nicotiana glutinosa, Ocimum basilicum, Zinnia elegans.

Maintenance and propagation hosts

Gomphrena globosa, Nicotiana benthamiana, Vicia faba.

Assay hosts (Local lesions or Whole plants)

Abelmoschus esculentus (W), Chenopodium amaranticolor (L), Gomphrena globosa (L and W), Phaseolus vulgaris (L), Vicia faba (W).

Susceptible host species

• Abelmoschus esculentus
• Beta vulgaris
• Celosia cristata
• Chenopodium amaranticolor
• Chenopodium quinoa
• Cicer arietinum
• Cucumis sativus
• Datura metel
• Datura stramonium
• Gomphrena globosa
• Lactuca sativa
• Luffa acutangula
• Lupinus albus
• Lycopersicon esculentum
• Momordica balsamina
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana megalosiphon
• Nicotiana rustica
• Nicotiana tabacum
• Petunia × hybrida
• Phaseolus vulgaris
• Physalis floridana
• Pisum sativum
• Prunus avium
• Spinacia oleracea
• Vicia faba
• Vigna radiata
• Vigna unguiculata

Insusceptible host species

• Allium cepa
• Brassica oleracea var. botrytis
• Brassica oleracea var. capitata
• Capsicum annuum
• Capsicum frutescens
• Citrullus lanatus
• Cucumis melo
• Cucumis metuliferus
• Cucurbita maxima
• Cucurbita pepo
• Helianthus annuus
• Lens culinaris
• Medicago sativa
• Nicotiana glutinosa
• Ocimum basilicum
• Petroselinum crispum
• Raphanus sativus
• Solanum melongena
• Solanum tuberosum
• Zea mays
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (2/2)
• Chenopodiaceae (4/4)
• Compositae (1/3)
• Cucurbitaceae (3/8)
• Leguminosae-Papilionoideae (7/9)
• Malvaceae (1/1)
• Rosaceae (1/1)
• Solanaceae (10/15)

Families containing insusceptible hosts

• Alliaceae (1/1)
• Compositae (2/3)
• Cruciferae (3/3)
• Cucurbitaceae (5/8)
• Gramineae (1/1)
• Labiatae (1/1)
• Leguminosae-Papilionoideae (2/9)
• Solanaceae (5/15)
• Umbelliferae (1 /1)

Sources of host-range data

Avgelis et al. (1989).

Physical and biochemical properties

Properties of particles in sap

TIP: 50 °C. LIV: 15 days. DEP: log₁₀ minus 5. Leaf sap contains few virions. Electron microscopy: fix with glutaraldehyde.

Purification method

Avgelis et al. (1989).

Particle morphology

Virions bacilliform; 26 nm in length, or 34 nm in length, or 43 nm in length; 18 nm wide.

Physical properties

Three sedimenting components in purified preparations. A₂₆₀/A₂₈₀ ratio 0.62-0.64 (unfractionated preparations).

Biochemical properties

Virions contain 15 % nucleic acid; 85 % protein.

Genome consists of RNA; single-stranded. Total genome size 4.5 kb. Genome of three parts; largest (or only) genome part the largest 2.45 kb; the 2nd largest 1.05 kb; the 3rd largest 1 kb. Genomic nucleic acid isolated by Boccardo et al. (1985); Barba et al. (1991).

Features of proteins

Virion protein(s) one; M_r 21400; coat protein. Method of preparation: Boccardo and Milne (1975).

Cytopathology

Virions found in the leaves.

Taxonomy and relationships

ourmiavirus

Additional comments on relationships

The size and shape of the virions of epirus cherry and melon Ourmia viruses (Lisa et al., 1988) are like those of alfalfa mosaic virus. However epirus cherry virus is not an alfamovirus but seems to be related to cassava Ivorian bacilliform virus (Aiton et al., 1988), olive latent 2, melon Ourmia and pelargonium zonate spot viruses, but no serological relationship between their virions has been shown.

Best tests for diagnosis

Electron microscopy.

Comments and References

References

• Avgelis, A., Barba, M. and Rumbos, J. (1988). Acta Hort. 235: 245.
• Avgelis, A., Barba, M. and Rumbos, J. (1989). P. Phytopath. 126: 51.
• Aiton, M.M., Lennon, A.M., Roberts, I.M. and Harrison, B.D. (1988). Abstr. 5th Int. Congr. Pl. Path. Kyoto, Japan, p. 43.
• Barba, M., Riccioni, L. and Avgelis, A. (1991). J. Phytopath. in press.
• Boccardo, G. and Milne, R.G. (1975). Virology 68: 29.
• Boccardo, G., Milne, R.G., Luisoni, E., Lisa, V. and Accotto, G.P. (1985). Virology 147: 29.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.