Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Dioscorea latent (?) potexvirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by S. Phillips, A.A. Brunt, 1985 and F. Nienhaus, 1987.

Nomenclature

Acronym

DLV

ICTV decimal code

56.0.1.T.008

Host range and symptoms

Natural host range and symptoms

• Dioscorea composita, D. floribunda - symptomless.

Transmission

Virus transmitted by mechanical inoculation; not transmitted by contact between plants; not transmitted by seed; not transmitted by pollen.

Geographical distribution

Spreads in Puerto Rico.

Experimental host range

Several (3-9) families susceptible.

Diagnostically susceptible host species and symptoms

• Nicotiana megalosiphon, N. benthamiana - symptomless local and systemic infection.
• Philodendron speciosum, Phaseolus vulgaris cv. The Prince, Vigna unguiculata cv. Blackeye, Datura metel, D. stramonium, Nicotiana clevelandii, N. sylvestris, N. tabacum cv. Dutch A - local symptomless infection only.

Diagnostically insusceptible host species

Solanum tuberosum, Commelina diffusa, Chenopodium quinoa, Trifolium incarnatum.

Maintenance and propagation hosts

Nicotiana benthamiana, N. megalosiphon.

Assay hosts (Local lesions or Whole plants)

Nicotiana benthamiana (W), N. megalosiphon (W).

Susceptible host species

• Datura metel
• Datura stramonium
• Dioscorea bulbifera
• Dioscorea composita
• Dioscorea floribunda
• Nicotiana benthamiana
• Nicotiana clevelandii
• Nicotiana megalosiphon
• Nicotiana plumbaginifolia
• Nicotiana sylvestris
• Nicotiana tabacum (cv. Dutch A)
• Phaseolus vulgaris
• Philodendron speciosum
• Tetragonia tetragonioides
• Vigna unguiculata

Insusceptible host species

• Brassica campestris ssp. pekinensis
• Capsicum annuum
• Chenopodium capitatum
• Chenopodium murale
• Chenopodium quinoa
• Colocasia esculenta
• Commelina diffusa
• Cucumis sativus
• Dioscorea alata
• Gomphrena globosa
• Nicotiana glutinosa
• Nicotiana sylvestris
• Nicotiana tabacum
• Oryza sativa
• Philodendron selloum
• Solanum tuberosum
• Trifolium incarnatum
• Vicia faba
• Zea mays
• Zinnia elegans

Families containing susceptible hosts

• Araceae (1/3)
• Dioscoreaceae (3/4)
• Leguminosae-Papilionoideae (2/4)
• Solanaceae (8/11)
• Tetragoniaceae (1/1)

Families containing insusceptible hosts

• Amaranthaceae (1/1)
• Araceae (2/3)
• Chenopodiaceae (3/3)
• Commelinaceae (1/1)
• Compositae (1/1)
• Cruciferae (1/1)
• Cucurbitaceae (1/1)
• Dioscoreaceae (1/4)
• Gramineae (2/2)
• Leguminosae-Papilionoideae (2 /4)
• Solanaceae (5/11)

Comments on host-range

Virus symptomless in Nicotiana plumbaginifolia and Dioscorea bulbifera, but does not infect D. alata.

Sources of host-range data

Phillips et al. (1986).

Physical and biochemical properties

Properties of particles in sap

TIP: 75-80 °C (in Nicotiana megalosiphon sap). LIV: 365 days (or more). DEP: log₁₀ minus 6 (in Nicotiana megalosiphon sap). Leaf sap contains many virions.

Purification method

Phillips et al. (1986).

Particle morphology

Virions filamentous; not enveloped; usually flexuous; with a clear modal length; of 445 nm; 11 nm wide. Axial canal obscure. Basic helix obscure.

Physical properties

Two sedimenting components in purified preparations (monomers and dimers); sedimentation coefficient of the fastest 131 S; of the other(s) 111 S. Density 1.33 g cm^-3 in CsCl (unfixed).

Biochemical properties

Virions contain 5 % nucleic acid; 95 % protein; 0 % lipid.

Genome consists of RNA; single-stranded. Total genome size 7.5 kb. Genome unipartite; largest (or only) genome part 7.5 kb. Genomic nucleic acid isolated by Phillips et al. (1986).

Features of the genome

Non-genomic nucleic acid not found in the virions.

Features of proteins

Virion protein(s) one; M_r 24900 (but reduced to 23100 by proteolysis). Method of preparation: Phillips et al. (1986).

Replication

Replication does not depend on a helper virus.

Cytopathology

Virions found in all parts of the host plant; in cytoplasm. Inclusions present in infected cells; are amorphous X-bodies and viroplasms; they contain virions.

Taxonomy and relationships

Possible Potexvirus

Virus(es) with serologically related virions

Commelina X and lily X potexviruses.

Virus(es) with serologically unrelated virions

Cactus X, cymbidium mosaic, hydrangea ringspot, narcissus mosaic, nerine X, papaya mosaic, pepino mosaic, potato X, potato aucuba mosaic, viola mottle and white clover mosaic viruses.

Comments and References

References

• Hearon, S.S., Corbett, M.K., Lawson, R.H., Gillespie, A.G. and Waterworth, H.E. (1978). Phytopathology 68: 1137.
• Phillips, S. and Brunt, A.A. (1988). CMI/AAB Descr. Pl. Viruses No. 335, 2 pp.
• Phillips, S., Piggott, J.D'A. and Brunt A.A. (1986). Ann. appl. Biol. 109: 137.
• Waterworth, H.E., Lawson, R.H. and Kahn, R.P. (1974). J. Agric. Univ. P. Rico 58: 351.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.