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Descriptions and Lists from the VIDE Database

Eggplant mosaic tymovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by C. Büchen-Osmond, 1987.

Nomenclature

Synonyms

abelia latent virus (Waterworth et al., 1975).

Acronym

EMV

ICTV decimal code

77.0.1.0.007

Host range and symptoms

Natural host range and symptoms

Symptoms persist.
• Solanum melongena - mosaic.
• Solanum seaforthianum - mottle, veinbanding.
• Lycopersicon esculentum - mosaic.

Transmission

Transmitted by a vector; an insect; Epitrix sp.; Coleoptera. Virus transmitted by mechanical inoculation; transmitted by seed.

Ecology and control

Studies reported by Gibbs et al. (1966); Debrot et al. (1977).

Geographical distribution

Spreads in Trinidad and Tobago.

Experimental host range

Few (<3) families susceptible.

Diagnostically susceptible host species and symptoms

• Chenopodium amaranticolor, C. quinoa - chlorotic or necrotic local lesions, then systemic mottle or mosaic.
• Cucumis sativus - symptomless, local infection only.
• Nicotiana clevelandii - necrotic local lesions, then systemic mottling and, sometimes, necrosis, stunting.
• Nicotiana glutinosa - chlorotic or necrotic local lesions, then systemic chlorotic or necrotic lesions or flecks and malformation.

Diagnostically insusceptible host species

Phaseolus vulgaris cvs The Prince, Bountiful, Stringless Green Pod and Top Crop.

Maintenance and propagation hosts

Nicotiana clevelandii, N. glutinosa.

Assay hosts (Local lesions or Whole plants)

Chenopodium amaranticolor (L), C. hybridum (L), Vigna unguiculata cv. Blackeye (L).

Susceptible host species

• Capsicum annuum
• Chenopodium album
• Chenopodium amaranticolor
• Chenopodium hybridum
• Chenopodium quinoa
• Cucumis sativus
• Datura stramonium
• Gomphrena globosa
• Lycopersicon esculentum
• Lycopersicon pimpinellifolium
• Nicandra physalodes
• Nicotiana clevelandii
• Nicotiana debneyi
• Nicotiana glutinosa
• Petunia × hybrida
• Physalis floridana
• Solanum melongena
• Solanum seaforthianum
• Solanum tuberosum
• Vigna unguiculata

Insusceptible host species

• Apium graveolens
• Cucumis sativus
• Cucurbita moschata
• Nicotiana rustica
• Nicotiana sylvestris
• Nicotiana tabacum
• Phaseolus vulgaris
• Zinnia elegans

Families containing susceptible hosts

• Amaranthaceae (1/1)
• Chenopodiaceae (4/4)
• Cucurbitaceae (1/2)
• Leguminosae-Papilionoideae (1/2)
• Solanaceae (13/16)

Families containing insusceptible hosts

• Compositae (1/1)
• Cucurbitaceae (2/2)
• Leguminosae-Papilionoideae (1/2)
• Solanaceae (3/16)
• Umbelliferae (1/1)

Sources of host-range data

Dale (1954); Debrot et al. (1977); Gibbs et al. (1966); Gibbs and Harrison (1969).

Physical and biochemical properties

Properties of particles in sap

TIP: 80-90 °C. LIV: 7 days. DEP: log₁₀ minus 7.

Purification method

Gibbs and Harrison (1973).

Particle morphology

Virions isometric; not enveloped; 30 nm in diameter; rounded in profile; with a conspicuous capsomere arrangement (capsid has 32 subunits).

Physical properties

Two sedimenting components in purified preparations; sedimentation coefficient of the fastest 111 S (B); of the other(s) 55 S (T). Isoelectric point pH 9.46.

Biochemical properties

Virions contain 36 % nucleic acid (B); 64 % protein (B); 0 % lipid.

Genome consists of RNA; single-stranded; linear. Total genome size 6.33 kb. Genome unipartite; largest (or only) genome part 6.33 kb. Genomic nucleic acid isolated by Bercks et al. (1971). Genome sequence reported by Osorio-Keese et al. (1989).

NCBI sequence data (Entrez search)

Sequence database accession code(s)

• J04374 Em(40)_vi:MTYRPVP Gb(84)_vi:EMVRPVP Eggplant mosaic virus genome. 5/90 6,331bp
• M10868 Em(40)_vi:TYMTY301 Gb(84)_vi:EMV3EMVA Eggplant mosaic virus RNA from the 3´ terminus. 7/89 59bp.
• M15285 Em(40)_vi:TYMTY3EM Gb(84)_vi:EMV3EMV Eggplant mosaic virus 3´ end which can form tRNA-like structure. 11/90 79bp
• M58314 Em(40)_vi:EMVRRLS Gb(84)_vi:EMVRRLS Eggplant mosaic virus (EMV) 3´ terminus tRNA-like structure. 8/91 80bp. 4 sequences.

Features of proteins

Virion protein(s) one; M_r 19815; coat protein (Dupin et al., 1984). Method of preparation: Bercks et al. (1971). Amino acid composition: Gibbs and Harrison (1973) and Dupin et al. (1984).

Replication

Genome replicates in association with chloroplasts (in vesicles of the membrane). Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm, in nuclei, in chloroplasts, in mitochondria, and in cell vacuoles. Inclusions present in infected cells; are unusual in shape; crystals in the vacuole; they contain virions.

Taxonomy and relationships

Tymovirus

Virus(es) with serologically related virions

Andean potato latent virus is closely related; belladonna mottle, dulcamara mottle, ononis yellow mosaic and scrophularia mottle viruses are more distantly related (Bercks et al., 1971).

Comments and References

References

• Dale, W.T. (1954). Ann. appl. Biol. 41: 240.
• Debrot, E.A., Lastra, R. and de Uzcátegui, R.C. (1977). Pl. Dis. Reptr 61: 628.
• Dupin, A., Peter, R., Collot, D., Das, B.C., Peter, C., Bouillon, P. and Duranton, H. (1984). C.R. hebd. Séanc. Acad. Sci., Paris, Serie III No. 8: 24.
• Ferguson, I.A.C. (1951). Pl. Dis. Reptr 35: 102.
• Gibbs, A.J. and Harrison, B.D. (1969). Ann. appl. Biol. 64: 225.
• Gibbs, A.J. and Harrison, B.D. (1973). CMI/AAB Descr. Pl. Viruses No. 124, 4 pp.
• Gibbs, A.J., Hecht-Poinar, E., Woods, R.D. and McKee, R.K. (1966). J. gen. Microbiol. 44: 177.
• Osorio-Keese, M., Keese, P. and Gibbs, A.J. (1989). Virology 172: 547.
• Waterworth, H.E., Kaper, J.M. and Koenig, R. (1975). Phytopathology 65: 891.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.