Plant Viruses Online   

Descriptions and Lists from the VIDE Database

Datura yellow vein nucleorhabdovirus

Index

• Nomenclature
• Host range, transmission and symptoms
• Physical and biochemical properties
• Taxonomy and relationships
• Comments and References

Data collated by J.E. Thomas, 1990.

Nomenclature

Acronym

DYVV

Strains

Thunbergia rhabdovirus.

ICTV decimal code

62.0.5.0.002

Host range and symptoms

Natural host range and symptoms

Symptoms persist. Symptoms vein yellowing and leaf malformation.
• Datura stramonium - vein yellowing, leaf and flower bud malformation.
• Thunbergia alata - vein clearing and yellowing, leaf malformation.
• Lycopersicon esculentum - vein clearing and chlorosis, mottling and leaf malformation.

Transmission

Not transmitted by Myzus persicae, Macrosiphum euphorbiae, Aphis gossypii, Acyrthosiphon (Aulacorthum) solani, Orosius argentatus, Austroasca alfalfae, Henosepilachna sp. Virus not transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between plants; not transmitted by seed.

Geographical distribution

Spreads in Australia.

Experimental host range

Few (<3) families susceptible. Experimentally infected plants mostly show vein yellowing.

Diagnostically susceptible host species and symptoms

• Datura stramonium - vein yellowing, leaf and flower bud malformation.
• Thunbergia alata - vein yellowing and leaf malformation.
• Lycopersicon esculentum - vein clearing and leaf malformation.
• Solanum melongena - vein etching.
• Nicotiana glutinosa - vein yellowing.
• Nicotiana tabacum - symptomless.

Diagnostically insusceptible host species

Capsicum annuum, Petunia × hybrida, Nicotiana rustica, N. physalodes, Solanum tuberosum cv. Sebago.

Maintenance and propagation hosts

Datura stramonium, Lycopersicon esculentum.

Assay hosts (Local lesions or Whole plants)

Datura stramonium (W).

Susceptible host species

• Datura stramonium
• Lycopersicon esculentum
• Nicotiana glutinosa
• Nicotiana tabacum
• Solanum melongena
• Thunbergia alata

Insusceptible host species

• Capsicum annuum
• Nicandra physalodes
• Nicotiana physalodes
• Nicotiana rustica
• Petunia × hybrida
• Solanum tuberosum

Families containing susceptible hosts

• Solanaceae (5/11)
• Thunbergiaceae (1/1)

Families containing insusceptible hosts

• Solanaceae (6/11)

Physical and biochemical properties

Properties of particles in sap

Leaf sap contains few virions.

Purification method

Thomas and Dietzgen (1991).

Particle morphology

Virions rhabdo- or bullet-shaped; enveloped; usually straight; with a clear modal length; of 166 nm; 77 nm wide. Basic helix obscure.

Features of proteins

Virion protein(s) four; M_r of the largest 78000; G protein. M_r of 2nd largest 47000; N protein. M_r of 3rd largest 41000; M₁ protein. M_r of 4th largest 36000; M₂ protein. Method of preparation: Thomas and Dietzgen (1991). Virion proteins glycosylated; are the G protein is glycosylated and binds to concanavalin A (lectin).

Cytopathology

Virions found in leaves; in the perinuclear space. Inclusions absent from infected cells.

Taxonomy and relationships

Nucleorhabdovirus: Rhabdoviridae

Virus(es) with serologically related virions

Sonchus yellow net virus.

Virus(es) with serologically unrelated virions

Potato yellow dwarf, potato chlorotic stunt, tomato vein yellowing, maize sterile stunt, cereal chlorotic mottle, digitaria striate, festuca leaf streak, lettuce necrotic yellows, northern cereal mosaic, maize mosaic and barley yellow striate mosaic viruses.

Best tests for diagnosis

Virion shape in negatively stained sap, characteristic symptoms in Datura stramonium, detection of viral protein from crude sap in Western immunoblots and gel diffusion serology.

Comments and References

References

• Dietzgen, R.G. and Thomas, J.E. (1990). Abstr. 8th Int. Congr. Virol., Berlin. p. 444.
• Thomas, J.E. and Behncken, G.M. (1983). Abstr. 4th Int. Congr. Pl. Path., Melbourne. p. 123.
• Thomas, J.E. and Dietzgen, R.G. (1991). Ann. appl. Biol. 118: 339.

Cite this publication as: Brunt, A.A., Crabtree, K., Dallwitz, M.J., Gibbs, A.J., Watson, L. and Zurcher, E.J. (eds.) (1996 onwards). `Plant Viruses Online: Descriptions and Lists from the VIDE Database. Version: 20^th August 1996.' URL http://biology.anu.edu.au/Groups/MES/vide/

Dallwitz (1980) and Dallwitz, Paine and Zurcher (1993) should also be cited.